In vitro Th differentiation protocol

Methods in Molecular Biology

Volumn 1344, Issue , 2016, Pages 183-191

  Sekiya, Takashi ^a   Yoshimura, Akihiko ^a  

^a NONE

Author keywords

Cytokines; Helper T cell subsets; IL 12; IL 23; TGF

Indexed keywords

INTERLEUKIN 12; INTERLEUKIN 17; INTERLEUKIN 23; TRANSFORMING GROWTH FACTOR BETA; MEMBRANE ANTIGEN;

CELL CULTURE; CELL DIFFERENTIATION; CELL ISOLATION; CYTOKINE RELEASE; HUMAN; HUMAN CELL; IN VITRO STUDY; REGULATORY T LYMPHOCYTE; TH1 CELL; TH17 CELL; TH2 CELL; ANIMAL; CELL SEPARATION; CYTOLOGY; HELPER CELL; IMMUNOPHENOTYPING; METABOLISM; MOUSE; PHENOTYPE; PROCEDURES; T LYMPHOCYTE SUBPOPULATION;

ANIMALS; ANTIGENS, SURFACE; CELL DIFFERENTIATION; CELL SEPARATION; HUMANS; IMMUNOPHENOTYPING; IN VITRO TECHNIQUES; MICE; PHENOTYPE; T-LYMPHOCYTE SUBSETS; T-LYMPHOCYTES, HELPER-INDUCER;

EID: 84946430287     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2966-5_10     Document Type: Chapter

References (20)

1. Stritesky GL, Jameson SC, Hogquist KA (2012) Selection of self-reactive T cells in the thymus. Annu Rev Immunol 30:95–114, PMCID: 3518413
2. Sekiya T, Kashiwagi I, Inoue N, Morita R, Hori S, Waldmann H et al (2011) The nuclear orphan receptor Nr4a2 induces Foxp3 and regulates differentiation of CD4+ T cells. Nat Commun 2:269, PMCID: 3104557
3. Sekiya T, Kashiwagi I, Yoshida R, Fukaya T, Morita R, Kimura A et al (2013) Nr4a receptors are essential for thymic regulatory T cell development and immune homeostasis. Nat Immunol 14(3):230–237
4. Abbas AK, Murphy KM, Sher A (1996) Functional diversity of helper T lymphocytes. Nature 383(6603):787–793
5. Agnello D, Lankford CS, Bream J, Morinobu A, Gadina M, O’Shea JJ et al (2003) Cytokines and transcription factors that regulate T helper cell differentiation: new players and new insights. J Clin Immunol 23(3):147–161
6. Mowen KA, Glimcher LH (2004) Signaling pathways in Th2 development. Immunol Rev 202:203–222
7. Harrington LE, Hatton RD, Mangan PR, Turner H, Murphy TL, Murphy KM et al (2005) Interleukin 17-producing CD4+ effector T cells develop via a lineage distinct from the T helper type 1 and 2 lineages. Nat Immunol 6(11):1123–1132
8. Park H, Li Z, Yang XO, Chang SH, Nurieva R, Wang YH et al (2005) A distinct lineage of CD4 T cells regulates tissue inflammation by producing interleukin 17. Nat Immunol 6(11):1133–1141, PMCID: 1618871
9. Puel A, Cypowyj S, Bustamante J, Wright JF, Liu L, Lim HK et al (2011) Chronic mucocutaneous candidiasis in humans with inborn errors of interleukin-17 immunity. Science 332(6025):65–68, PMCID: 3070042
10. Zhu J, Yamane H, Paul WE (2010) Differentiation of effector CD4 T cell populations (*). Annu Rev Immunol 28:445–489, PMCID: 3502616
11. Hwang ES, Szabo SJ, Schwartzberg PL, Glimcher LH (2005) T helper cell fate specified by kinase-mediated interaction of T-bet with GATA-3. Science 307(5708):430–433
12. Veldhoen M, Hocking RJ, Atkins CJ, Locksley RM, Stockinger B (2006) TGFbeta in the context of an inflammatory cytokine milieu supports de novo differentiation of IL-17-producing T cells. Immunity 24(2):179–189
13. Mangan PR, Harrington LE, O’Quinn DB, Helms WS, Bullard DC, Elson CO et al (2006) Transforming growth factor-beta induces development of the T(H)17 lineage. Nature 441(7090):231–234
14. Ivanov II, McKenzie BS, Zhou L, Tadokoro CE, Lepelley A, Lafaille JJ et al (2006) The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17+ T helper cells. Cell 126(6):1121–1133
15. Bettelli E, Carrier Y, Gao W, Korn T, Strom TB, Oukka M et al (2006) Reciprocal developmental pathways for the generation of pathogenic effector TH17 and regulatory T cells. Nature 441(7090):235–238
16. Chung Y, Chang SH, Martinez GJ, Yang XO, Nurieva R, Kang HS et al (2009) Critical regulation of early Th17 cell differentiation by interleukin-1 signaling. Immunity 30(4):576–587, PMCID: 2705871
17. Cua DJ, Sherlock J, Chen Y, Murphy CA, Joyce B, Seymour B et al (2003) Interleukin-23 rather than interleukin-12 is the critical cytokine for autoimmune inflammation of the brain. Nature 421(6924):744–748
18. McGeachy MJ, Chen Y, Tato CM, Laurence A, Joyce-Shaikh B, Blumenschein WM et al (2009) The interleukin 23 receptor is essential for the terminal differentiation of interleukin 17-producing effector T helper cells in vivo. Nat Immunol 10(3):314–324, PMCID: 2945605
19. Takimoto T, Wakabayashi Y, Sekiya T, Inoue N, Morita R, Ichiyama K et al (2010) Smad2 and Smad3 are redundantly essential for the TGF-beta-mediated regulation of regulatory T plasticity and Th1 development. J Immunol 185(2):842–855
20. Lee Y, Awasthi A, Yosef N, Quintana FJ, Xiao S, Peters A et al (2012) Induction and molecular signature of pathogenic TH17 cells. Nat Immunol 13(10):991–999, PMCID: 3459594