Designer nuclease-mediated generation of knockout THP1 cells

Methods in Molecular Biology

Volumn 1338, Issue , 2016, Pages 261-272

  Schmidt, Tobias ^a   Schmid Burgk, Jonathan L ^a   Ebert, Thomas S ^a   Gaidt, Moritz M ^a   Hornung, Veit ^a  

^a NONE

Author keywords

Barcoded deep sequencing; CRISPR Cas9; Genome editing; Genotyping; Knockout cell lines

Indexed keywords

NUCLEASE; ENDONUCLEASE; CRISPR ASSOCIATED ENDONUCLEASE CAS9; GENOMIC DNA;

ALLELE; CELL CLONE; CELL CLONING; CELL SUSPENSION; ELECTROPORATION; FRAMESHIFT MUTATION; GENE; GENOTYPE; HUMAN; HUMAN CELL; LEUKEMIA CELL; THP1 MONOCYTIC LEUKEMIA CELL; CELL LINE; CRISPR CAS SYSTEM; DOUBLE STRANDED DNA BREAK; GENE INACTIVATION; GENETICS; GENOME; HIGH THROUGHPUT SEQUENCING; INDEL MUTATION; PROCEDURES; AMPLICON; CRISPR-CAS9 SYSTEM; DILUTION; FLUORESCENCE ACTIVATED CELL SORTING; GENE EDITING; GENE KNOCKOUT; GENOTYPING; PLASMID; THP-1 CELL LINE;

CELL LINE; CRISPR-CAS SYSTEMS; DNA BREAKS, DOUBLE-STRANDED; ENDONUCLEASES; FRAMESHIFT MUTATION; GENE KNOCKOUT TECHNIQUES; GENOME; GENOTYPE; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; INDEL MUTATION;

EID: 84943560954     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2932-0_19     Document Type: Chapter

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