Expression of an active recombinant lysine 49 phospholipase A2 myotoxin as a fusion protein in bacteria

Toxicon

Volumn 39, Issue 10, 2001, Pages 1595-1600

  Giuliani, C D ^a   Iemma, M R C ^a   Bondioli, A C V ^a   Souza, D H F ^a   Ferreira, L L ^a   Amaral, A C ^a   Salvini, T F ^a   Selistre de Araujo, H S ^a  

^a FEDERAL UNIVERSITY OF SÃO CARLOS   (Brazil)

Author keywords

Expression; Myotoxin; Phospholipase A2; Refolding; Snake venom

Indexed keywords

HYBRID PROTEIN; LYSINE; MALTOSE BINDING PROTEIN; PHOSPHOLIPASE A2; TOXIN; TOXIN ANTIBODY;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BACTERIUM; BACTERIUM CULTURE; BACTERIUM TRANSFORMATION; CYTOPLASM; ESCHERICHIA COLI; EXPRESSION VECTOR; MOUSE; MUSCLE NECROSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; SKELETAL MUSCLE; STRUCTURE ANALYSIS;

AGKISTRODON; ANIMALS; ATP-BINDING CASSETTE TRANSPORTERS; BACTERIAL PROTEINS; CARRIER PROTEINS; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; CROTALID VENOMS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE LIBRARY; GENETIC VECTORS; INJECTIONS, INTRAMUSCULAR; ISOPROPYL THIOGALACTOSIDE; LYSINE; MALTOSE; MEMBRANE PROTEINS; MONOSACCHARIDE TRANSPORT PROTEINS; MUSCLE, SKELETAL; NECROSIS; NEUROTOXINS; PHOSPHOLIPASES A; PLASMIDS; POLYMERASE CHAIN REACTION; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; TOXINS, BIOLOGICAL; TRANSFORMATION, GENETIC;

EID: 0034894272     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0041-0101(01)00142-8     Document Type: Article

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