Light and auxin responsive cytochrome P450s from Withania somnifera Dunal: cloning, expression and molecular modelling of two pairs of homologue genes with differential regulation

Protoplasma

Volumn 252, Issue 6, 2015, Pages 1421-1437

  Srivastava, Sudhakar ^a,c   Sangwan, Rajender Singh ^a,b   Tripathi, Sandhya ^a   Mishra, Bhawana ^a   Narnoliya, L K ^a   Misra, L N ^a   Sangwan, Neelam S ^a,b  

^a CSIR CENTRAL INSTITUTE OF MEDICINAL AND AROMATIC PLANTS   (India)

^b INSTITUTE OF NANO SCIENCE AND TECHNOLOGY   (India)

^c BEN GURION UNIVERSITY OF THE NEGEV   (Israel)

Author keywords

Ashwagandha; Cytochrome P450; Protein modelling; Withania somnifera; Withanolides

Indexed keywords

CYTOCHROME P450; INDOLEACETIC ACID DERIVATIVE; ISOENZYME; PLANT PROTEIN; RECOMBINANT PROTEIN; WITHANOLIDE;

BIOLOGY; BIOTRANSFORMATION; CHEMICAL STRUCTURE; CHEMISTRY; DNA SEQUENCE; DRUG EFFECTS; ENZYME SPECIFICITY; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETIC DATABASE; GENETICS; HYDROXYLATION; LIGHT; MEDICINAL PLANT; METABOLISM; MOLECULAR CLONING; PHYLOGENY; PROTEIN CONFORMATION; RADIATION RESPONSE; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; STRUCTURE ACTIVITY RELATION; WITHANIA;

BIOTRANSFORMATION; CLONING, MOLECULAR; COMPUTATIONAL BIOLOGY; CYTOCHROME P-450 ENZYME SYSTEM; DATABASES, GENETIC; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, PLANT; HYDROXYLATION; INDOLEACETIC ACIDS; ISOENZYMES; LIGHT; MODELS, MOLECULAR; PHYLOGENY; PLANT PROTEINS; PLANTS, MEDICINAL; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; SEQUENCE ANALYSIS, PROTEIN; SEQUENCE HOMOLOGY, AMINO ACID; SEQUENCE HOMOLOGY, NUCLEIC ACID; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY; WITHANIA; WITHANOLIDES;

EID: 84946487648     PISSN: 0033183X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00709-015-0766-9     Document Type: Article

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