Functional expression and characterization of a recombinant phospholipase A2 from sea snake Lapemis hardwickii as a soluble protein in E. coli

Toxicon

Volumn 41, Issue 6, 2003, Pages 713-721

  Yang, Wen Li ^a   Peng, Li Sheng ^a   Zhong, Xiao Fen ^a   Wei, Jian Wen ^a   Jiang, Xiao Yu ^a   Ye, Lan Ting ^a   Zou, Lan ^a   Tu, Hong Bin ^a   Wu, Wen Yan ^a   Xu, An Long ^a  

^a SUN YAT SEN UNIVERSITY   (China)

Author keywords

Anti platelet aggregation effect; Phospholipase A2; Recombinant expression; Sea snake Lapemis hardwickii; Site directed mutagenesis

Indexed keywords

ASPARTIC ACID; COMPLEMENTARY DNA; ENTEROPEPTIDASE; HISTIDINE; HYBRID PROTEIN; METAL CHELATE; PHOSPHOLIPASE A2; PLASMID VECTOR; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; BACTERIUM CULTURE; ENZYMATIC ASSAY; ENZYMATIC DEGRADATION; ENZYME ACTIVITY; ENZYME MECHANISM; ESCHERICHIA COLI; EXPRESSION VECTOR; GENETIC CODE; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN INDUCTION; PROTEIN PROCESSING; PROTEIN PURIFICATION; RAT; SEA; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; SNAKE; SOLUBILITY; SPECIES COMPARISON; TEMPERATURE; THROMBOCYTE AGGREGATION;

AUSTRELAPS SUPERBUS; ESCHERICHIA COLI; HYDROPHIINAE; LAPEMIS HARDWICKII; SERPENTES;

EID: 0037406209     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0041-0101(03)00047-3     Document Type: Article

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