A novel recombinant system for functional expression of myonecrotic snake phospholipase A2 in Escherichia coli using a new fusion affinity tag

Protein Expression and Purification

Volumn 58, Issue 2, 2008, Pages 194-202

  Seto, Minae ^a   Ogawa, Tomohisa ^a   Kodama, Kyousuke ^a   Muramoto, Koji ^a   Kanayama, Yoshitaka ^b   Sakai, Yasuo ^b   Chijiwa, Takahito ^c   Ohno, Motonori ^c  

^a TOHOKU UNIVERSITY   (Japan)

^b Jellice Co Ltd   (Japan)

^c SOJO UNIVERSITY   (Japan)

Author keywords

Affinity tag; Chaperones; Congerin; Escherichia coli; Galectin; Microbacterium liquefaciens protease; Myotoxin; Phospholipase A2; Protobothrops flavoviridis; Recombinant expression; Snake venome; Thrombin

Indexed keywords

CONGERIN II PROTEIN, CONGER EEL; GALECTIN; HYBRID PROTEIN; PEPTIDE HYDROLASE; PHOSPHOLIPASE A2; SNAKE VENOM; THROMBIN; UNCLASSIFIED DRUG;

ACTINOMYCETALES; ARTICLE; BIOSYNTHESIS; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; ISOLATION AND PURIFICATION; METABOLISM;

ACTINOMYCETALES; CROTALID VENOMS; ESCHERICHIA COLI; GALECTINS; PEPTIDE HYDROLASES; PHOSPHOLIPASES A2; RECOMBINANT FUSION PROTEINS; THROMBIN;

CONGRIDAE; ESCHERICHIA COLI; MICROBACTERIUM LIQUEFACIENS; TRIMERESURUS FLAVOVIRIDIS;

EID: 39549083428     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2007.11.013     Document Type: Article

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