Characterization of polymorphisms in the toxin A and B genes of Clostridium difficile

FEMS Microbiology Letters

Volumn 148, Issue 2, 1997, Pages 197-202

  Rupnik, Maja ^a,b   Braun, Veit ^a   Soehn, Florian ^a   Jane, Miha ^b   Hofstetter, Monika ^a   Laufenberg Feldmann, Rita ^a   Von Eichel Streiber, Christoph ^a  

^a JOHANNES GUTENBERG UNIVERSITY   (Germany)

^b UNIVERSITY OF LJUBLJANA   (Slovenia)

Author keywords

Clostridium difficile; genetic polymorphism; polymerase chain reaction; toxin A; toxin B

Indexed keywords

CLOSTRIDIUM DIFFICILE TOXIN A; CLOSTRIDIUM DIFFICILE TOXIN B;

ARTICLE; CLOSTRIDIUM DIFFICILE; GENE AMPLIFICATION; GENETIC POLYMORPHISM; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

BACTERIAL PROTEINS; BACTERIAL TOXINS; CLOSTRIDIUM DIFFICILE; ENTEROTOXINS; GENES, BACTERIAL; POLYMERASE CHAIN REACTION; POLYMORPHISM, GENETIC; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH;

EID: 0030932086     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(97)00033-5     Document Type: Article

References (18)

1. Knoop, F.C., Owens, M. and Crocker, I.C. (1993) Clostridium difficile: clinical disease and diagnosis. Clin. Microbiol. Rev. 6, 251-265.
2. Von Eichel-Streiber, C. (1993) Molecular biology of the Clostridium difficile toxins. In: Genetics and Molecular Biology of Anaerobic Bacteria (Sebald M., Ed.), Springer, Berlin.
3. Von Eichel-Streiber, C., Boquet, P., Sauerborn, M. and Thelestam, M. (1996) Large clostridial cytotoxins: a family of glycosyltransferases modifying small GTP-binding proteins. TIM 4, 375-382.
4. Braun, V., Hundsberger, T., Leukel, P., Sauerborn, M. and Von Eichel-Streiber, C. (1996) Definition of the single integration site of the pathogenicity locus in Clostridium difficile. Gene 181, 29-38.
5. Borriello, S.P., Wren, B.W., Hyde, S., Seddon, S.V., Sibbons, P., Krishna, M.M., Tabaqchali, S., Manek, S., Price, A.B. (1992) Molecular, immunological, and biological characterization of a Toxin A-negative, Toxin B-positive strain of Clostridium difficile, Infect. Immunol. 60, 4192-4199.
6. Lyerly, D.M., Barroso, L.A., Wilkins, T.D., Depitre, C. and Corthier, G. (1992) Characterization of a Toxin A-negative, Toxin B-positive strain of Clostridium difficile. Infect. Immun. 60, 4633-4639.
7. Depitre, C., Delmee, M., Avesani, V., L'Haridon, R., Roels, A., Popoff, M. and Corthier, G. (1993) Serogroup F strains of Clostridium difficile produce toxin B but not toxin A. J. Med. Microbiol. 38, 434-441.
8. Von Eichel-Streiber, C., Meyer zu Heringdorf, D., Habermann, E. and Sartingen, S. (1995) Closing in on the toxic domain through analysis of a variant Clostridium difficile cytotoxin B. Mol. Microbiol. 17, 315-321.
9. Torres, J.F. (1991) Purification and characterisation of toxin B from a strain of Clostridium difficile that does not produce toxin A. J. Med. Microbiol. 35, 40-44.
10. Von Eichel-Streiber, C. and Sauerborn, M. (1995) Molecular variations of toxins A and B: implications for the diagnosis of Clostridium difficile-induced disease. In: Medical and Dental Aspects of Anaerobes (Duerden, B.I., Wade, W.G., Brazier, J.S., Eley, A., Wren, B. and Hudson, M.J., eds.), Science Reviews, Northwood.
11. Delmée, M. and Avesani, V. (1990) Virulence of ten serogroups of Clostridium difficile in hamsters. J. Med. Microbiol. 33, 85-90.
12. Wren, B.W., Clayton, C.L. and Tabaqchali, S. (1990) Nucleotide sequence of Clostridium difficile toxin A gene fragment and detection of toxigenic strains by polymerase chain reaction. FEMS Microbiol. Lett. 70, 1-6.
13. Fluit, A.C., Wolfhagen, M.J., Verdonk, G.P., Jansze, M., Torensma, R. and Verhoef, J. (1991) Nontoxigenic strains of Clostridium difficile lack the genes for both toxin A and toxin B. J. Clin. Microbiol. 29, 2666-2667.
14. Kato, N., Ou, C.-Y., Kato, H., Bartley, S.L., Brown, V.K., Dowell, Jr. V.R. and Ueno, K. (1991) Identification of toxigenic Clostridium difficile by the polymerase chain reaction. J. Clin. Microbiol. 29, 33-37.
15. Boondeekhun, H.S., Gurtler, V., Odd, M.L., Wilson, V.A. and Mayall, B.C. (1993) Detection of Clostridium difficile enterotoxin gene in clinical specimens by polymerase chain reaction. J. Med. Microbiol. 38, 384-387.
16. Gumerlock, P.H., Tang, Y.J., Weis, J.B., and Silva, Jr. J. (1993) Specific detection of toxinogenic strains of in stool specimens. J. Clin. Microbiol. 31, 507-511.
17. Von Eichel-Streiber, C. and Sauerborn, M. (1990) Clostridium difficile toxin A carries a C-terminal repetitive structure homologous to the carbohydrate binding region of streptococcal glycosyltransferases. Gene 96, 107-113.
18. Lyerly, D.M., Saum, K.E., MacDonald, D.K., Wilkins, T.D. (1985) Effects of Clostridium difficile toxins given intragastrically to animals. Infect. Immun. 47, 349-352.