Purification and characterization of the second Streptomyces phospholipase A2 refolded from an inclusion body

Protein Expression and Purification

Volumn 50, Issue 1, 2006, Pages 82-88

  Jovel, Santa Romero ^a   Kumagai, Takanori ^a   Danshiitsoodol, Narandalai ^a   Matoba, Yasuyuki ^a   Nishimura, Motohiro ^b   Sugiyama, Masanori ^a,c  

^a HIROSHIMA UNIVERSITY   (Japan)

^b UBE NATIONAL COLLEGE OF TECHNOLOGY   (Japan)

^c HIROSHIMA UNIVERSITY   (Japan)

Author keywords

Expression in Escherichia coli; Phospholipase A2; Streptomyces coelicolor

Indexed keywords

PHOSPHOLIPASE A;

AMINO ACID SEQUENCE; ARTICLE; CELL INCLUSION; CHEMICAL STRUCTURE; CHEMISTRY; CIRCULAR DICHROISM; ENZYME ACTIVATION; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; KINETICS; MOLECULAR CLONING; MOLECULAR GENETICS; PH; PROTEIN FOLDING; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT; STREPTOMYCES COELICOLOR; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; CIRCULAR DICHROISM; CLONING, MOLECULAR; CRYSTALLOGRAPHY, X-RAY; ENZYME ACTIVATION; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HYDROGEN-ION CONCENTRATION; INCLUSION BODIES; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PHOSPHOLIPASES A; PROTEIN FOLDING; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT; STREPTOMYCES COELICOLOR;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PROKARYOTA; STREPTOMYCES; STREPTOMYCES COELICOLOR; STREPTOMYCES VIOLACEORUBER;

EID: 33750486498     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.05.009     Document Type: Article

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