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Volumn 111, Issue , 2012, Pages 75-93

Labeling of Ultrathin Resin Sections for Correlative Light and Electron Microscopy

Author keywords

Correlative light and electron microscopy (CLEM); Fluorescence; Lowicryl K4M; PLT embedding; Protein A gold; Retina; Testis

Indexed keywords


EID: 84864386796     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/B978-0-12-416026-2.00005-4     Document Type: Chapter
Times cited : (27)

References (61)
  • 2
    • 70449119955 scopus 로고    scopus 로고
    • Studying intracellular transport using high-pressure freezing and correlative light electron microscopy
    • Brown E., Mantell J., Carter D.A., Tilly G., Verkade P. Studying intracellular transport using high-pressure freezing and correlative light electron microscopy. Seminars in Cell & Developmental Biology 2009, 20:910-919.
    • (2009) Seminars in Cell & Developmental Biology , vol.20 , pp. 910-919
    • Brown, E.1    Mantell, J.2    Carter, D.A.3    Tilly, G.4    Verkade, P.5
  • 3
    • 0020327085 scopus 로고
    • Resin development for electron microscopy and an analysis of embedding at low temperature
    • Carlemalm E., Garavito R.M., Villiger W. Resin development for electron microscopy and an analysis of embedding at low temperature. Journal of Microscopy 1982, 126:123-143.
    • (1982) Journal of Microscopy , vol.126 , pp. 123-143
    • Carlemalm, E.1    Garavito, R.M.2    Villiger, W.3
  • 4
    • 84884763787 scopus 로고    scopus 로고
    • Progressive lowering of temperature for immunolabeling and in situ hybridization
    • CRC Press, Boca Raton, FL, A. Cavalier, D. Spehner, B.M. Humbel (Eds.)
    • Cavalier A., Spehner D. Progressive lowering of temperature for immunolabeling and in situ hybridization. Hand book of cryo-preparation methods for electron microscopy 2009, 433-465. CRC Press, Boca Raton, FL. A. Cavalier, D. Spehner, B.M. Humbel (Eds.).
    • (2009) Hand book of cryo-preparation methods for electron microscopy , pp. 433-465
    • Cavalier, A.1    Spehner, D.2
  • 5
    • 23444431611 scopus 로고
    • Green fluorescent protein as a marker for gene expression
    • Chalfie M., Tu Y., Euskirchen G., Ward W.W., Prasher D.C. Green fluorescent protein as a marker for gene expression. Science 1994, 262:802-805.
    • (1994) Science , vol.262 , pp. 802-805
    • Chalfie, M.1    Tu, Y.2    Euskirchen, G.3    Ward, W.W.4    Prasher, D.C.5
  • 6
    • 2942678703 scopus 로고    scopus 로고
    • Knock-in human rhodopsin-GFP fusions as mouse models for human disease and targets for gene therapy
    • Chan F., Bradley A., Wensel T.G., Wilson J.H. Knock-in human rhodopsin-GFP fusions as mouse models for human disease and targets for gene therapy. PNAS 2004, 101:9109-9114.
    • (2004) PNAS , vol.101 , pp. 9109-9114
    • Chan, F.1    Bradley, A.2    Wensel, T.G.3    Wilson, J.H.4
  • 7
    • 73349108505 scopus 로고    scopus 로고
    • Advanced correlative light/electron microscopy: current methods and new developments using Tokuyasu cryosections
    • Cortese K., Diaspro A., Tacchetti C. Advanced correlative light/electron microscopy: current methods and new developments using Tokuyasu cryosections. Journal of Histochemistry Cytochemistry 2009, 57:1103-1112.
    • (2009) Journal of Histochemistry Cytochemistry , vol.57 , pp. 1103-1112
    • Cortese, K.1    Diaspro, A.2    Tacchetti, C.3
  • 9
  • 11
    • 33845196026 scopus 로고    scopus 로고
    • Golgi twins in late mitosis revealed by genetically encoded tags for live cell imaging and correlated electron microscopy
    • Gaietta G.M., Giepmans B.N.G., Deerinck T.J., Smith W.B., Ngan L., Llopis J., et al. Golgi twins in late mitosis revealed by genetically encoded tags for live cell imaging and correlated electron microscopy. PNAS 2006, 103:17777-17782.
    • (2006) PNAS , vol.103 , pp. 17777-17782
    • Gaietta, G.M.1    Giepmans, B.N.G.2    Deerinck, T.J.3    Smith, W.B.4    Ngan, L.5    Llopis, J.6
  • 12
    • 33645798851 scopus 로고    scopus 로고
    • The fluorescence toolbox for assessing protein location and function
    • Giepmans B.N., Adams S.R., Ellisman M.H., Tsien R.Y. The fluorescence toolbox for assessing protein location and function. Science 2006, 312:217-224.
    • (2006) Science , vol.312 , pp. 217-224
    • Giepmans, B.N.1    Adams, S.R.2    Ellisman, M.H.3    Tsien, R.Y.4
  • 13
    • 48749110649 scopus 로고    scopus 로고
    • Bridging fluorescence microscopy and electron microscopy
    • Giepmans B.N. Bridging fluorescence microscopy and electron microscopy. Histochemistry and Cell Biology 2008, 130:211-217.
    • (2008) Histochemistry and Cell Biology , vol.130 , pp. 211-217
    • Giepmans, B.N.1
  • 16
    • 34249945258 scopus 로고    scopus 로고
    • Far-field optical nanoscopy
    • Hell S.W. Far-field optical nanoscopy. Science 2007, 316:1153-1158.
    • (2007) Science , vol.316 , pp. 1153-1158
    • Hell, S.W.1
  • 17
    • 58149236663 scopus 로고    scopus 로고
    • Microscopy and its focal switch
    • Hell S.W. Microscopy and its focal switch. Nature Methods 2009, 6:24-32.
    • (2009) Nature Methods , vol.6 , pp. 24-32
    • Hell, S.W.1
  • 18
    • 0029935328 scopus 로고    scopus 로고
    • Ependymins: meningeal-derived extracellular matrix proteins at the blood-brain barrier
    • Hoffmann W., Schwarz H. Ependymins: meningeal-derived extracellular matrix proteins at the blood-brain barrier. International Review of Cytology 1996, 165:121-158.
    • (1996) International Review of Cytology , vol.165 , pp. 121-158
    • Hoffmann, W.1    Schwarz, H.2
  • 19
    • 0002346809 scopus 로고
    • Freeze substitution for immunochemistry
    • CRC Press, Boca Raton, FL:, A.J. Verklej, J.L.M. Leunissen (Eds.)
    • Humbel B.M., Schwarz H. Freeze substitution for immunochemistry. Immuno-gold labeling in cell biology 1989, 115-134. CRC Press, Boca Raton, FL:. A.J. Verklej, J.L.M. Leunissen (Eds.).
    • (1989) Immuno-gold labeling in cell biology , pp. 115-134
    • Humbel, B.M.1    Schwarz, H.2
  • 20
    • 0026918335 scopus 로고
    • A preparation protocol for postembedding immunoelectron microscopy of Dictyostelium discoideum cells with monoclonal antibodies
    • Humbel B.M., Biegelmann E. A preparation protocol for postembedding immunoelectron microscopy of Dictyostelium discoideum cells with monoclonal antibodies. Scanning Microscopy 1992, 6:817-825.
    • (1992) Scanning Microscopy , vol.6 , pp. 817-825
    • Humbel, B.M.1    Biegelmann, E.2
  • 21
    • 0031928016 scopus 로고    scopus 로고
    • Pre-embedding immunolabeling for electron microscopy: an evaluation of permeabilization methods and markers
    • Humbel B.M., de Jong M.D., Müller W.H., Verkleij A.J. Pre-embedding immunolabeling for electron microscopy: an evaluation of permeabilization methods and markers. Microscopy Research and Technique 1998, 42:43-58.
    • (1998) Microscopy Research and Technique , vol.42 , pp. 43-58
    • Humbel, B.M.1    de Jong, M.D.2    Müller, W.H.3    Verkleij, A.J.4
  • 22
    • 69249126071 scopus 로고    scopus 로고
    • Freeze substitution
    • CRC Press, Boca Raton, FL, A. Cavalier, D. Spehner, B.M. Humbel (Eds.)
    • Humbel B.M. Freeze substitution. Hand book of cryo-preparation methods for electron microscopy 2009, 319-341. CRC Press, Boca Raton, FL. A. Cavalier, D. Spehner, B.M. Humbel (Eds.).
    • (2009) Hand book of cryo-preparation methods for electron microscopy , pp. 319-341
    • Humbel, B.M.1
  • 23
    • 13444302486 scopus 로고    scopus 로고
    • The acrosome-acroplaxome-manchette complex and the shaping of the spermatid head
    • Kierszenbaum A.L., Tres L.L. The acrosome-acroplaxome-manchette complex and the shaping of the spermatid head. Archives of Histology and Cytology 2004, 67:271-284.
    • (2004) Archives of Histology and Cytology , vol.67 , pp. 271-284
    • Kierszenbaum, A.L.1    Tres, L.L.2
  • 24
    • 77449136170 scopus 로고    scopus 로고
    • A precise and rapid mapping protocol for correlative light and electron microscopy of small invertebrate organisms
    • Kolotuev I., Schwab Y., Labouesse M. A precise and rapid mapping protocol for correlative light and electron microscopy of small invertebrate organisms. Biology of the Cell 2010, 102:121-132.
    • (2010) Biology of the Cell , vol.102 , pp. 121-132
    • Kolotuev, I.1    Schwab, Y.2    Labouesse, M.3
  • 25
    • 0029988291 scopus 로고    scopus 로고
    • Fine structural immunocytochemistry of catenins in amphibian and mammalian muscle
    • Kurth T., Schwarz H., Schneider S., Hausen P. Fine structural immunocytochemistry of catenins in amphibian and mammalian muscle. Cell and Tissue Research 1996, 286:1-12.
    • (1996) Cell and Tissue Research , vol.286 , pp. 1-12
    • Kurth, T.1    Schwarz, H.2    Schneider, S.3    Hausen, P.4
  • 27
    • 0042526009 scopus 로고    scopus 로고
    • Immunocytochemistry of the amphibian embryo - from overview to ultrastructure
    • Kurth T. Immunocytochemistry of the amphibian embryo - from overview to ultrastructure. International Journal of Developmental Biology 2003, 47:373-383.
    • (2003) International Journal of Developmental Biology , vol.47 , pp. 373-383
    • Kurth, T.1
  • 29
    • 0025254201 scopus 로고
    • A neutral pH silver development method for the visualization of 1-nanometer gold particles in pre-embedding electron microscopic immunocytochemistry
    • Lah J.J., Hayes D.M., Burry R.W. A neutral pH silver development method for the visualization of 1-nanometer gold particles in pre-embedding electron microscopic immunocytochemistry. Journal of Histochemistry and Cytochemistry 1990, 38:503-508.
    • (1990) Journal of Histochemistry and Cytochemistry , vol.38 , pp. 503-508
    • Lah, J.J.1    Hayes, D.M.2    Burry, R.W.3
  • 30
    • 0020448397 scopus 로고
    • Applications of immobilized protein A in immunochemical techniques
    • Langone J.J. Applications of immobilized protein A in immunochemical techniques. Journal of Immunological Methods 1982, 55:277-296.
    • (1982) Journal of Immunological Methods , vol.55 , pp. 277-296
    • Langone, J.J.1
  • 32
    • 69249144515 scopus 로고    scopus 로고
    • A review of high-pressure freezing preparation techniques for correlative light and electron microscopy of the same cells and tissues
    • McDonald K.L. A review of high-pressure freezing preparation techniques for correlative light and electron microscopy of the same cells and tissues. Journal of Microscopy 2009, 235:273-281.
    • (2009) Journal of Microscopy , vol.235 , pp. 273-281
    • McDonald, K.L.1
  • 33
    • 0026529270 scopus 로고
    • Redistribution and differential extraction of soluble proteins in permabilized cultured cells. Implications for immunofluorescence microscopy
    • Melan M.A., Sluder G. Redistribution and differential extraction of soluble proteins in permabilized cultured cells. Implications for immunofluorescence microscopy. Journal of Cell Science 1992, 101:731-743.
    • (1992) Journal of Cell Science , vol.101 , pp. 731-743
    • Melan, M.A.1    Sluder, G.2
  • 35
    • 63049118188 scopus 로고    scopus 로고
    • Correlation of 4Pi- and electron microscopy to study transport through single golgi stacks in living cells with super resolution
    • Perinetti G., Müller T., Spaar A., Polishchuk R., Luini A., Egner A. Correlation of 4Pi- and electron microscopy to study transport through single golgi stacks in living cells with super resolution. Traffic 2009, 10:379-391.
    • (2009) Traffic , vol.10 , pp. 379-391
    • Perinetti, G.1    Müller, T.2    Spaar, A.3    Polishchuk, R.4    Luini, A.5    Egner, A.6
  • 36
    • 0034627793 scopus 로고    scopus 로고
    • Correlative light-electron microscopy reveals the tubular-saccular ultrastructure of carriers operating between golgi apparatus and plasma membrane
    • Polishchuk R.S., Polishchuk E.V., Marra P., Alberti S., Buccione R., Luini A., et al. Correlative light-electron microscopy reveals the tubular-saccular ultrastructure of carriers operating between golgi apparatus and plasma membrane. Journal of Cell Biology 2000, 148:45-58.
    • (2000) Journal of Cell Biology , vol.148 , pp. 45-58
    • Polishchuk, R.S.1    Polishchuk, E.V.2    Marra, P.3    Alberti, S.4    Buccione, R.5    Luini, A.6
  • 38
    • 0033817766 scopus 로고    scopus 로고
    • Applications of gold cluster compounds in immunocytochemistry and correlative microscopy: Comparison with colloidal gold
    • Robinson J.M., Takizawa T., Vandre D.D. Applications of gold cluster compounds in immunocytochemistry and correlative microscopy: Comparison with colloidal gold. Journal of Microscopy 2000, 199:163-179.
    • (2000) Journal of Microscopy , vol.199 , pp. 163-179
    • Robinson, J.M.1    Takizawa, T.2    Vandre, D.D.3
  • 39
    • 69249159143 scopus 로고    scopus 로고
    • Correlative fluorescence and electron microscopy in tissues: Immunocytochemistry
    • Robinson J.M., Takizawa T. Correlative fluorescence and electron microscopy in tissues: Immunocytochemistry. Journal of Microscopy 2009, 235:259-272.
    • (2009) Journal of Microscopy , vol.235 , pp. 259-272
    • Robinson, J.M.1    Takizawa, T.2
  • 41
    • 0027182418 scopus 로고
    • Ultrastructural localization of ependymins in the endomeninx of the brain of the rainbow trout: Possible association with collagen fibrils of the extracellular matrix
    • Schwarz H., Müller-Schmid A., Hoffmann W. Ultrastructural localization of ependymins in the endomeninx of the brain of the rainbow trout: Possible association with collagen fibrils of the extracellular matrix. Cell and Tissue Research 1993, 273:417-425.
    • (1993) Cell and Tissue Research , vol.273 , pp. 417-425
    • Schwarz, H.1    Müller-Schmid, A.2    Hoffmann, W.3
  • 43
    • 0042685233 scopus 로고
    • Immunolabeling of ultrathin sections for fluorescence and electron microscopy
    • Les Editions de Physique, Les Ulis, France, B. Jouffrey, C. Colliex (Eds.)
    • Schwarz H. Immunolabeling of ultrathin sections for fluorescence and electron microscopy. Electron microscopy 1994, ICEM 13-Paris 1994, Vol. 3:255-256. Les Editions de Physique, Les Ulis, France. B. Jouffrey, C. Colliex (Eds.).
    • (1994) Electron microscopy 1994, ICEM 13-Paris , vol.3 , pp. 255-256
    • Schwarz, H.1
  • 44
    • 84864388876 scopus 로고    scopus 로고
    • Correlative immunolabeling of ultrathin sections for light and electron microscopy
    • Institute of Physics Publishing, Bristol, PA, H.A. Calderon Benavides (Ed.)
    • Schwarz H. Correlative immunolabeling of ultrathin sections for light and electron microscopy. Electron microscopy 1998, ICEM 14 1998, 865-866. Institute of Physics Publishing, Bristol, PA. H.A. Calderon Benavides (Ed.).
    • (1998) Electron microscopy 1998, ICEM 14 , pp. 865-866
    • Schwarz, H.1
  • 45
    • 34548020973 scopus 로고    scopus 로고
    • Correlative light and electron microscopy using immunolabeled resin sections
    • Schwarz H., Humbel B.M. Correlative light and electron microscopy using immunolabeled resin sections. Methods in Molecular Biology 2007, 369:229-256.
    • (2007) Methods in Molecular Biology , vol.369 , pp. 229-256
    • Schwarz, H.1    Humbel, B.M.2
  • 46
    • 69249099205 scopus 로고    scopus 로고
    • Correlative light and electron microscopy
    • CRC Press, Boca Raton, FL, A. Cavalier, D. Spehner, B.M. Humbel (Eds.)
    • Schwarz H., Humbel B.M. Correlative light and electron microscopy. Hand book of cryo-preparation methods for electron microscopy 2009, 537-565. CRC Press, Boca Raton, FL. A. Cavalier, D. Spehner, B.M. Humbel (Eds.).
    • (2009) Hand book of cryo-preparation methods for electron microscopy , pp. 537-565
    • Schwarz, H.1    Humbel, B.M.2
  • 47
    • 34548043473 scopus 로고    scopus 로고
    • Fuorescence-integrated transmission electron microscopy images: Integrating fluorescence microscopy with transmission electron microscopy
    • Sims P.A., Hardin J.D. Fuorescence-integrated transmission electron microscopy images: Integrating fluorescence microscopy with transmission electron microscopy. Methods in Molecular Biology 2007, 369:291-308.
    • (2007) Methods in Molecular Biology , vol.369 , pp. 291-308
    • Sims, P.A.1    Hardin, J.D.2
  • 48
    • 38449099776 scopus 로고    scopus 로고
    • Cryosectioning and immunolabeling
    • Slot J.W., Geuze H.J. Cryosectioning and immunolabeling. Nature Protocol 2007, 2:2480-2491.
    • (2007) Nature Protocol , vol.2 , pp. 2480-2491
    • Slot, J.W.1    Geuze, H.J.2
  • 49
    • 0026079333 scopus 로고
    • Suitability of different silver enhancement methods applied to 1 nm colloidal gold particles: An immunoelectron microscopic study
    • Stierhof Y.D., Humbel B.M., Schwarz H. Suitability of different silver enhancement methods applied to 1 nm colloidal gold particles: An immunoelectron microscopic study. Journal of Electron Microscopy Technology 1991, 17:336-343.
    • (1991) Journal of Electron Microscopy Technology , vol.17 , pp. 336-343
    • Stierhof, Y.D.1    Humbel, B.M.2    Schwarz, H.3
  • 50
    • 77957011546 scopus 로고    scopus 로고
    • Immunolabeling of ultra-thin sections with enlarged 1 nm gold or Q-dots
    • CRC Press, Boca Raton, FL, A. Cavalier, D. Spehner, B.M. Humbel (Eds.)
    • Stierhof Y.D. Immunolabeling of ultra-thin sections with enlarged 1 nm gold or Q-dots. Hand book of cryo-preparation methods for electron microscopy 2009, 587-616. CRC Press, Boca Raton, FL. A. Cavalier, D. Spehner, B.M. Humbel (Eds.).
    • (2009) Hand book of cryo-preparation methods for electron microscopy , pp. 587-616
    • Stierhof, Y.D.1
  • 52
    • 54049097431 scopus 로고    scopus 로고
    • Electron Microscopy of high pressure frozen samples: Bridging the gap between cellular ultrastructure and atomic resolution
    • Studer D., Humbel B.M., Chiquet M. Electron Microscopy of high pressure frozen samples: Bridging the gap between cellular ultrastructure and atomic resolution. Histochemistry and Cell Biology 2008, 130:877-889.
    • (2008) Histochemistry and Cell Biology , vol.130 , pp. 877-889
    • Studer, D.1    Humbel, B.M.2    Chiquet, M.3
  • 53
    • 0031695430 scopus 로고    scopus 로고
    • Correlative microscopy using FluoroNanogold on ultrathin cryosections. Proof of principle
    • Takizawa T., Suzuki K., Robinson J.M. Correlative microscopy using FluoroNanogold on ultrathin cryosections. Proof of principle. Journal of Histochemistry and Cytochemistry 1998, 46:1097-1102.
    • (1998) Journal of Histochemistry and Cytochemistry , vol.46 , pp. 1097-1102
    • Takizawa, T.1    Suzuki, K.2    Robinson, J.M.3
  • 54
    • 33644645971 scopus 로고    scopus 로고
    • Correlative microscopy of ultrathin cryosections in placental research
    • Takizawa T., Robinson J.M. Correlative microscopy of ultrathin cryosections in placental research. Methods in Molecular Medicine 2006, 121:351-369.
    • (2006) Methods in Molecular Medicine , vol.121 , pp. 351-369
    • Takizawa, T.1    Robinson, J.M.2
  • 55
    • 0019042936 scopus 로고
    • Immunocytochemistry of ultrathin frozen sections
    • Tokuyasu K.T. Immunocytochemistry of ultrathin frozen sections. Histochemical Journal 1980, 12:381-403.
    • (1980) Histochemical Journal , vol.12 , pp. 381-403
    • Tokuyasu, K.T.1
  • 57
    • 56149101340 scopus 로고    scopus 로고
    • Correlative light-electron microscopy (CLEM) combining live-cell imaging and immunolabeling of ultrathin cryosections
    • van Rijnsoever C., Oorschot V.M., Klumperman J. Correlative light-electron microscopy (CLEM) combining live-cell imaging and immunolabeling of ultrathin cryosections. Nature Methods 2008, 5:973-980.
    • (2008) Nature Methods , vol.5 , pp. 973-980
    • van Rijnsoever, C.1    Oorschot, V.M.2    Klumperman, J.3
  • 59
    • 42649111676 scopus 로고    scopus 로고
    • Moving EM: The rapid transfer system as a new tool for correlative light and electron microscopy and high throughput for high-pressure freezing
    • Verkade P. Moving EM: The rapid transfer system as a new tool for correlative light and electron microscopy and high throughput for high-pressure freezing. Journal Microscopy 2008, 230:317-328.
    • (2008) Journal Microscopy , vol.230 , pp. 317-328
    • Verkade, P.1
  • 60
    • 53849089575 scopus 로고    scopus 로고
    • High data output and automated 3D correlative light-electron microscopy method
    • Vicidomini G., Gagliani M.C., Canfora M., Cortese K., Frosi F., Santangelo C., et al. High data output and automated 3D correlative light-electron microscopy method. Traffic 2008, 9:1828-1838.
    • (2008) Traffic , vol.9 , pp. 1828-1838
    • Vicidomini, G.1    Gagliani, M.C.2    Canfora, M.3    Cortese, K.4    Frosi, F.5    Santangelo, C.6


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