Coupled cytoplasmic transcription-and-translation - A method of choice for heterologous gene expression in Xenopus oocytes

Journal of Biotechnology

Volumn 122, Issue 1, 2006, Pages 5-15

  Tokmakov, Alexander A ^a   Matsumoto, Eiko ^a   Shirouzu, Mikako ^a   Yokoyama, Shigeyuki ^a,b,c  

^a RIKEN YOKOHAMA INSTITUTE   (Japan)

^b RIKEN HARIMA INSTITUTE   (Japan)

^c UNIVERSITY OF TOKYO   (Japan)

Author keywords

Expression technique; Luciferase; T7 RNA polymerase; Xenopus oocytes

Indexed keywords

BIODIVERSITY; CELLS; DNA; ENZYMES; GENES; RNA;

EXPRESSION TECHNIQUE; LUCIFERASE; T7 RNA POLYMERASE; XENOPUS OOCYTES;

GENETIC ENGINEERING;

LUCIFERASE; NUCLEIC ACID; PLASMID DNA; RNA POLYMERASE;

ANIMAL TISSUE; ARTICLE; CELL FUNCTION; CELL VIABILITY; CONTROLLED STUDY; CYTOPLASM; DNA TEMPLATE; FEMALE; GENETIC TRANSCRIPTION; HETEROLOGOUS EXPRESSION; NONHUMAN; OOCYTE; PRIORITY JOURNAL; PROTEIN SYNTHESIS; REPRODUCIBILITY; RNA TRANSLATION; XENOPUS;

ANIMALS; BACTERIOPHAGE T7; CYTOPLASM; DNA-DIRECTED RNA POLYMERASES; GENETIC VECTORS; OOCYTES; PROTEIN BIOSYNTHESIS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; TRANS-ACTIVATION (GENETICS); TRANSFECTION; VIRAL PROTEINS; XENOPUS LAEVIS;

ANIMALIA;

EID: 32644464668     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2005.08.027     Document Type: Article

References (25)

1. W.A. Alexander, B. Moss, and T.R. Fuerst Regulated expression of foreign genes in vaccinia virus under the control of bacteriophage T7 RNA polymerase and the Escherichia coli lac repressor J. Virol. 66 1992 2934 2942
2. C.C. Allende, J.E. Allende, and R.A. Firtel The degradation of ribonucleic acids injected into Xenopus laevis oocytes Cell 2 1974 189 196
3. M.M. Bendig, and J.G. Williams Fidelity of transcription of Xenopus laevis globin genes injected into Xenopus laevis oocytes and unfertilized eggs Mol. Cell. Biol. 4 1984 2109 2119
4. X. Chen, Y. Li, K. Xiong, and T.E. Wagner A self-initiating eukaryotic transient gene expression system based on cotransfection of bacteriophage T7 RNA polymerase and DNA vectors containing aT7 autogene Nucleic Acids Res. 22 1994 2114 2120
5. P. Davanloo, A.H. Rosenberg, J.J. Dunn, and F.W. Studier Cloning and expression of the gene for bacteriophage T7 RNA polymerase Proc. Natl. Acad. Sci. U.S.A. 81 1984 2035 2039
6. H. Deng, C. Wang, G. Acsadi, and J.A. Wolff High efficiency protein synthesis from T7 RNA polymerase transcripts in 3T3 fibroblasts Gene 109 1991 193 201
7. J.J. Dunn, B. Krippl, K.E. Bernstein, H. Westphal, and F.W. Studier Targeting bacteriophage T7 polymerase to the mammalian cell nucleus Gene 68 1988 259 266
8. O. Elroy-Stein, T.R. Fuerst, and B. Moss Cap-independent translation of mRNA conferred by encephalomyocarditis virus 5′ sequence improves the performance of the vaccinia virus/bacteriophage T7 hybrid expression system Proc. Natl. Acad. Sci. U.S.A. 86 1989 6126 6130
9. O. Elroy-Stein, and B. Moss Cytosolic expression system based on constitutive synthesis of bacteriophage T7 RNA polymerase in mammalian cells Proc. Natl. Acad. Sci. U.S.A. 87 1990 6743 6747
10. X. Gao, and L. Huang Cytoplasmic expression of a reporter gene by co-delivery of T7 RNA polymerase and T7 promoter sequence with cationic liposomes Nucleic Acids Res. 21 1993 2867 2872
11. S. Geib, G. Sandoz, E. Carlier, V. Cornet, V. Cheynet-Sauvion, and M. De Waard A novel Xenopus oocyte expression system based on cytoplasmic coinjection of T7-driven plasmids and purified T7-RNA polymerase Receptors Channels 7 2001 331 343
12. A.C. Gingras, B. Raught, and N. Sonenberg eIF4 initiation factors: effector of mRNA recruitment to ribosome and regulators of translation Ann. Rev. Biochem. 68 1999 913 963
13. J.B. Gurdon, and D.A. Melton Gene transfer in amphibian eggs and oocytes Ann. Rev. Genet. 15 1981 189 218
14. B. He, M. Rong, D. Lyakhov, H. Gartenstein, G. Diaz, R. Castagna, W.T. McAllister, and R.K. Durbin Rapid mutagenesis and purification of phage RNA polymerases Protein Exp. Purif. 9 1997 142 151
15. Z. Jin, D. Lu, H. Huang, H. Wang, W. Pei, and L. Luo Expression of firefly luciferase gene in Xenopus laevis oocyte Chin. J. Biotechnol. 7 1991 279 284
16. J.F. Klement, M.B. Moorefield, E. Jorgensen, J.E. Brown, S. Risman, and W.T. McAllister Discrimination between bacteriophage T3 and T7 promoters by the T3 and T7 RNA polymerases depends primarily upon a three base-pair region located 10-12 base-pairs upstream from the start site J. Mol. Biol. 215 1990 21 29
17. D. Luo, and W.M. Saltzman Synthetic DNA delivery systems Nat. Biotechnol. 18 2000 33 37
18. L.B. Lutz, B. Kim, D. Yahani, and S.R. Hammes G protein βγ subunits inhibit nongenomic progesterone-induced signaling and maturation in Xenopus laevis oocytes J. Biol. Chem. 275 2000 41512 41520
19. S.L. Pfaff, M.M. Tamkun, and W.L. Taylor pOEV: a Xenopus oocyte protein expression vector Anal. Biochem. 188 1990 192 199
20. R.G. Roeder Multiple forms of deoxyribonucleic acid-dependent ribonucleic acid polymerase in Xenopus laevis. Levels of activity during oocyte and embryonic development J. Biol. Chem. 249 1974 249 256
21. J. Sambrook, and D.W. Russell Separation of RNA according to size: electrophoresis of RNA through agarose gels containing formaldehyde Molecular Cloning: A Laboratory Manual third ed. 2001 Cold Spring Harbor Laboratory Press Cold Spring Harbor, NY pp. 7.31-7.34
22. K. Shiokawa, C. Koga, Y. Ito, and M. Shibata Expression of exogenous genes in Xenopus oocytes, eggs, and embryos R. Tuan Methods in Molecular Biology 1997 Humana Press Inc. Totowa, NJ 247 270
23. A.A. Tokmakov, K.-I. Sato, T. Iwasaki, and Y. Fukami Src kinase induces calcium release in Xenopus egg extract via PLCγ and IP3-dependent mechanism Cell Calcium 32 2002 11 20
24. A.H. Wyllie, R.A. Laskey, J. Flinch, and J.B. Gurdon Selective DNA conservation and chromatin assembly after injection of SV40 DNA into Xenopus oocytes Dev. Biol. 64 1978 178 188
25. D.L. Yim, L.K. Opresko, H.S. Wiley, and R. Nuccitelli Highly polarized EGF receptor tyrosine kinase activity initiates egg activation in Xenopus Dev. Biol. 162 1994 41 55