Correlative fluorescence and electron microscopy

Current Protocols in Cytometry

Volumn 2014, Issue , 2014, Pages 12.36.1-12.36.10

  Schirra, Randall T ^a   Zhang, Peijun ^a  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

Author keywords

Cell biology; Correlative; CryoEM; Electron microscopy; Fluorescence; Imaging; Immunolabel; Integrated microscope; Light microscopy; Tomography

Indexed keywords

FLUORESCENT DYE; FLUORONANOGOLD; GREEN FLUORESCENT PROTEIN; NANOPARTICLE; QUANTUM DOT; UNCLASSIFIED DRUG;

ARTICLE; CORRELATIVE FLUORESCENCE AND ELECTRON MICROSCOPY; ELECTRON MICROSCOPE; ELECTRON MICROSCOPY; ELECTRON TOMOGRAPHY; FLUORESCENCE MICROSCOPY; FREEZING; INTEGRATED FLUORESCENCE ELECTRON MICROSCOPE; INTERMETHOD COMPARISON; NONHUMAN; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; SERIAL BLOCK FACE SCANNING ELECTRON MICROSCOPY; TRANSMISSION ELECTRON MICROSCOPY; ANIMAL; COMPARATIVE STUDY; DEVICES; HUMAN; PROCEDURES;

ANIMALS; HUMANS; MICROSCOPY, ELECTRON; MICROSCOPY, FLUORESCENCE;

EID: 84949293168     PISSN: 19349297     EISSN: 19349300     Source Type: Journal    
DOI: 10.1002/0471142956.cy1236s70     Document Type: Article

References (74)

1. Abu-Lail, N.I., Ohashi, T., Clark, R.L., Erickson, H.P., and Zauscher, S. 2006. Understanding the elasticity of fibronectin fibrils: Unfolding strengths of FN-III and GFP domains measured by single molecule force spectroscopy. Matrix Biol. 25:175-184.
2. Agronskaia, A.V., Valentijn, J.A., van Driel, L.F., Schneijdenberg, C.T.W.M., Humbel, B.M., van Bergen, E.N., Henegouwen, P.M., Verkleij, A.J., Koster, A.J., and Gerritsen, H.C. 2008. Integrated fluorescence and transmission electron microscopy. J. Struct. Biol. 164:183-189.
3. Alivisatos, A.P., Gu, W., and Larabell, C. 2005. Quantum dots as cellular probes. Annu. Rev. Biomed. Eng. 7:55-76.
4. Armbruster, B.L., Carlemalm, E., Chiovetti, R., Garavito, R.M., Hobot, J.A., Kellenberger, E., and Villiger, W. 1982. Specimen preparation for electron microscopy using low temperature embedding resins. J. Microsc. 126:77-85.
5. Armbruster, B.L., Garavito, R.M., and Kellenberger, E. 1983. Dehydration and embedding temperatures affect the antigenic specificity of tubulin and immunolabeling by the protein Acolloidal gold technique. J. Histochem. Cytochem. 31:1380-1384.
6. Boassa, D., Berlanga, M.L., Yang, M.A., Terada, M., Hu, J., Bushong, E.A., Hwang, M., Masliah, E., George, J.M., and Ellisman, M.H. 2013. Mapping the subcellular distribution of a-synuclein in neurons using genetically encoded probes for correlated light and electron microscopy: Implications for Parkinson's disease pathogenesis. J. Neurosci. 33:2605-2615.
7. Bushong, E.A., Martone, M.E., Jones, Y.Z., and Ellisman, M.H. 2002. Protoplasmic astrocytes in CA1 stratum radiatum occupy separate anatomical domains. J. Neurosci. 22:183-192.
8. Carlemalm, E., Villiger, W., Hobot, J.A., Acetarin, J.-D., and Kellenberger, E. 1985. Low temperature embedding with Lowicryl resins: Two new formulations and some applications. J. Microsc. 140:55-63.
9. Clegg, R.M. 2009. Förster resonance energy transfer-FRET: What is it, why do it, and how it's done. FRET FLIM Techn. 1:1-57.
10. Cotte, Y., Toy, F., Jourdain, P., Pavillon, N., Boss, D., Magistretti, P., Marquet, P., and Depeursinge, C. 2013. Marker-free phase nanoscopy. Nat. Photonics 7:113-117.
11. Egerton, R.F. 2005. The scanning electron microscope. In Physical Principles of Electron Microscopy. pp. 125-128. Springer, New York.
12. Ellisman, M.H., Deerinck, T.J., Shu, X., and Sosinsky, G.E. 2012. Picking faces out of a crowd: Genetic labels for identification of proteins in correlated light and electron microscopy imaging. Methods Cell Biol. 111:139-153.
13. Faas, F.G.A., Bárcena, M., Agronskaia, A.V., Gerritsen, H.C., Moscicka, K.B., Diebolder, C.A., van Driel, L.F., Limpens, R.W.A.L., Bos, E., and Ravelli, R.B.G. 2013. Localization of fluorescently labeled structures in frozen-hydrated samples using integrated light electron microscopy. J. Struct. Biol. 181:283-290.
14. Fabig, G., Kretschmar, S., Weiche, S., Eberle, D., Ader, M., and Kurth, T. 2012. 5 Labeling of ultrathin resin sections for correlative light and electron microscopy. Methods Cell Biol. 111:75-91.
15. Fitzpatrick, A.W.P., Lorenz, U.J., Vanacore, G.M., and Zewail, A.H. 2013. 4D cryo-electron microscopy of proteins. J. Am. Chem. Soc. 135:19123-19126.
16. Gaietta, G., Deerinck, T.J., Adams, S.R., Bouwer, J., Tour, O., Laird, D.W., Sosinsky, G.E., Tsien, R.Y., and Ellisman, M.H. 2002. Multicolor and electron microscopic imaging of connexin trafficking. Science 296:503-507.
17. Gaietta, G.M., Deerinck, T.J., and Ellisman, M.H. 2011. Correlated live cell light and electron microscopy using tetracysteine tags and biarsenicals. Cold Spring Harb. Protoc. 2001:pdbprot5548.
18. Giepmans, B.N.G. 2006. The fluorescent toolbox for assessing protein location and function. Science 312:217-224.
19. Giepmans, B.G. 2008. Bridging fluorescence microscopy and electron microscopy. Histochem. Cell Biol. 130:211-217.
20. Grabenbauer, M., Geerts, W.J., Fernadez-Rodriguez, J., Hoenger, A., Koster, A.J., and Nilsson, T. 2005. Correlative microscopy and electron tomography of GFP through photooxidation. Nat. Methods 2:857-862.
21. Gustafsson, M.G. 2000. Surpassing the lateral resolution limit by a factor of two using structured illuminationmicroscopy. J. Microsc. 198:82-87.
22. Hell, S.W. and Wichmann, J. 1994. Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy. Optics Lett. 19:780-782.
23. Hess, S.T., Girirajan, T.P., and Mason, M.D. 2006. Ultra-high resolution imaging by fluorescence photoactivation localization microscopy. Biophys. J. 91:4258-4272.
24. Hoenger, A. 2014. High-resolution cryo-electron microscopy on macromolecular complexes and cell organelles. Protoplasma 251:217-227.
25. Hughes, L., Hawes, C., Monteith, S., and Vaughan, S. 2014. Serial block face scanning electron microscopy-the future of cell ultrastructure imaging. Protoplasma 251:395-401.
26. Idrissi, F.-Z. and Geli, M.I. 2013. Zooming in on the molecular mechanisms of endocytic budding by time-resolved electron microscopy. Cell. Mol. Life Sci. 1:8211-8217.
27. Juette, M.F., Gould, T.J., Lessard, M.D., Mlodzianoski, M.J., Nagpure, B.S., Bennett, B.T., Hess, S.T., and Bewersdorf, J. 2008. Threedimensional sub-100 nm resolution fluorescence microscopy of thick samples. Nat. Methods 5:527-529.
28. Jun, S., Ke, D., Debiec, K., Zhao, G., Meng, X., Ambrose, Z., Gibson, G.A., Watkins, S.C., and Zhang, P. 2011. Direct visualization of HIV-1 with correlative live-cell microscopy and cryoelectron tomography. Structure 19:1573-1581.
29. Jun, S., Zhao, G., Ning, J., Gibson, G.A., Watkins, S.C., and Zhang, P. 2013. Correlative microscopy for 3D structural analysis of dynamic interactions. J. Vis. Exp. doi:10.3791/50386.
30. Karreman, M.A., Agronskaia, A.V., van Donselaar, E.G., Vocking, K., Fereidouni, F., Humbel, B.M., Verrips, C.T., Verkleij, A.J., and Gerritsen, H.C. 2012. Optimizing immuno-labeling for correlative fluorescence and electron microscopy on a single specimen. J. Struct. Biol. 180:382-386.
31. Koster, A.J. and Bárcena, M. 2006. Cryotomography: Low-dose automated tomography of frozen-hydrated specimens. In Electron Tomography: Methods for Three-Dimensional Visualization of Structures in the Cell, 2nd ed. (J. Frank, ed.) pp. 126-129. Springer, New York.
32. Leung, B.O. andChou, K.C. 2011. Reviewof superresolution fluorescence microscopy for biology. Appl. Spectrosc. 65:967-980.
33. Lorenz, U.J. and Zewail, A.H. 2013. Biomechanics of DNA structures visualized by 4D electron microscopy. Proc. Natl. Acad. Sci. U.S.A. 110:2822-2827.
34. Maranto, A.R. 1982. Neuronal mapping: A photooxidation reaction makes Lucifer yellow useful for electron microscopy. Science 217:953-955.
35. McDonald, K.L. 2009. A review of high-pressure freezing preparation techniques for correlative light and electron microscopy of the same cells and tissues. J. Microsc. 235:273-281.
36. McIntosh, R., Nicastro, D., and Mastronarde, D. 2005. New views of cells in 3D: An introduction to electron tomography. Trends Cell Biol. 15:43-51.
37. Modla, S., Mendonca, J., Czymmek, K.J., and Akins, R.E. 2010. Identification of neuromuscular junctions by correlative confocal and transmission electronmicroscopy. J. Neurosci. Methods 191:158-165.
38. Mooney, P., Contarato, D., Denes, P., Gubbens, A., Lee, B., Lent, M., and Agard, D. 2011. A highspeed electron-counting direct detection camera for TEM. Microsc. Microanal. 17:1004-1005.
39. Moran, D.T. and Rowley, J.C. 1987. Biological specimen preparation for correlative light and electron microscopy. In Correlative Microscopy in Biology: Instrumentation and Methods (M. Hayat, ed.) pp. 2-22. Academic Press, Orlando, Fla.
40. Morphew, M.K. 2007. 3D immunolocalizationwith plastic sections. Methods Cell Biol. 79:493.
41. Nagayama, K. 2011. Phase plate electron microscopy. In Supramolecular Structure and Function 10 (J. Brnjas-Kraljević and G. Pifat- Mrzljak, eds.) pp. 101-114. Springer, The Netherlands.
42. Nisman, R., Dellaire, G., Ren, Y., Li, R., and Bazett- Jones, D.P. 2004. Application of quantum dots as probes for correlative fluorescence, conventional, and energy-filtered transmission electron microscopy. J. Histochem. Cytochem. 52:13-18.
43. Pereira, C.N., Daleprane, B., Barbosa, P.F., Moreira, A.N., and de Magalhães, C.S. 2014. Qualitative evaluation of scanning electron microscopy methods in a study of the resin cement/dentine adhesive interface. Microsc. Microanal. 20:268-275.
44. Powell, R.D., Halsey, C.M., and Hainfeld, J.F. 1998. Combined fluorescent and gold immunoprobes: reagents and methods for correlative light and electron microscopy. Microsc. Res. Techn. 42:2-12.
45. Rigort, A., Bäuerlein, F.J., Leis, A., Gruska, M., Hoffmann, C., Laugks, T., Böhm, U., Eibauer, M., Gnaegi, H., and Baumeister, W. 2010. Micromachining tools and correlative approaches for cellular cryo-electron tomography. J. Struct. Biol. 172:169-179.
46. Robinson, J.M. and Takizawa, T. 2009. Correlative fluorescence and electronmicroscopy in tissues: immunocytochemistry. J. Microsc. 235:259-272.
47. Robinson, J.M., Takizawa, T., and Vandré, D.D. 2000a. Applications of gold cluster compounds in immunocytochemistry and correlative microscopy: comparisonwith colloidal gold. J. Microsc. 199:163-179.
48. Robinson, J.M., Takizawa, T., and Vandré, D.D. 2000b. Enhanced labeling efficiency using ultrasmall immunogold probes: Immunocytochemistry. J. Histochem. Cytochem. 48:487-492.
49. Robinson, J.M., Takizawa, T., Pombo, A., and Cook, P.R. 2001. Correlative fluorescence and electron microscopy on ultrathin cryosections: Bridging the resolution gap. J. Histochem. Cytochem. 49:803-808.
50. Rust, M.J., Bates, M., and Zhuang, X. 2006. Subdiffraction- limit imaging by stochastic optical reconstruction microscopy (STORM). Nat. Methods 3:793-796.
51. Schmued, L.C. and Snavely, L.F. 1993. Photoconversion and electron microscopic localization of the fluorescent axon tracer fluoro-ruby (rhodamine-dextran-amine). J. Histochem. Cytochem. 41:777-782.
52. Schnell, U., Dijk, F., Sjollema, K.A., andGiepmans, B.N.G. 2012. Immunolabeling artifacts and the need for live-cell imaging. Nat. Methods 9:152-158.
53. Schultz, C. 2009. Fluorescent revelations. Chem. Biol. 16:107-111.
54. Schwartz, C.L., Sarbash, V.I., Ataullakhanov, F.I., McIntosh, J.R., and Nicastro, D. 2007. Cryofluorescence microscopy facilitates correlations between light and cryo-electron microscopy and reduces the rate of photobleaching. J. Microsc. 227:98-109.
55. Schwarz, H. and Humbel, B.M. 2007. Correlative light and electron microscopy using immunolabeled resin sections. ElectronMicrosc. 369:229-256.
56. Shu, X., Lev-Ram, V., Deerinck, T.J., Qi, Y., Ramko, E.B., Davidson, M.W., Jin, Y., Ellisman, M.H., and Tsien, R.Y. 2011. A genetically encoded tag for correlated light and electron microscopy of intact cells, tissues, and organisms. PLoS Biol. 9:e1001041.
57. Sims, P.A. and Hardin, J. 2004. Visualizing green fluorescent protein and fluorescence associated with a gold conjugate in thin sections with correlative confocal and electron microscopy. Microsc. Microanal. 10:156-157.
58. Sosinsky, G.E., Gaietta, G.M., Hand, G., Deerinck, T.J., Han, A., Mackey, M., Adams, S.R., Bouwer, J., Tsien, R.Y., and Ellisman, M.H. 2003. Tetracysteine genetic tags complexed with biarsenical ligands as a tool for investigating gap junction structure and dynamics. Cell Comm. Adhesion 10:181-186.
59. Sosinsky, G.E., Giepmans, B.N., Deerinck, T.J., Gaietta, G.M., and Ellisman, M.H. 2007. Markers for correlated light and electron microscopy. Methods Cell Biol. 79:575-591.
60. Sprague, B.L., Pego, R.L., Stavreva, D.A., and Mc- Nally, J.G. 2004. Analysis of binding reactions by fluorescence recovery after photobleaching. Biophys. J. 86:3473-3495.
61. Stirling, J.W. 1990. Immuno- and affinity probes for electron microscopy: A review of labeling and preparation techniques. J. Histochem. Cytochem. 38:145-157.
62. Studer, D., Humbel, B., and Chiquet, M. 2008. Electron microscopy of high pressure frozen samples: Bridging the gap between cellular ultrastructure and atomic resolution. Histochem. Cell Biol. 130:877-889.
63. Takizawa, T. and Robinson, J.M. 2012. Correlative fluorescence and transmission electron microscopy in tissues. Methods Cell Biol. 111:37-57.
64. Valentijn, J., van Driel, L., Agronskaia, A., Knoops, K., Koning, R., Barcena, M., Gerritsen, H., and Koster, A. 2008. Novel methods for cryofluorescence microscopy permitting correlative cryo-electron microscopy. Microsc. Microanal. 14:1314-1315.
65. Volkenandt, T., Müller, E., and Gerthsen, D. 2014. Sample thickness determination by scanning transmission electron microscopy at low electron energies. Microsc. Microanal. 20:111-123.
66. Von Bartheld, C.S., Cunningham, D.E., and Rubel, E.W. 1990. Neuronal tracing with DiI: Decalcification, cryosectioning, and photoconversion for light and electron microscopic analysis. J. Histochem. Cytochem. 38:725-733.
67. Wacker, I. and Schroeder, R.R. 2013. Array tomography. J. Microsc. 252:93-99.
68. Watanabe, S., Punge, A., Hollopeter, G., Willig, K.I., Hobson, R.J., Davis, M.W., Hell, S.W., and Jorgensen, E.M. 2011. Protein localization in electron micrographs using fluorescence nanoscopy. Nat. Methods 8:80-84.
69. Webster, P., Schwarz, H., and Griffiths, G. 2008. Preparation of cells and tissues for immuno EM. Methods Cell Biol. 88:45-58.
70. Westphal, V., Rizzoli, S.O., Lauterbach, M.A., Kamin, D., Jahn, R., and Hell, S.W. 2008.Videorate far-field optical nanoscopy dissects synaptic vesicle movement. Science 320:246-249.
71. Wouters, H. and Ploem, J.S. 1987. Light and scanning electron microscopy in a combined instrument. In Correlative Microscopy in Biology: Instrumentation and Methods (M. Hayat, ed.) pp. 23-38. Academic Press, Orlando, Fla.
72. Zaidi, M.R., Hornyak, T.J., and Merlino, G. 2011. A genetically engineered mouse model with inducible GFP expression in melanocytes. Pigment Cell Melanoma Res. 24:393-394.
73. Zhang, P. 2013. Correlative cryo-electron tomography and optical microscopy of cells. Curr. Opin. Struct. Biol. 23:763-770.
74. Zhao, G., Perilla, J.R., Yufenyuy, E.L., Meng, X., Chen, B., Ning, J., Ahn, J., Gronenborn, A.M., Schulten, K., Aiken, C., and Zhang, P. 2013. Mature HIV-1 capsid structure by cryo-electron microscopy and all-atom molecular dynamics. Nature 497:643-646.