Definitive localization of intracellular proteins: Novel approach using CRISPR-Cas9 genome editing, with glucose 6-phosphate dehydrogenase as a model

Analytical Biochemistry

Volumn 494, Issue , 2016, Pages 55-67

  Spencer, Netanya Y ^a,b   Yan, Ziying ^c   Cong, Le ^d   Zhang, Yulong ^c   Engelhardt, John F ^c   Stanton, Robert C ^a,b,e  

^a JOSLIN DIABETES CENTER   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

^c UNIVERSITY OF IOWA   (United States)

^d BROAD INSTITUTE OF MIT AND HARVARD   (United States)

^e BETH ISRAEL DEACONESS MEDICAL CENTER   (United States)

Author keywords

CRISPR Cas9 genome engineering; Dimer; Glucose 6 phosphate dehydrogenase; Knockout cell lines; Multimer; Nuclear localization signal

Indexed keywords

CELL CULTURE; CELL ENGINEERING; DIMERIZATION; DIMERS; FLUORESCENCE; GENES; GLUCOSE; MOLECULAR BIOLOGY;

AMINO ACID SEQUENCE; CELL LINES; FLUORESCENT PROTEIN; GENOME ENGINEERING; INTRACELLULAR PROTEINS; MULTIMER; NUCLEAR LOCALIZATION SIGNAL; SUBCELLULAR LOCALIZATIONS;

PROTEINS;

CELL PROTEIN; CRISPR ASSOCIATED PROTEIN; CRISPR ASSOCIATED PROTEIN 9; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; MUTANT PROTEIN; UNCLASSIFIED DRUG; HYBRID PROTEIN; PHOTOPROTEIN; PRIMER DNA; RED FLUORESCENT PROTEIN;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; ARTIFACT; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CRISPR CAS SYSTEM; DIMERIZATION; FEMALE; GENE INACTIVATION; GENETIC ENGINEERING; GENETIC MODEL; GENETIC TRANSFECTION; GENOME; GOLD STANDARD; HELA CELL LINE; HUMAN; HUMAN CELL; LIVER CELL; MOLECULAR CLONING; MOUSE; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; PRIORITY JOURNAL; PROTEIN LOCALIZATION; SOMATIC CELL; ANIMAL; BIOSYNTHESIS; CELL LINE; EXON; FLUORESCENCE MICROSCOPY; GENETICS; METABOLISM;

ANIMALS; CELL LINE; CRISPR-CAS SYSTEMS; DIMERIZATION; DNA PRIMERS; EXONS; GENE KNOCKOUT TECHNIQUES; GENETIC ENGINEERING; GLUCOSEPHOSPHATE DEHYDROGENASE; HELA CELLS; HUMANS; LUMINESCENT PROTEINS; MICE; MICROSCOPY, FLUORESCENCE; RECOMBINANT FUSION PROTEINS;

EID: 84948664490     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2015.11.002     Document Type: Article

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