Nuclear localization of enhanced green fluorescent protein homomultimers

Analytical Biochemistry

Volumn 368, Issue 1, 2007, Pages 95-99

  Seibel, Nicole Maria ^a   Eljouni, Jihane ^a   Nalaskowski, Marcus Michael ^a   Hampe, Wolfgang ^a  

^a UNIVERSITY MEDICAL CENTER HAMBURG EPPENDORF   (Germany)

Author keywords

Dimer; EGFP; Hexamer; In gel fluorescence; Native electrophoresis; Subcellular localization; Tetramer; Trimer

Indexed keywords

CELL CULTURE; DIMERS; ELECTROPHORESIS; FLUORESCENCE; MAMMALS;

EGFP; HEXAMERS; SUBCELLULAR LOCALIZATIONS; TETRAMERS; TRIMER;

PROTEINS;

ENHANCED GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; POLYMER; TETRAMER;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; FLUORESCENCE; HUMAN; HUMAN CELL; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; NUCLEAR PORE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION;

MAMMALIA;

EID: 34447324079     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2007.05.025     Document Type: Article

References (27)

1. Tsien R.Y. The green fluorescent protein. Annu. Rev. Biochem. 67 (1998) 509-544
2. von Arnim A.G., Deng X.W., and Stacey M.G. Cloning vectors for the expression of green fluorescent protein fusion proteins in transgenic plants. Gene 221 (1998) 35-43
3. Grebenok R.J., Pierson E., Lambert G.M., Gong F.C., Afonso C.L., Haldeman-Cahill R., Carrington J.C., and Galbraith D.W. Green-fluorescent protein fusions for efficient characterization of nuclear targeting. Plant J. 11 (1997) 573-586
4. Bordonne R. Functional characterization of nuclear localization signals in yeast Sm proteins. Mol. Cell Biol. 20 (2000) 7943-7954
5. Minopoli G., de Candia P., Bonetti A., Faraonio R., Zambrano N., and Russo T. The β-amyloid precursor protein functions as a cytosolic anchoring site that prevents Fe65 nuclear translocation. J. Biol. Chem. 276 (2001) 6545-6550
6. Sheng Z., Lewis J.A., and Chirico W.J. Nuclear and nucleolar localization of 18-kDa fibroblast growth factor-2 is controlled by C-terminal signals. J. Biol. Chem. 279 (2004) 40153-40160
7. Borghi S., Molinari S., Razzini G., Parise F., Battini R., and Ferrari S. The nuclear localization domain of the MEF2 family of transcription factors shows member-specific features and mediates the nuclear import of histone deacetylase 4. J. Cell Sci. 114 (2001) 4477-4483
8. Bohm C., Seibel N., Henkel B., Steiner H., Haass C., and Hampe W. SorLA signaling by regulated intramembrane proteolysis. J. Biol. Chem. 281 (2006) 14547-14553
9. Macara I.G. Transport into and out of the nucleus. Microbiol. Mol. Biol. Rev. 65 (2001) 570-594
10. A. Heddad, M. Brameier, R. M. MacCallum, Evolving Regular Expression-based Sequence Classifiers for Protein Nuclear Localisation, www.sbc.su.se/∼maccallr/publications/heddad-evobio2004.pdf (2004).
11. Bhasin M., and Raghava G.P. ESLpred: SVM-based method for subcellular localization of eukaryotic proteins using dipeptide composition and PSI-BLAST. Nucleic Acids Res. 32 (2004) W414-W419
12. Cokol M., Nair R., and Rost B. Finding nuclear localization signals. EMBO Rep. 1 (2000) 411-415
13. Wei X., Henke V.G., Strubing C., Brown E.B., and Clapham D.E. Real-time imaging of nuclear permeation by EGFP in single intact cells. Biophys. J. 84 (2003) 1317-1327
14. Zheng C., Brownlie R., and Babiuk L.A. van Drunen Littel-van den Hurk, Characterization of the nuclear localization and nuclear export signals of bovine herpesvirus 1 VP22. J. Virol. 79 (2005) 11864-11872
15. Chatterjee S., Javier M., and Stochaj U. In vivo analysis of nuclear protein traffic in mammalian cells. Exp. Cell Res. 236 (1997) 346-350
16. Beetz C., Brodhun M., Moutzouris K., Kiehntopf M., Berndt A., Lehnert D., Deufel T., Bastmeyer M., and Schickel J. Identification of nuclear localisation sequences in spastin (SPG4) using a novel Tetra-GFP reporter system. Biochem. Biophys. Res. Commun. 318 (2004) 1079-1084
17. Faleiro L., and Lazebnik Y. Caspases disrupt the nuclear-cytoplasmic barrier. J. Cell Biol. 151 (2000) 951-959
18. Genove G., Glick B.S., and Barth A.L. Brighter reporter genes from multimerized fluorescent proteins. Biotechniques 39 (2005) 814-822
19. Wang W., and Malcolm B.A. Two-stage PCR protocol allowing introduction of multiple mutations, deletions and insertions using QuikChange Site-Directed Mutagenesis. Biotechniques 26 (1999) 680-682
20. 2+-permeable channel GRC. J. Cell Sci. 114 (2001) 3599-3606
21. Nalaskowski M.M., Windhorst S., Stockebrand M.C., and Mayr G.W. Subcellular localisation of human inositol 1,4,5-trisphosphate 3-kinase C: species-specific use of alternative export sites for nucleo-cytoplasmic shuttling indicates divergent roles of the catalytic and N-terminal domains. Biol. Chem. 387 (2006) 583-593
22. W.S. Rasband, ImageJ, U. S. National Institutes of Health, Bethesda, Maryland, USA. Available from: (1997-2006).
23. Abramoff M.D., Magelhaes P.J., and Ram S.J. Image processing with ImageJ. Biophotonics International 11 (2004) 36-42
24. Nalaskowski M.M., Deschermeier C., Fanick W., and Mayr G.W. The human homologue of yeast ArgRIII protein is an inositol phosphate multikinase with predominantly nuclear localization. Biochem. J. 366 (2002) 549-556
25. Park S.H., and Raines R.T. Green fluorescent protein as a signal for protein-protein interactions. Protein Sci. 6 (1997) 2344-2349
26. Kumar S., Saradhi M., Chaturvedi N.K., and Tyagi R.K. Intracellular localization and nucleocytoplasmic trafficking of steroid receptors: an overview. Mol. Cell Endocrinol. 246 (2006) 147-156
27. Clontech, Living Colors AcGFP1 Fluorescent Protein [brochure], www.clontech.com/images/brochures/AcGFP1.pdf.