A review of high-pressure freezing preparation techniques for correlative light and electron microscopy of the same cells and tissues

Journal of Microscopy

Volumn 235, Issue 3, 2009, Pages 273-281

  McDonald, K L ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Biological EM; Correlative LM EM; Freeze substitution; High pressure freezing

Indexed keywords

ELECTRON MICROSCOPES; FREEZING; REFRIGERATORS; SPECIMEN PREPARATION;

BIOLOGICAL EM; CORRELATIVE LM-EM; FREEZE SUBSTITUTION; HIGH PRESSURE; HIGH-PRESSURE FREEZING; HIGHER TIME RESOLUTION; LIGHT MICROSCOPES; LIVE CELL; PREPARATION TECHNIQUE; TRANSFER SYSTEMS;

ELECTRON MICROSCOPY;

GREEN FLUORESCENT PROTEIN;

ARTICLE; CAENORHABDITIS ELEGANS; CELL ULTRASTRUCTURE; DROSOPHILA; ELECTRON MICROSCOPY; EMBRYO CULTURE; FREEZING; HUMAN; MICROTUBULE; MITOSIS SPINDLE; NONHUMAN; PRESSURE; PRIORITY JOURNAL; TRANSMISSION ELECTRON MICROSCOPY;

EID: 69249144515     PISSN: 00222720     EISSN: 13652818     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2009.03218.x     Document Type: Article

References (23)

1. Crang, R.F.E. Klomparens, K.L. (1988) Artifacts in Biological Electron Microscopy (ed. by R.F.E. Crang & K.L. Klomperens pp. xii + 233. Plenum Press, New York.
2. Echlin, P. (1992) Low-Temperature Microscopy and Analysis. Plenum Press, New York.
3. Gilkey, J.C. Staehelin, L.A. (1986) Advances in ultrarapid freezing for the preservation of cellular ultrastructure. J. Electron Microsc. Tech. 3, 177 210.
4. Gruska, M., Medalia, O., Baumeister, W. Leis, A. 2008. Electron tomography of vitreous sections from cultured mammalian cells. J. Struct. Biol. 161, 384 392.
5. Hayat, M.A. (1987) Correlative Microscopy in Biology. Instrumentation and Methods. Academic Press, Orlando.
6. Humbel, B. (2009) Freeze substitution. Handbook of Cryo-Preparation Methods for Electron Microscopy (ed. by A. Cavalier, D. Spehner & B. Humbel pp. 319 341. CRC Press, Boca Raton, Florida.
7. Kaech, A. (2009) BAL-TEC HPM 010 High-pressure freezing machine. Handbook of Cryo-Preparation Methods for Electron Microscopy (ed. by A. Cavalier, D. Spehner & B. Humbel pp. 101 128. CRC Press, Boca Raton, Florida.
8. Kellenberger, E. (1987) The response of biological macromolecules and supramolecular structures to the physics of specimen cryopreparation. Cryotechniques in Biological Electron Microscopy (ed. by R.A. Steinbrecht & K. Zierold pp. 35 63. Springer-Verlag, Berlin.
9. Kellenberger, E., Johansen, R., Maeder, M., Bohrmann, B., Stauffer, E. Villiger, E. (1992) Artefacts and morphological changes during chemical fixation. J. Microsc. 168 : 181 201.
10. McDonald, K. (1994). Electron microscopy and EM immunocytochemistry. Drosophila melanogaster: practical uses in cell and molecular biology (ed. by L.S.B. Goldstein & E. Fyrberg Meth. Cell Biol. 44, 411 444.
11. McDonald, K.L. (1999) High pressure freezing for preservation of high resolution fine structure and antigenicity for immunolabeling. Methods Mol. Biol. 117, 77 97.
12. McDonald, K.L., Morphew M., Verkade P. Mueller-Reichert T. (2007) Recent advances in high pressure freezing. Electron Microscopy: Methods and Protocols, 2nd edn, Meth. Molec. Biol. 369 (ed. by J. Kuo pp. 143 173. Humana Press Inc., Totowa, New Jersey.
13. Müller-Reichert, T., O'Toole, E.T., Hohenberg, H. McDonald, K.L. (2003) Cryoimmobilization and three-dimensional visualization of C. elegans ultrastructure. J. Microsc. 212, 71 80.
14. Müller-Reichert, T., Srayko, M., Hyman, A.A., O'Toole, E. McDonald K. (2007) Correlative light and electron microscopy of early Caenorhabditis elegans embryos in mitosis. Meth. Cell Biol. 79, 101 119.
15. Pelletier, L., O'Toole, E., Schwager, A., Hyman, A.A. Mueller-Reichert T. (2006) Centriole assembly in Caenorhabditis elegans. Nature 44, 619 623.
16. Schwarz, H. Humbel, B 2007) Correlative light and electron microscopy using immunolabeled resin sections. Electron Microscopy: Methods and Protocols, 2nd edn, Meth. Molec. Biol. 369 (ed. by J. Kuo pp. 229 256. Humana Press Inc., Totowa, New Jersey.
17. Schwarz, H. Humbel, B. (2009) Correlative light and electron microscopy. Handbook of Cryo-Preparation Methods for Electron Microscopy (ed. by A. Cavalier, D. Spehner & B. Humbel pp. 537 565. CRC Press, Boca Raton, Florida.
18. Schwartz, C., Sarbash, V.I., Ataullakhanov, F.I., McIntosh, J.R. Nicastro, D. (2007) Cryo-fluorescence microscopy facilitates correlations between light and cryo-electron microscopy and reduces the rate of photobleaching. J. Microsc. 227, 98 109.
19. Sims, P.A. Hardin, J.D. (2007) Fluorescence-integrated transmission electron microscopy images. Electron Microscopy: Methods and Protocols, 2nd edn, Meth. Molec. Biol. 369 (ed. by J. Kuo pp. 291 308. Humana Press Inc., Totowa, New Jersey.
20. Srayko, M., O'Toole, E.T., Hyman, A.A. Mueller-Reichert, T. (2006) Katanin disrupts the microtubule lattice and increases polymer number in C. elegans meiosis. Curr. Biol. 16, 1944 1949.
21. Studer, D., Graber, W., Al-Amoudi, A. Eggli, P. (2001) A new approach for cryofixation by high-pressure freezing. J. Microsc. Oxford 203, 285 294.
22. Vanhecke, D., & Studer, D. (2009) High-pressure freezing LEICA EMPACT. Handbook of Cryo-Preparation Methods for Electron Microscopy (ed. by A. Cavalier, D. Spehner & B. Humbel pp. 129 156. CRC Press, Boca Raton, Florida.
23. Verkade, P. (2008) Moving EM: the rapid transfer system as a new tool for correlative light and electron microscopy and high throughput for high-pressure freezing. J. Microsc. 230, 317 328.