New insights into the QuikChangeTM process guide the use of Phusion DNA polymerase for site-directed mutagenesis

Nucleic Acids Research

Volumn 43, Issue 2, 2015, Pages e12-

  Xia, Yongzhen ^a   Chu, Wenqiao ^a   Qi, Qingsheng ^a   Xun, Luying ^a,b  

^a SHANDONG UNIVERSITY   (China)

^b WASHINGTON STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; DNA DIRECTED DNA POLYMERASE; PRIMER DNA;

BIOSYNTHESIS; CHEMISTRY; ESCHERICHIA COLI; GENETIC RECOMBINATION; GENETICS; PLASMID; POLYMERASE CHAIN REACTION; PROCEDURES; SITE DIRECTED MUTAGENESIS;

DNA; DNA PRIMERS; DNA-DIRECTED DNA POLYMERASE; ESCHERICHIA COLI; MUTAGENESIS, SITE-DIRECTED; PLASMIDS; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC;

EID: 84944678735     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gku1189     Document Type: Article

References (21)

1. Marini,F., Naeem,A. and Lapeyre,J. N. (1993) An efficient 1-tube PCR method for internal site-directed mutagenesis of large amplified molecules. Nucleic Acids Res., 21, 2277-2278.
2. Picard,V., Ersdal-Badju,E., Lu,A. and Bock,S. C. (1994) A rapid and efficient one-tube PCR-based mutagenesis technique using Pfu DNA polymerase. Nucleic Acids Res., 22, 2587-2591.
3. Brøns-Poulsen,J., Petersen,N., Hørder,M. and Kristiansen,K. (1998) An improved PCR-based method for site directed mutagenesis using megaprimers. Mol. Cell. Probes, 12, 345-348.
4. Zheng,L., Baumann,U. and Reymond,J. L. (2004) An efficient one-step site-directed and site-saturation mutagenesis protocol. Nucleic Acids Res., 32, e115.
5. Ho,S. N., Hunt,H. D., Horton,R. M., Pullen,J. K. and Pease,L. R. (1989) Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene, 77, 51-59.
6. Liu,H. and Naismith,J. H. (2008) An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol. BMC Biotechnol., 8, 91.
7. Jones,D. H., Sakamoto,K., Vorce,R. L. and Howard,B. H. (1990) DNA mutagenesis and recombination. Nature, 344, 793-794.
8. Qi,D. and Scholthof,K. B. (2008) A one-step PCR-based method for rapid and efficient site-directed fragment deletion, insertion, and substitution mutagenesis. J. Virol. Methods, 149, 85-90.
9. Wu,D., Guo,X., Lu,J., Sun,X., Li,F., Chen,Y. and Xiao,D. (2013) A rapid and efficient one-step site-directed deletion, insertion, and substitution mutagenesis protocol. Anal. Biochem., 434, 254-258.
10. Li,J., Li,C., Xiao,W., Yuan,D.,Wan,G. and Ma,L. (2008) Site-directed mutagenesis by combination of homologous recombination and DpnI digestion of the plasmid template in Escherichia coli. Anal. Biochem., 373, 389-391.
11. Chung,C. T., Niemela,S. L. and Miller,R. H. (1989) One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proc. Natl Acad. Sci. U. S. A., 86, 2172-2175.
12. Fu,J., Bian,X., Hu,S., Wang,H., Huang,F., Seibert,P. M., Plaza,A., Xia,L., Muller,R., Stewart,A. F. et al. (2012) Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting. Nat. Biotechnol., 30, 440-446.
13. Langley,K. E., Villarejo,M. R., Fowler,A. V., Zamenhof,P. J. and Zabin,I. (1975) Molecular basis of beta-galactosidase alpha-complementation. Proc. Natl Acad. Sci. U. S. A., 72, 1254-1257.
14. Kovach,M. E., Elzer,P. H., Hill,D. S., Robertson,G. T., Farris,M. A., Roop,R. M. and Peterson,K. M. (1995) Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene, 166, 175-176.
15. Gibson,D. G., Young,L., Chuang,R. Y., Venter,J. C., Hutchison,C. A. and Smith,H. O. (2009) Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat. Methods, 6, 343-345.
16. Owczarzy,R., Tataurov,A. V.,Wu,Y., Manthey,J. A., McQuisten,K. A., Almabrazi,H. G., Pedersen,K. F., Lin,Y., Garretson,J., McEntaggart,N. O. et al. (2008) IDT SciTools: a suite for analysis and design of nucleic acid oligomers. Nucleic Acids Res., 36, W163-W169.
17. Al-Soud,W. A. and Radstrom,P. (2001) Purification and characterization of PCR-inhibitory components in blood cells. J. Clin. Microbiol., 39, 485-493.
18. Datsenko,K. A. and Wanner,B. L. (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl Acad. Sci. U. S. A., 97, 6640-6645.
19. Jiang,W., Bikard,D., Cox,D., Zhang,F. and Marraffini,L. A. (2013) RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat. Biotechnol., 31, 233-239.
20. Qi,L. S., Larson,M. H., Gilbert,L. A., Doudna,J. A., Weissman,J. S., Arkin,A. P. and Lim,W. A. (2013) Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell, 152, 1173-1183.
21. Parrish,J. R., Limjindaporn,T., Hines,J. A., Liu,J., Liu,G. and Finley,R. L. Jr (2004) High-throughput cloning of Campylobacter jejuni ORfs by in vivo recombination in Escherichia coli. J. Proteome Res., 3, 582-586.