Overexpression and characterization of a glucose-tolerant β-glucosidase from T. aotearoense with high specific activity for cellobiose

Applied Microbiology and Biotechnology

Volumn 99, Issue 21, 2015, Pages 8903-8915

  Yang, Fang ^a   Yang, Xiaofeng ^a,c   Li, Zhe ^a   Du, Chenyu ^b   Wang, Jufang ^a   Li, Shuang ^a,d  

^a SOUTH CHINA UNIVERSITY OF TECHNOLOGY   (China)

^b UNIVERSITY OF HUDDERSFIELD   (United Kingdom)

^c CITY UNIVERSITY OF HONG KONG   (Hong Kong)

^d Guangzhou Higher Education Mega Center   (China)

Author keywords

Cellobiose degradation; Chaperones; Glucose tolerance; Thermoanaerobacterium aotearoense P8G3 4; Glucosidase

Indexed keywords

CLONING; ENCODING (SYMBOLS); ESCHERICHIA COLI; GENE ENCODING; GENE EXPRESSION; GLUCOSE; HYDROLASES; HYDROLYSIS; PH EFFECTS; PURIFICATION; SIGNAL ENCODING;

CELLOBIOSE; CHAPERONES; GLUCOSE TOLERANCE; GLUCOSIDASE; THERMOANAEROBACTERIUM;

ENZYME ACTIVITY;

BETA GLUCOSIDASE; BIOETHANOL; CELLOBIOSE; CHAPERONE; GLUCOSIDE HYDROLASE 1; GLYCOSIDASE; UNCLASSIFIED DRUG; ENZYME ACTIVATOR; ENZYME INHIBITOR; GLUCOSE; POTASSIUM; RECOMBINANT PROTEIN; SODIUM;

BACTERIUM; BIOTECHNOLOGY; CELLULOSE; CROP RESIDUE; DISACCHARIDE; ENZYME ACTIVITY; ETHANOL; GENE EXPRESSION; GLUCOSE; HYDROLYSIS; MOLECULAR ANALYSIS; PROTEIN; SUGAR CANE; THERMOREGULATION; TOLERANCE;

AMINO ACID SEQUENCE; ARTICLE; BIOFUEL PRODUCTION; CELLULAR DISTRIBUTION; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE OVEREXPRESSION; GENE SEQUENCE; GLUCONEOGENESIS; GLUCOSE TOLERANCE; INDUSTRIAL PRODUCTION; MICHAELIS MENTEN KINETICS; MICROBIAL BIOMASS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PH; REACTION TIME; THERMOANAEROBACTERIUM; THERMOANAEROBACTERIUM AOTEAROENSE; THERMOSTABILITY; CHEMICAL STRUCTURE; CHEMISTRY; DNA SEQUENCE; ENZYME STABILITY; ENZYMOLOGY; GENE EXPRESSION; GENETICS; GROWTH, DEVELOPMENT AND AGING; HYDROLYSIS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR GENETICS; PROTEIN CONFORMATION; SEQUENCE HOMOLOGY; TEMPERATURE;

ESCHERICHIA COLI; THERMOANAEROBACTERIUM AOTEAROENSE;

BETA-GLUCOSIDASE; CELLOBIOSE; CLONING, MOLECULAR; ENZYME ACTIVATORS; ENZYME INHIBITORS; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; GLUCOSE; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; OPEN READING FRAMES; POTASSIUM; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SODIUM; TEMPERATURE; THERMOANAEROBACTERIUM;

EID: 84945456621     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-015-6619-9     Document Type: Article

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