Chapter 3 Preparation of Cells and Tissues for Immuno EM

Methods in Cell Biology

Volumn 88, Issue , 2008, Pages 45-58

  Webster, Paul ^a   Schwarz, Heinz ^b   Griffiths, Gareth ^a  

^a Cell Biology and Biophysics Unit   (Germany)

^b MAX PLANCK INSTITUTE FOR DEVELOPMENTAL BIOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CROSS LINKING REAGENT;

ANIMAL; CELL; CRYOPRESERVATION; CYTOLOGY; FREEZE DRYING; FROZEN SECTION; HISTOLOGY; HUMAN; IMMUNOHISTOCHEMISTRY; LABORATORY DIAGNOSIS; METABOLISM; METHODOLOGY; REVIEW; TRANSMISSION ELECTRON MICROSCOPY; ULTRAMICROTOMY; ULTRASTRUCTURE;

ANIMALS; CELLS; CROSS-LINKING REAGENTS; CRYOPRESERVATION; CRYOULTRAMICROTOMY; FREEZE SUBSTITUTION; FROZEN SECTIONS; HUMANS; IMMUNOHISTOCHEMISTRY; MICROSCOPY, ELECTRON, TRANSMISSION; SPECIMEN HANDLING; TISSUE EMBEDDING;

EID: 46449139222     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0091-679X(08)00403-2     Document Type: Review

References (71)

1. Al-Amoudi A., Chang J.J., Leforestier A., McDowall A., Salamin L.M., Norlen L.P., Richter K., Blanc N.S., Studer D., and Dubochet J. Cryo-electron microscopy of vitreous sections. Embo J. 23 (2004) 3583-3588
2. Al-Amoudi A., Dubochet J., and Studer D. Amorphous solid water produced by cryosectioning of crystalline ice at 113 K. J. Microsc. 207 (2002) 146-153
3. Al-Amoudi A., Norlen L.P., and Dubochet J. Cryo-electron microscopy of vitreous sections of native biological cells and tissues. J. Struct. Biol. 148 (2004) 131-135
4. Al-Amoudi A., Studer D., and Dubochet J. Cutting artefacts and cutting process in vitreous sections for cryo-electron microscopy. J. Struct. Biol. 150 (2005) 109-121
5. Altman L.G., Schneider B.G., and Papermaster D.S. Rapid embedding of tissues in Lowicryl K4M for immunoelectron microscopy. J. Histochem. Cytochem. 32 (1984) 1217-1223
6. Armbruster B.L., Carlemalm E., Chiovetti R., Garavito R.M., Hobot J.A., Kellenberger E., and Villiger W. Specimen preparation for electron microscopy using low temperature embedding resins. J. Microsc. 126 (1982) 77-85
7. Baumeister W., Grimm R., and Walz J. Electron tomography of molecules and cells. Trends Cell. Biol. 9 (1999) 81-85
8. Bayer M.E., Carlemalm E., and Kellenberger E. Capsule of Escherichia coli K29: Ultrastructural preservation and immunoelectron microscopy. J. Bacteriol. 162 (1985) 985-991
9. Beck M., Forster F., Ecke M., Plitzko J.M., Melchior F., Gerisch G., Baumeister W., and Medalia O. Nuclear pore complex structure and dynamics revealed by cryoelectron tomography. Science 306 (2004) 1387-1390
10. Biel S.S., Kawaschinski K., Wittern K.P., Hintze U., and Wepf R. From tissue to cellular ultrastructure: Closing the gap between micro- and nanostructural imaging. J. Microsc. 212 (2003) 91-99
11. Bohrmann B., and Kellenberger E. Cryosubstitution of frozen biological specimens in electron microscopy: Use and application as an alternative to chemical fixation. Micron 32 (2001) 11-19
12. Braet F., Bomans P.H., Wisse E., and Frederik P.M. The observation of intact hepatic endothelial cells by cryo-electron microscopy. J. Microsc. 212 (2003) 175-185
13. Carlemalm E., Villiger W., Hobot J.A., Acetarin J.D., and Kellenberger E. Low temperature embedding with Lowicryl resins: Two new formulations and some applications. J. Microsc. 140 (1985) 55-63
14. Cyrklaff M., Risco C., Fernandez J.J., Jimenez M.V., Esteban M., Baumeister W., and Carrascosa J.L. Cryo-electron tomography of vaccinia virus. Proc. Natl. Acad. Sci. USA 102 (2005) 2772-2777
15. Dubochet J., Adrian M., Chang J.J., Homo J.C., Lepault J., McDowall A.W., and Schultz P. Cryo-electron microscopy of vitrified specimens. Q. Rev. Biophys. 21 (1988) 129-228
16. Gaietta G., Deerinck T.J., Adams S.R., Bouwer J., Tour O., Laird D.W., Sosinsky G.E., Tsien R.Y., and Ellisman M.H. Multicolor and electron microscopic imaging of connexin trafficking. Science 296 (2002) 503-507
17. Gaietta G.M., Giepmans B.N., Deerinck T.J., Smith W.B., Ngan L., Llopis J., Adams S.R., Tsien R.Y., and Ellisman M.H. Golgi twins in late mitosis revealed by genetically encoded tags for live cell imaging and correlated electron microscopy. Proc. Natl. Acad. Sci. USA 103 (2006) 17777-17782
18. Giddings T.H. Freeze-substitution protocols for improved visualization of membranes in high-pressure frozen samples. J. Microsc. 212 (2003) 53-61
19. Gilkey J.C., and Staehelin L.A. Advances in ultrarapid freezing for the preservation of cellular ultrastructure. J. Electron Microsc. Tech. 3 (1986) 177-210
20. Gocht A. Use of LR white resin for post-embedding immunolabelling of brain tissue. Acta Anat. (Basel) 145 (1992) 327-339
21. Griffiths G. Fine Structure Immunocytochemistry (1993), Springer, Berlin
22. Griffiths G., McDowall A., Back R., and Dubochet J. On the preparation of cryosections for immunocytochemistry. J. Ultrastruct. Res. 89 (1984) 65-78
23. Griffiths G., Simons K., Warren G., and Tokuyasu K.T. Immunoelectron microscopy using thin, frozen sections: Application to studies of the intracellular transport of Semliki Forest virus spike glycoproteins. Methods Enzymol. 96 (1983) 466-485
24. Hagler H.K. Ultramicrotome for biological electron microscopy. In: Kuo J. (Ed). Methods in Molecular Biology. Electron microscopy: Methods and Protocols Vol. 369 (2007), Humana Press, Totowa, NJ 67-106
25. Han B., and Bischof J.C. Direct cell injury associated with eutectic crystallization during freezing. Cryobiology 48 (2004) 8-21
26. Hess M.W. Cryopreparation methodology for plant cell biology. In: McIntosh J.R. (Ed). Cellular Electron Microscopy (2007), Elsevier, Oxford, UK
27. Himmelhoch S. A negative contrast stain for ultra-thin frozen sections. Microsc. Res. Tech. 29 (1994) 23-28
28. Hobot J.A. Lowicryls and low temperature embedding for colloidal gold methods. In: Hayat M.A. (Ed). Colloidal gold: Principles, methods, and applications Vol. 2 (1989), Academic Press, San Diego, CA 75
29. Huisken J., Swoger J., Del Bene F., Wittbrodt J., and Stelzer E.H. Optical sectioning deep inside live embryos by selective plane illumination microscopy. Science 305 (2004) 1007-1009
30. Humbel B.M., and Schwarz H. Freeze substitution for immunocytochemistry. In: Verkleij A.J., Leunissen J.L.M., and Insler V. (Eds). Immuno-gold labeling in cell biology (1989), CRC Press, Boca Raton, FL 114-134
31. Humbel B.M., and Schwarz H. Freeze-substitution for immunochemistry. In: Verkleij A.J., and Leunissen J.L.M. (Eds). Immuno-gold labelling in cell biology (1989), CRC Press, Boca Raton, FL 115
32. Kellenberger E. The potential of cryofixation and freeze-substitution: Observations and theoretical considerations. J. Microsc. 161 (1991) 183-203
33. Keller G.A., Tokuyasu K.T., Dutton A.H., and Singer S.J. An improved procedure for immunoelectron microscopy: Ultrathin plastic embedding of immunolabeled ultrathin frozen sections. Proc. Natl. Acad. Sci. USA 81 (1984) 5744-5747
34. Kurner J., Medalia O., Linaroudis A.A., and Baumeister W. New insights into the structural organization of eukaryotic and prokaryotic cytoskeletons using cryo-electron tomography. Exp. Cell Res. 301 (2004) 38-42
35. Lucocq J.M., and Roth J. Applications of immunocolloids in light microscopy. III. Demonstration of antigenic and lectin-binding sites in semithin resin sections. J. Histochem. Cytochem. 32 (1984) 1075-1083
36. McDonald K. High-pressure freezing for preservation of high resolution fine structure and antigenicity for immunolabeling. Methods Mol. Biol. 117 (1999) 77-97
37. McDonald K.L., Morphew M., Verkade P., and Müller-Reichert T. Recent advances in high-pressure freezing. In: Kuo J. (Ed). Methods in Molecular Biology. Electron microscopy: Methods and protocols Vol. 369 (2007), Humana Press, Totowa, NJ 143-173
38. McIntosh R., Nicastro D., and Mastronarde D. New views of cells in 3D: An introduction to electron tomography. Trends Cell Biol. 15 (2005) 43-51
39. Medalia O., Beck M., Ecke M., Weber I., Neujahr R., Baumeister W., and Gerisch G. Organization of actin networks in intact filopodia. Curr. Biol. 17 (2007) 79-84
40. Medalia O., Weber I., Frangakis A.S., Nicastro D., Gerisch G., and Baumeister W. Macromolecular architecture in eukaryotic cells visualized by cryoelectron tomography. Science 298 (2002) 1209-1213
41. Moor H. Theory and practice of high pressure freezing. In: Steinbrecht R.A., and Zierold K. (Eds). Cryotechniques in biological electron microscopy (1987), Springer, Berlin/Heidelberg 175-191
42. Mori M., Ishikawa G., Takeshita T., Goto T., Robinson J.M., and Takizawa T. Ultrahigh-resolution immunofluorescence microscopy using ultrathin cryosections: Subcellular distribution of caveolin-1alpha and CD31 in human placental endothelial cells. J. Electron Microsc. (Tokyo) 55 (2006) 107-112
43. Muller-Reichert T., Srayko M., Hyman A., O'Toole E.T., and McDonald K. Correlative light and electron microscopy of early Caenorhabditis elegans embryos in mitosis. Methods Cell. Biol. 79 (2007) 101-119
44. Newman G.R., and Hobot J.A. Modern acrylics for post-embedding immunostaining techniques. J. Histochem. Cytochem. 35 (1987) 971-981
45. Oprins A., Geuze H.J., and Slot J.W. Cryosubstitution dehydration of aldehyde-fixed tissue: A favorable approach to quantitative immunocytochemistry. J. Histochem. Cytochem. 42 (1994) 497-503
46. Osamura R.Y., Itoh Y., and Matsuno A. Applications of plastic embedding to electron microscopic immunocytochemistry and in situ hybridization in observations of production and secretion of peptide hormones. J. Histochem. Cytochem. 48 (2000) 885-891
47. Robertson D., Monaghan P., Clarke C., and Atherton A.J. An appraisal of low-temperature embedding by progressive lowering of temperature into Lowicryl HM20 for immunocytochemical studies. J. Microsc. 168 (1992) 85-100
48. Robinson J.M., Takizawa T., Pombo A., and Cook P.R. Correlative fluorescence and electron microscopy on ultrathin cryosections: Bridging the resolution gap. J. Histochem. Cytochem. 49 (2001) 803-808
49. Roos N., Cyrklaff M., Cudmore S., Blasco R., Krijnse-Locker J., and Griffiths G. A novel immunogold cryoelectron microscopic approach to investigate the structure of the intracellular and extracellular forms of vaccinia virus. EMBO J. 15 (1996) 2343-2355
50. Roos N., and Morgan J.A. Cryopreparation of thin biological specimens for electron microscopy: Methods and applications (1990), Oxford Scientific Publications
51. Saibil H.R. Conformational changes studied by cryo-electron microscopy. Nat. Struct. Biol. 7 (2000) 711-714
52. Saibil H.R. Macromolecular structure determination by cryo-electron microscopy. Acta Crystallogr. D 56 (2000) 1215-1222
53. Schwarz H., Hohenberg H., and Humbel B.M. Freeze-substitution in virus research: A preview. In: Hyatt A.D., and Eaton B.T. (Eds). Immuno-gold electron microscopy (1993), CRC Press, Boca-Raton, FL 349-376
54. Schwarz H., and Humbel B.M. Correlative light and electron microscopy using immunolabeled resin sections. In: Kuo J. (Ed). Methods in Molecular Biology. Electron microscopy: Methods and Protocols Vol. 369 (2007), Humana Press, Totowa, NJ 229-256
55. Sosinsky G.E., Giepmans B.N., Deerinck T.J., Gaietta G.M., and Ellisman M.H. Markers for correlated light and electron microscopy. In: McIntosh J.R. (Ed). Cellular Electron Microscopy Vol. 79 (2007), Elsevier 575-591
56. Steven A.C., and Aebi U. The next ice age: Cryo-electron tomography of intact cells. Trends Cell Biol. 13 (2003) 107-110
57. Stierhof Y.D., Schwarz H., and Frank H. Transverse sectioning of plastic-embedded immunolabeled cryosections: morphology and permeability to protein A-colloidal gold complexes. J. Ultrastruct. Mol. Struct. Res. 97 (1986) 187-196
58. Takizawa T., Anderson C.L., and Robinson J.M. A new method to enhance contrast of ultrathin cryosections for immunoelectron microscopy. J. Histochem. Cytochem. 51 (2003) 31-39
59. Takizawa T., and Robinson J.M. Correlative microscopy of ultrathin cryosections is a powerful tool for placental research. Placenta 24 (2003) 557-565
60. Takizawa T., and Robinson J.M. Ultrathin cryosections: An important tool for immunofluorescence and correlative microscopy. J. Histochem. Cytochem. 51 (2003) 707-714
61. Takizawa T., and Robinson J.M. Thin is better! Ultrathin cryosection immunocytochemistry. J. Nippon. Med. Sch. 71 (2004) 306-307
62. Takizawa T., and Robinson J.M. Correlative microscopy of ultrathin cryosections in placental research. Methods Mol. Med. 121 (2006) 351-369
63. Tokuyasu K.T. Immunochemistry on ultrathin frozen sections. Histochem J. 12 (1980) 381-403
64. Tokuyasu K.T. Application of cryoultramicrotomy to immunocytochemistry. J. Microsc. 143 (1986) 139-149
65. Tokuyasu K.T. Use of poly(vinylpyrrolidone) and poly(vinyl alcohol) for cryoultramicrotomy. Histochem J. 21 (1989) 163-171
66. van Genderen I.L., van Meer G., Slot J.W., Geuze H.J., and Voorhout W.F. Subcellular localization of Forssman glycolipid in epithelial MDCK cells by immuno-electronmicroscopy after freeze-substitution. J. Cell. Biol. 115 (1991) 1009-1019
67. Waller R.F., Keeling P.J., Donald R.G., Striepen B., Handman E., Lang-Unnasch N., Cowman A.F., Besra G.S., Roos D.S., and McFadden G.I. Nuclear-encoded proteins target to the plastid in Toxoplasma gondii and Plasmodium falciparum. Proc. Natl. Acad. Sci. USA 95 (1998) 12352-12357
68. Walther P., and Ziegler A. Freeze substitution of high-pressure frozen samples: The visibility of biological membranes is improved when the substitution medium contains water. J. Microsc. 208 (2002) 3-10
69. Warhol M.J., Lucocq J.M., Carlemalm E., and Roth J. Ultrastructural localization of keratin proteins in human skin using low-temperature embedding and the protein A-gold technique. J. Invest. Dermatol. 84 (1985) 69-72
70. Webster P. Microwave-assisted processing and embedding for transmission electron microscopy. In: Kuo J. (Ed). Methods in Molecular Biology. Electron microscopy: Methods and Protocols Vol. 369 (2007), Humana Press, Totowa, NJ 47-65
71. Webster P., and Webster A. Cryosectioning fixed and cryoprotected biological material for immunocytochemistry. In: Kuo J. (Ed). Methods in Molecular Biology: Electron microscopy: Methods and Protocols Vol. 369 (2007), Humana Press, Totowa, NJ 257-289