Implementing bacterial acid resistance into cell-free protein synthesis for buffer-free expression and screening of enzymes

Biotechnology and Bioengineering

Volumn 112, Issue 12, 2015, Pages 2630-2635

  Kim, Ho Cheol ^a   Kim, Kwang Soo ^a   Kang, Taek Jin ^b   Choi, Jong Hyun ^c   Song, Jae Jun ^c   Choi, Yun Hee ^d   Kim, Byung Gee ^d   Kim, Dong Myung ^a  

^a Chungnam National University   (South Korea)

^b Dongguk University   (South Korea)

^c Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^d Seoul National University   (South Korea)

Author keywords

Cell free protein synthesis; Glutamate decarboxylase; High throughput expression and screening; PH homeostasis; 2,3 sialyltransferase

Indexed keywords

ACID RESISTANCE; AMINO ACIDS; CLONE CELLS; CYTOLOGY; ENZYMES; ESCHERICHIA COLI; GENE EXPRESSION; MACHINERY; MIXTURES; PH; THROUGHPUT;

BUFFERING CAPACITIES; CELL FREE PROTEIN SYNTHESIS; ENZYMATIC ACTIVITIES; GAMMA-AMINOBUTYRIC ACIDS; GLUTAMATE DECARBOXYLASE; HIGH THROUGHPUT; PH HOMEOSTASIS; RESISTANCE MECHANISMS;

BIOSYNTHESIS;

4 AMINOBUTYRIC ACID; ACID; ALPHA 2,3 SIALYLTRANSFERASE; BUFFER; GLUTAMATE DECARBOXYLASE; GLUTAMIC ACID; SIALYLTRANSFERASE; UNCLASSIFIED DRUG;

ARTICLE; CELL FREE SYSTEM; ENZYME ACTIVITY; ENZYME SYNTHESIS; ESCHERICHIA COLI; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; BIOSYNTHESIS; ENZYMOLOGY; GENETICS; MASS SCREENING; METABOLISM; PH; PROCEDURES; PROTEIN SYNTHESIS;

CELL-FREE SYSTEM; ESCHERICHIA COLI; GAMMA-AMINOBUTYRIC ACID; GLUTAMATE DECARBOXYLASE; GLUTAMIC ACID; HYDROGEN-ION CONCENTRATION; MASS SCREENING; PROTEIN BIOSYNTHESIS; SIALYLTRANSFERASES;

EID: 84945582675     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25671     Document Type: Article

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