Engineering Rhodosporidium toruloides with a membrane transporter facilitates production and separation of carotenoids and lipids in a bi-phasic culture

Applied Microbiology and Biotechnology

Volumn 100, Issue 2, 2016, Pages 869-877

  Lee, Jaslyn J L ^a   Chen, Liwei ^b   Cao, Bin ^c   Chen, Wei Ning ^a,b  

^a NANYANG TECHNOLOGICAL UNIVERSITY   (Singapore)

^b Nanyang Technological University   (Singapore)

^c NANYANG TECHNOLOGICAL UNIVERSITY   (Singapore)

Author keywords

ABC transporter; Carotenoids; Extraction; Lipids; Rhodosporidium toruloides; Separation

Indexed keywords

CHROMATOGRAPHY; CYTOLOGY; EXTRACTION; GAS CHROMATOGRAPHY; LIPIDS; MASS SPECTROMETRY; OILS AND FATS; PIGMENTS; SEPARATION; UNSATURATED FATTY ACIDS;

ABC TRANSPORTER; BIOTECHNOLOGICAL POTENTIALS; CAROTENOIDS; GAS CHROMATOGRAPHY-MASS SPECTROMETRIES (GC-MS); INTRACELLULAR GLYCEROL; OLEAGINOUS YEASTS; PHARMACEUTICAL INDUSTRY; RHODOSPORIDIUM TORULOIDES;

FATTY ACIDS;

ABC TRANSPORTER; CAROTENOID; FATTY ACID; GLYCEROL; PLEIOTROPIC DRUG RESISTANCE 10; UNCLASSIFIED DRUG; VEGETABLE OIL; BIOFUEL; CARRIER PROTEIN; CULTURE MEDIUM; OIL; TORULENE; UNSATURATED FATTY ACID;

CAROTENOID; FATTY ACID; HYDROPHOBICITY; LIPID; MEMBRANE; SEPARATION; YEAST;

ARTICLE; CONTROLLED STUDY; LIPID COMPOSITION; MASS FRAGMENTOGRAPHY; NONHUMAN; RHODOSPORIDIUM TORULOIDES; BASIDIOMYCETES; BIOSYNTHESIS; CHEMISTRY; CULTURE MEDIUM; FERMENTATION; GENETIC ENGINEERING; GENETICS; ISOLATION AND PURIFICATION; METABOLISM;

RHODOSPORIDIUM TORULOIDES; VITACEAE;

BASIDIOMYCOTA; BIOFUELS; CAROTENOIDS; CULTURE MEDIA; FATTY ACIDS; FATTY ACIDS, UNSATURATED; FERMENTATION; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; GENETIC ENGINEERING; GLYCEROL; MEMBRANE TRANSPORT PROTEINS; OILS;

EID: 84953635977     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-015-7102-3     Document Type: Article

References (30)

1. Baumgartner K, Fujiyoshi P, Foster GD, Bailey AM (2010) Agrobacterium tumefaciens-mediated transformation for investigation of somatic recombination in the fungal pathogen Armillaria mellea. Appl Environ Microbiol 76(24):7990–7996. doi:10.1128/aem.01049-10
2. Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem and Phys 37(8):911–917
3. Buzzini P, Innocenti M, Turchetti B, Libkind D, van Broock M, Mulinacci N (2007) Carotenoid profiles of yeasts belonging to the genera Rhodotorula, Rhodosporidium, Sporobolomyces, and Sporidiobolus. Can J Microbiol 53(8):1024–1031. doi:10.1139/W07-068
4. Chatzifragkou A, Dietz D, Komaitis M, Zeng AP, Papanikolaou S (2010) Effect of biodiesel-derived waste glycerol impurities on biomass and 1,3-propanediol production of Clostridium butyricum VPI 1718. Biotech and Bioeng 107(1):76–84
5. Chuang M-H, Brunner G (2006) Concentration of minor components in crude palm oil. J Supercrit Fluids 37(2):151–156. doi:10.1016/j.supflu.2005.09.004
6. De Marchi F, Seraglia R, Molin L, Traldi P, De Rosso M, Panighel A, Dalla Vedova A, Gardiman M, Giust M, Flamini R (2012) Seed oil triglyceride profiling of thirty-two hybrid grape varieties. J Mass Spect 47(9):1113–1119. doi:10.1002/jms.3010
7. Fickers P, Benetti PH, Wache Y, Marty A, Mauersberger S, Smit MS, Nicaud JM (2005) Hydrophobic substrate utilisation by the yeast Yarrowia lipolytica, and its potential applications. FEMS Yeast Res 5(6–7):527–543. doi:10.1016/j.femsyr.2004.09.004
8. Frengova G, Beshkova D (2009) Carotenoids from Rhodotorula and Phaffia: yeasts of biotechnological importance. J Ind Microbiol & Biotech 36:163–180
9. Gruszecki WI, Strzałka K (2005) Carotenoids as modulators of lipid membrane physical properties. Biochim et Biophys Acta - Molecular Basis of Disease 1740(2):108–115. doi:10.1016/j.bbadis.2004.11.015
10. Guo X, Ota Y (2000) Incorporation of eicosapentaenoic and docosahexaenoic acids by a yeast (FO726A). J Appl Microbiol 89(1):107–115
11. Higgins CF (1992) ABC transporters: from microorganisms to man. Ann Rev of Cell Biol 8:67–113. doi:10.1146/annurev.cb.08.110192.000435
12. Horák T, Čulík J, Čejka P, Jurková M, Kellner V, Dvořák J, Hašková D (2009) Analysis of free fatty acids in beer: comparison of solid-phase extraction, solid-phase microextraction, and stir bar sorptive extraction. J Agri and Food Chem 57(23):11081–11085. doi:10.1021/jf9028305
13. Katre G, Joshi C, Khot M, Zinjarde S, Ravikumar A (2012) Evaluation of single cell oil (SCO) from a tropical marine yeast Yarrowia lipolytica NCIM 3589 as a potential feedstock for biodiesel. AMB Exp 2(1):36. doi:10.1186/2191-0855-2-36
14. Kohlwein SD, Paltauf F (1984) Uptake of fatty acids by the yeasts, Saccharomyces uvarum and Saccharomycopsis lipolytica. Biochim et Biophys Acta 792(3):310–317
15. Lee JJ, Chen L, Shi J, Trzcinski A, Chen WN (2014) Metabolomic profiling of Rhodosporidium toruloides grown on glycerol for carotenoid production during different growth phases. J Agric Food Chem 62(41):10203–10209. doi:10.1021/jf502987q
16. Li Y, Ghasemi Naghdi F, Garg S, Adarme-Vega TC, Thurecht KJ, Ghafor WA, Tannock S, Schenk PM (2014) A comparative study: the impact of different lipid extraction methods on current microalgal lipid research. Microb Cell Factories 13:14. doi:10.1186/1475-2859-13-14
17. Liu Y, Koh C, Sun L, Hlaing M, Du M, Peng N, Ji L (2013) Characterization of glyceraldehyde-3-phosphate dehydrogenase gene Rt GPD1 and development of genetic transformation method by dominant selection in oleaginous yeast Rhodosporidium toruloides. App Microbiol & Biotech 97(2):719–729. doi:10.1007/s00253-012-4223-9
18. Mata-Gomez L, Montanez J, Mendez-Zavala A, Aguilar C (2014) Biotechnological production of carotenoids by yeasts: an overview. Micro Cell Fact 13(1):12
19. Mosier N, Wyman C, Dale B, Elander R, Lee YY, Holtzapple M, Ladisch M (2005) Features of promising technologies for pretreatment of lignocellulosic biomass. Biores Tech 96(6):673–686. doi:10.1016/j.biortech.2004.06.025
20. Pande G, Akoh CC (2010) Organic acids, antioxidant capacity, phenolic content and lipid characterisation of Georgia-grown underutilized fruit crops. Food Chem 120(4):1067–1075. doi:10.1016/j.foodchem.2009.11.054
21. Park PK, Kim EY, Chu KH (2007) Chemical disruption of yeast cells for the isolation of carotenoid pigments. Sep and Purif Tech 53(2):148–152. doi:10.1016/j.seppur.2006.06.026
22. Peralta-Yahya PP, Zhang F, del Cardayre SB, Keasling JD (2012) Microbial engineering for the production of advanced biofuels. Nat 488(7411):320–328
23. Rodriguez-Amaya DB, Kimura M (2004) HarvestPlus handbook for carotenoid analysis. HarvestPlus, Washington
24. Sheehan J, Dunahay T, Benemann J, Roessler P (1998) Look back at the U.S. Department of Energy’s Aquatic Species Program: biodiesel from algae; close-out report other information: PBD: 1 Jul 1998. p Medium: ED; Size: 325 pages
25. Vakhlu J, Kour A (2006) Yeast lipases: enzyme purification, biochemical properties and gene cloning. Elect J Biotech 9(1). doi:10.2225/vol9-issue1-fulltext-9
26. Verwaal R, Jiang Y, Wang J, Daran JM, Sandmann G, van den Berg JA, van Ooyen AJ (2010) Heterologous carotenoid production in Saccharomyces cerevisiae induces the pleiotropic drμg resistance stress response. Yeast 27(12):983–998. doi:10.1002/yea.1807
27. Wang M, Bai J, Chen WN, Ching CB (2010) Metabolomic profiling of cellular responses to carvedilol enantiomers in vascular smooth muscle cells. PLoS One 5(11)
28. Weber RW, Anke H, Davoli P (2007) Simple method for the extraction and reversed-phase high-performance liquid chromatographic analysis of carotenoid pigments from red yeasts (Basidiomycota, fungi). J Chrom A 1145:118–122. doi:10.1016/j.chroma.2007.01.052
29. Xu J, Zhao X, Wang W, Du W, Liu D (2012) Microbial conversion of biodiesel byproduct glycerol to triacylglycerols by oleaginous yeast Rhodosporidium toruloides and the individual effect of some impurities on lipid production. Biochem Engin J 65:30–36
30. Zhu Z, Zhang S, Liu H, Shen H, Lin X, Yang F, Zhou YJ, Jin G, Ye M, Zou H, Zhao ZK (2012) A multi-omic map of the lipid-producing yeast rhodosporidium toruloides. Nat Comm 3