Protein engineering of α2,3/2,6-sialyltransferase to improve the yield and productivity of in vitro sialyllactose synthesis

Glycobiology

Volumn 24, Issue 2, 2014, Pages 159-169

  Choi, Yun Hee ^a   Kim, Jong Hoon ^a   Park, Joon Ho ^b   Lee, Nahum ^b   Kim, Dae Hee ^c   Jang, Kyoung Soon ^a   Park, IL Hyang ^a   Kim, Byung Gee ^a,b,d  

^a Seoul National University   (South Korea)

^b Seoul National University   (South Korea)

^c Seoul National University   (South Korea)

^d GeneChem Inc   (South Korea)

Author keywords

hybrid approach; protein engineering; sialyllactose; sialyltransferase

Indexed keywords

ALANINE; ALPHA2,3 SIALYLTRANSFERASE; ALPHA2,6 SIALYLTRANSFERASE; SIALYLTRANSFERASE; UNCLASSIFIED DRUG;

ARTICLE; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME SUBSTRATE COMPLEX; ENZYME SYNTHESIS; IN VITRO STUDY; MUTATION; PH; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN FUNCTION; SEQUENCE ALIGNMENT; SITE DIRECTED MUTAGENESIS; WILD TYPE;

HYBRID APPROACH; PROTEIN ENGINEERING; SIALYLLACTOSE; SIALYLTRANSFERASE;

CLONING, MOLECULAR; LACTOSE; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; ORGANISMS, GENETICALLY MODIFIED; PASTEURELLA MULTOCIDA; PHOTOBACTERIUM; PROTEIN ENGINEERING; SEQUENCE ANALYSIS, DNA; SIALYLTRANSFERASES;

EID: 84893085090     PISSN: 09596658     EISSN: 14602423     Source Type: Journal    
DOI: 10.1093/glycob/cwt092     Document Type: Article

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