Robust orthogonal recombination system for versatile genomic elements rearrangement in yeast Saccharomyces Cerevisiae

Scientific Reports

Volumn 5, Issue , 2015, Pages

  Lin, Qiuhui ^a   Qi, Hao ^a   Wu, Yi ^a   Yuan, Yingjin ^a  

^a TIANJIN UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CRE RECOMBINASE; DNA POLYMERASE; INTEGRASE; SITE-SPECIFIC RECOMBINASE;

BUDDING YEAST; FUNGAL GENOME; GENE REARRANGEMENT; GENETIC RECOMBINATION; GENETICS; HOMOLOGOUS RECOMBINATION; METABOLISM; SACCHAROMYCES CEREVISIAE;

DNA NUCLEOTIDYLTRANSFERASES; GENE REARRANGEMENT; GENOME, FUNGAL; HOMOLOGOUS RECOMBINATION; INTEGRASES; RECOMBINATION, GENETIC; SACCHAROMYCES CEREVISIAE; SACCHAROMYCETALES;

EID: 84945237302     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep15249     Document Type: Article

References (25)

1. Carr, P. A., Church, G. M. Genome engineering. Nature biotechnology 27, 1151-1162 (2009).
2. Shao, Z., Zhao, H. Construction and engineering of large biochemical pathways via DNA assembler. Methods Mol Biol 1073, 85-106 (2013).
3. Gibson, D. G. et al. Creation of a bacterial cell controlled by a chemically synthesized genome. Science 329, 52-56 (2010).
4. Henry, C., Overbeek, R., Stevens, R. L. Building the blueprint of life. Biotechnology journal 5, 695-704 (2010).
5. Isaacs, F. J. et al. Precise manipulation of chromosomes in vivo enables genome-wide codon replacement. Science 333, 348-353 (2011).
6. Giovannoni, S. J. et al. Genome streamlining in a cosmopolitan oceanic bacterium. Science 309, 1242-1245 (2005).
7. Swan, B. K. et al. Prevalent genome streamlining and latitudinal divergence of planktonic bacteria in the surface ocean. Proceedings of the National Academy of Sciences of the United States of America 110, 11463-11468 (2013).
8. Shao, Z., Zhao, H. DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways. Nucleic acids research 37, e16 (2009).
9. Gibson, D. G. et al. Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome. Science 319, 1215-1220 (2008).
10. Brown, W. R., Lee, N. C., Xu, Z., Smith, M. C. Serine recombinases as tools for genome engineering. Methods 53, 372-379 (2011).
11. Grindley, N. D., Whiteson, K. L., Rice, P. A. Mechanisms of site-specific recombination. Annual review of biochemistry 75, 567-605 (2006).
12. Dymond, J. S. et al. Synthetic chromosome arms function in yeast and generate phenotypic diversity by design. Nature 477, 471-476 (2011).
13. Annaluru, N. et al. Total synthesis of a functional designer eukaryotic chromosome. Science 344, 55-58 (2014).
14. Saraf-Levy, T. et al. Site-specific recombination of asymmetric lox sites mediated by a heterotetrameric Cre recombinase complex. Bioorganic & medicinal chemistry 14, 3081-3089 (2006).
15. Lauth, M., Spreafico, F., Dethleffsen, K., Meyer, M. Stable and efficient cassette exchange under non?selectable conditions by combined use of two site?specific recombinases. Nucleic acids research 30, e115-e115 (2002).
16. Guo, F., Gopaul, D. N., van Duyne, G. D. Structure of Cre recombinase complexed with DNA in a site-specific recombination synapse. Nature 389, 40-46 (1997).
17. Dymond, J., Boeke, J. The Saccharomyces cerevisiae SCRaMbLE system and genome minimization. Bioengineered bugs 3, 168-171 (2012).
18. Dujon, B. Yeast evolutionary genomics. Nature reviews. Genetics 11, 512-524 (2010).
19. Krivoruchko, A., Siewers, V., Nielsen, J. Opportunities for yeast metabolic engineering: Lessons from synthetic biology. Biotechnology journal 6, 262-276 (2011).
20. Stephanopoulos, G. Synthetic biology and metabolic engineering. ACS synthetic biology 1, 514-525 (2012).
21. Cheng, A. A., Lu, T. K. Synthetic biology: an emerging engineering discipline. Annual review of biomedical engineering 14, 155-178 (2012).
22. Minorikawa, S., Nakayama, M. Recombinase-mediated cassette exchange (RMCE) and BAC engineering via VCre/VloxP and SCre/SloxP systems. BioTechniques 50, 235-246 (2011).
23. Suzuki, E., Nakayama, M. VCre/VloxP and SCre/SloxP: new site-specific recombination systems for genome engineering. Nucleic acids research 39, e49 (2011).
24. Karimova, M. et al. Vika/vox, a novel efficient and specific Cre/loxP-like site-specific recombination system. Nucleic acids research 41, e37 (2013).
25. Hoess, R. H., Wierzbicki, A., Abremski, K. The role of the loxP spacer region in P1 site-specific recombination. Nucleic acids research 14, 2287-2300 (1986).