Secretory expression of a phospholipase A2 from Lactobacillus casei DSM20011 in Kluyveromyces lactis

Journal of Bioscience and Bioengineering

Volumn 120, Issue 6, 2015, Pages 601-607

  Wang, Hui ^a   Zhang, Liang ^a   Shi, Guiyang ^a  

^a JIANGNAN UNIVERSITY   (China)

Author keywords

Copy number; Heterologous expression; Kluyveromyces lactis; Lactobacillus casei; Real time quantitative PCR; Secretory phospholipase A2

Indexed keywords

BOTTLES; CARBON; CLONING; GENES; POLYMERASE CHAIN REACTION; PROTEINS; RECOMBINANT PROTEINS;

COPY NUMBER; HETEROLOGOUS EXPRESSION; KLUYVEROMYCES LACTIS; LACTOBACILLUS CASEI; REAL-TIME QUANTITATIVE PCR; SECRETORY PHOSPHOLIPASE;

GENE ENCODING;

GALACTOSE; PHOSPHOLIPASE A2; RECOMBINANT PROTEIN; CULTURE MEDIUM; MATING FACTOR; PEPTIDE;

ARTICLE; CARBON SOURCE; CLONING; CULTURE MEDIUM; GENE; GENE DOSAGE; GENE LOCUS; GENE STRUCTURE; HETEROLOGOUS EXPRESSION; KLUYVEROMYCES LACTIS; LACTOBACILLUS CASEI; MOLECULAR WEIGHT; PH; PLA2 GENE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROMOTER REGION; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; BIOSYNTHESIS; CHEMISTRY; ENZYMOLOGY; GENE EXPRESSION; GENETICS; KLUYVEROMYCES; METABOLISM; SECRETION (PROCESS); TEMPERATURE;

CULTURE MEDIA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GALACTOSE; GENE DOSAGE; GENE EXPRESSION; HYDROGEN-ION CONCENTRATION; KLUYVEROMYCES; LACTOBACILLUS CASEI; MOLECULAR WEIGHT; PEPTIDES; PHOSPHOLIPASES A2; PROMOTER REGIONS, GENETIC; REAL-TIME POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; TEMPERATURE;

EID: 84946471274     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2015.03.022     Document Type: Article

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