RNA-guided genome editing of mammalian cells

Methods in Molecular Biology

Volumn 1114, Issue , 2014, Pages 269-277

  Pyzocha, Neena K ^a   Ran, F Ann ^b   Hsu, Patrick D ^a   Zhang, Feng ^a,b,c  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^c Massachusetts Institute of Technology Cambridge   (United States)

Author keywords

Cas9; CRISPR Cas; DNA cleavage; Gene knockout; Genome editing; Guide RNA; NHEJ; PAM sequence

Indexed keywords

CASPASE 9; DNA BINDING PROTEIN; GUIDE RNA; GENOMIC RNA;

ADAPTIVE IMMUNITY; ARTICLE; BASE PAIRING; CONTROLLED STUDY; CRISPR CAS SYSTEM; DNA CLEAVAGE; DNA END JOINING REPAIR; DNA MODIFICATION; DNA SEQUENCE; DOUBLE STRANDED DNA BREAK; GENE EXPRESSION; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; MAMMAL CELL; MOLECULAR CLONING; PRIORITY JOURNAL; PROMOTER REGION; RNA EDITING; STREPTOCOCCUS PYOGENES; CELL CULTURE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; EMBRYO; GENETIC TRANSFECTION; GENOME ANALYSIS; NONHUMAN;

ANIMALS; CELL LINE; CLONING, MOLECULAR; DNA END-JOINING REPAIR; GENE ORDER; GENE TARGETING; GENETIC VECTORS; GENOME; HUMANS; RNA EDITING; RNA, GUIDE; TRANSFECTION;

EUKARYOTA; MAMMALIA; STREPTOCOCCUS PYOGENES;

EID: 84922064723     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-761-7_17     Document Type: Article

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