A genome-wide CRISPR (clustered regularly interspaced short palindromic repeats) screen identifies NEK7 as an essential component of NLRP3 inflammasome activation

Journal of Biological Chemistry

Volumn 291, Issue 1, 2016, Pages 103-109

  Schmid Burgk, Jonathan L ^a   Chauhan, Dhruv ^a   Schmidt, Tobias ^a   Ebert, Thomas S ^a   Reinhardt, Julia ^a   Endl, Elmar ^a   Hornung, Veit ^a,b  

^a UNIVERSITY HOSPITAL BONN   (Germany)

^b UNIVERSITY OF MUNICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CELL DEATH; CHEMICAL ACTIVATION; GENES; MACROPHAGES;

ALZHEIMER DISEASE; GENE PRODUCTS; HIGH MOLECULAR WEIGHT PROTEIN; INFLAMMATORY CONDITIONS; MOLECULAR MECHANISM; MURINE MACROPHAGES; POTASSIUM EFFLUX; TYPE-2 DIABETES;

MOLECULAR INTERACTIONS;

CRYOPYRIN; CYTOKINE; GUIDE RNA; INFLAMMASOME; INTERLEUKIN 1BETA CONVERTING ENZYME; NIGERICIN; POTASSIUM; CARRIER PROTEIN; NEK7 PROTEIN, MOUSE; NIMA RELATED KINASE; NLRP3 PROTEIN, MOUSE; PROTEIN SERINE THREONINE KINASE;

ALZHEIMER DISEASE; ARTICLE; ATHEROSCLEROSIS; CELL DEATH; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONTROLLED STUDY; CYTOSOL; FLUORESCENCE ACTIVATED CELL SORTING; GENE PRODUCT; GENETIC ASSOCIATION; GOUT; HUMAN; INNATE IMMUNITY; LETHALITY; MACROPHAGE; MOLECULAR MECHANICS; MOLECULAR WEIGHT; MOUSE; NON INSULIN DEPENDENT DIABETES MELLITUS; NONHUMAN; PATTERN RECOGNITION; POTASSIUM TRANSPORT; PRIORITY JOURNAL; PYROPTOSIS; ANIMAL; GENETICS; GENOME; HEK293 CELL LINE; METABOLISM; SIGNAL TRANSDUCTION;

ANIMALS; CARRIER PROTEINS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; GENOME; HEK293 CELLS; HUMANS; INFLAMMASOMES; MICE; NIMA-RELATED KINASES; NLR FAMILY, PYRIN DOMAIN-CONTAINING 3 PROTEIN; PROTEIN-SERINE-THREONINE KINASES; SIGNAL TRANSDUCTION;

EID: 84952931119     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.C115.700492     Document Type: Article

References (27)

1. Medzhitov, R. (2007) Recognition of microorganisms and activation of the immune response. Nature 449, 819-826
2. Chen, G. Y., and Nuñez, G. (2010) Sterile inflammation: sensing and reacting to damage. Nat. Rev. Immunol. 10, 826-837
3. Gross, O., Thomas, C. J., Guarda, G., and Tschopp, J. (2011) The inflammasome: an integrated view. Immunol. Rev. 243, 136-151
4. Kayagaki, N., Stowe, I. B., Lee, B. L., O'Rourke, K., Anderson, K., Warming, S., Cuellar, T., Haley, B., Roose-Girma, M., Phung, Q. T., Liu, P. S., Lill, J. R., Li, H., Wu, J., Kummerfeld, S., Zhang, J., Lee, W. P., Snipas, S. J., Salvesen, G. S., Morris, L. X., Fitzgerald, L., Zhang, Y., Bertram, E. M., Goodnow, C. C., and Dixit, V. M. (2015) Caspase-11 cleaves gasdermin D for noncanonical inflammasome signaling. Nature 526, 666-671
5. Shi, J., Zhao, Y., Wang, K., Shi, X., Wang, Y., Huang, H., Zhuang, Y., Cai, T., Wang, F., and Shao, F. (2015) Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death. Nature 526, 660-665
6. Latz, E., Xiao, T. S., and Stutz, A. (2013) Activation and regulation of the inflammasomes. Nat. Rev. Immunol. 13, 397-411
7. Masters, S. L., Latz, E., and O'Neill, L. A. (2011) The inflammasome in atherosclerosis and type 2 diabetes. Sci. Transl. Med. 3, 81ps17
8. Bauernfeind, F. G., Horvath, G., Stutz, A., Alnemri, E. S., MacDonald, K., Speert, D., Fernandes-Alnemri, T., Wu, J., Monks, B. G., Fitzgerald, K. A., Hornung, V., and Latz, E. (2009) Cutting edge: NF-κB activating pattern recognition and cytokine receptors license NLRP3 inflammasome activation by regulating NLRP3 expression. J. Immunol. 183, 787-791
9. Franklin, B. S., Bossaller, L., De Nardo, D., Ratter, J. M., Stutz, A., Engels, G., Brenker, C., Nordhoff, M., Mirandola, S. R., Al-Amoudi, A., Mangan, M. S., Zimmer, S., Monks, B. G., Fricke, M., Schmidt, R. E., Espevik, T., Jones, B., Jarnicki, A. G., Hansbro, P. M., Busto, P., Marshak-Rothstein, A., Hornemann, S., Aguzzi, A., Kastenmüller, W., and Latz, E. (2014) The adaptor ASC has extracellular and 'prionoid' activities that propagate inflammation. Nat. Immunol. 15, 727-737
10. Juliana, C., Fernandes-Alnemri, T., Kang, S., Farias, A., Qin, F., and Alnemri, E. S. (2012) Non-transcriptional priming and deubiquitination regulate NLRP3 inflammasome activation. J. Biol. Chem. 287, 36617-36622
11. Schroder, K., Sagulenko, V., Zamoshnikova, A., Richards, A. A., Cridland, J. A., Irvine, K. M., Stacey, K. J., and Sweet, M. J. (2012) Acute lipopolysaccharide priming boosts inflammasome activation independently of inflammasome sensor induction. Immunobiology 217, 1325-1329
12. Pétrilli, V., Papin, S., Dostert, C., Mayor, A., Martinon, F., and Tschopp, J. (2007) Activation of the NALP3 inflammasome is triggered by low intracellular potassium concentration. Cell Death Differ. 14, 1583-1589
13. Muñoz-Planillo, R., Kuffa, P., Martínez-Colón, G., Smith, B. L., Rajendiran, T. M., and Núñez, G. (2013) K+ efflux is the common trigger of NLRP3 inflammasome activation by bacterial toxins and particulate matter. Immunity 38, 1142-1153
14. Schmidt, T., Schmid-Burgk, J. L., and Hornung, V. (2015) Synthesis of an arrayed sgRNA library targeting the human genome. Sci. Rep. 5, 14987
15. Schmid-Burgk, J. L., Schmidt, T., Gaidt, M. M., Pelka, K., Latz, E., Ebert, T. S., and Hornung, V. (2014) OutKnocker: a web tool for rapid and simple genotyping of designer nuclease edited cell lines. Genome Res. 24, 1719-1723
16. Schmidt, T., Schmid-Burgk, J. L., Ebert, T. S., Gaidt, M. M., and Hornung, V. (2016) Designer nuclease-mediated generation of knockout THP1 cells. Methods Mol. Biol. 1338, 261-272
17. Jakobs, C., Bartok, E., Kubarenko, A., Bauernfeind, F., and Hornung, V. (2013) Immunoblotting for active caspase-1. Methods Mol. Biol. 1040, 103-115
18. Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott, D. A., Mikkelsen, T. S., Heckl, D., Ebert, B. L., Root, D. E., Doench, J. G., and Zhang, F. (2014) Genome-scale CRISPR-Cas9 knockout screening in human cells. Science 343, 84-87
19. Wang, T., Wei, J. J., Sabatini, D. M., and Lander, E. S. (2014) Genetic screens in human cells using the CRISPR-Cas9 system. Science 343, 80-84
20. Wu, C., Orozco, C., Boyer, J., Leglise, M., Goodale, J., Batalov, S., Hodge, C. L., Haase, J., Janes, J., Huss, J. W., 3rd, and Su, A. I. (2009) BioGPS: an extensible and customizable portal for querying and organizing gene annotation resources. Genome Biol. 10, R130
21. Misawa, T., Takahama, M., Kozaki, T., Lee, H., Zou, J., Saitoh, T., and Akira, S. (2013) Microtubule-driven spatial arrangement of mitochondria promotes activation of the NLRP3 inflammasome. Nat. Immunol. 14, 454-460
22. Cohen, S., Aizer, A., Shav-Tal, Y., Yanai, A., and Motro, B. (2013) Nek7 kinase accelerates microtubule dynamic instability. Biochim. Biophys. Acta 1833, 1104-1113
23. O'Regan, L., and Fry, A. M. (2009) The Nek6 and Nek7 protein kinases are required for robust mitotic spindle formation and cytokinesis. Mol. Cell. Biol. 29, 3975-3990
24. Chang, J., Baloh, R. H., and Milbrandt, J. (2009) The NIMA-family kinase Nek3 regulates microtubule acetylation in neurons. J. Cell Sci. 122, 2274-2282
25. Salem, H., Rachmin, I., Yissachar, N., Cohen, S., Amiel, A., Haffner, R., Lavi, L., and Motro, B. (2010) Nek7 kinase targeting leads to early mortality, cytokinesis disturbance and polyploidy. Oncogene 29, 4046-4057
26. Quarmby, L. M., and Mahjoub, M. R. (2005) Caught Nek-ing: cilia and centrioles. J. Cell Sci. 118, 5161-5169
27. Fry, A. M., O'Regan, L., Sabir, S. R., and Bayliss, R. (2012) Cell cycle regulation by the NEK family of protein kinases. J. Cell Sci. 125, 4423-4433