Directed Evolution of a Panel of Orthogonal T7 RNA Polymerase Variants for in Vivo or in Vitro Synthetic Circuitry

ACS Synthetic Biology

Volumn 4, Issue 10, 2015, Pages 1070-1076

  Meyer, Adam J ^a   Ellefson, Jared W ^a   Ellington, Andrew D ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

directed evolution; genetic circuits; transcription

Indexed keywords

ENZYME VARIANT; RNA POLYMERASE; T7 RNA POLYMERASE; UNCLASSIFIED DRUG; BACTERIOPHAGE T7 RNA POLYMERASE; DNA DIRECTED RNA POLYMERASE; VIRUS PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; ENZYME SPECIFICITY; ESCHERICHIA COLI; EVOLUTION; IN VITRO STUDY; IN VIVO STUDY; MUTANT; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; WILD TYPE; GENETICS;

DNA-DIRECTED RNA POLYMERASES; PROMOTER REGIONS, GENETIC; VIRAL PROTEINS;

EID: 84944720278     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/sb500299c     Document Type: Article

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