Purification and characterization of PCR-inhibitory components in blood cells

Journal of Clinical Microbiology

Volumn 39, Issue 2, 2001, Pages 485-493

  Al Soud, W A ^a   Radstrom P ^a  

^a LUND UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE; HEMOGLOBIN; IMMUNOGLOBULIN G; LACTOFERRIN; POLYPEPTIDE; SERUM ALBUMIN; SINGLE STRANDED DNA BINDING PROTEIN;

AMINO TERMINAL SEQUENCE; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; BLOOD CELL; BLOOD COMPONENT; DEVICE; DNA SYNTHESIS; DNA TEMPLATE; ERYTHROCYTE; GEL PERMEATION CHROMATOGRAPHY; HUMAN; HUMAN CELL; LEUKOCYTE; LISTERIA MONOCYTOGENES; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN PURIFICATION; SEQUENCE ANALYSIS; THERMOSTABILITY;

BLOOD PLATELETS; BLOOD PROTEINS; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; DNA, BACTERIAL; DNA-DIRECTED DNA POLYMERASE; ERYTHROCYTES; HEMOGLOBINS; HUMANS; LACTOFERRIN; LEUKOCYTES; LISTERIA MONOCYTOGENES; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; TEMPLATES, GENETIC;

BOVINAE; LISTERIA MONOCYTOGENES;

EID: 0035126283     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.39.2.485-493.2001     Document Type: Article

References (56)

1. Identification and characterization of immunoglobulin G in blood as a major inhibitor of diagnostic PCR
2. A sample preparation method which facilitates detection of bacteria in blood cultures by the polymerase chain reaction
3. Capacity of nine thermostable DNA polymerases to mediate DNA amplification in the presence of PCRinhibiting samples
4. Identification of the heme compound copurified with deoxyribonucleic acid (DNA) from bloodstains, a major inhibitor of polymerase chain reaction (PCR) amplification
5. Purification of highly degraded DNA by gel filtration for PCR
6. Structure of human lactoferrin: Crystallographic structure analysis and refinement at 2.8 A resolution
7. Differential inhibition of multiple forms of DNA polymerase alpha from IMR-32 human neuroblastoma cells
8. Polymerase chain reaction (PCR) detection of Listeria monocytogenes in diluted milk and reversal of PCR inhibition caused by calcium ions
9. Mechanism of hemin inhibition of erythroid cytoplasmic DNA polymerase
10. Comparison of three DNA extraction methods on bone and blood stains up to 43 years old and amplification of three different gene sequences
11. Reverse transcriptase (RT) inhibition of PCR at low concentrations of template and its implications for quantitative RT-PCR
12. Single-stranded DNA binding proteins required for DNA replication
13. Crystal structure of human serum albumin complexed with fatty acid
14. The use of polymerase chain reaction to detect septicemia in critically ill patients
15. A new role for lactoferrin: DNA binding and transcription activation
16. Betaine and DMSO: Enhancing agents for PCR
17. Effect of heat denaturation of target DNA on the PCR amplification
18. Sequence specificity and transcriptional activation in the binding of lactoferrin to DNA
19. Simple and rapid preparation of samples for PCR
20. Lactoferrin markedly inhibits hepatitis C virus infection in cultured human hepatocytes
21. Mechanism of inhibition of mammalian DNA topoisomerase 1 by heparin
22. Reversal of RT-PCR inhibition observed in heparinized clinical specimens
23. A distinctive property of Tth DNA polymerase: Enzymatic amplification in the presence of phenol
24. Rapid analysis and purification of polymerase chain reaction products by high-performance liquid chromatography
25. Comparison of methods for extraction of nucleic acid from hemolytic serum for PCR amplification of hepatitis B virus DNA sequences
26. Relief of amplification inhibition in PCR with bovine serum albumin or T4 gene 32 protein
27. Bactericidal domain of lactoferrin: Detection, quantitation, and characterization of lactoferricin in serum by SELDI affinity mass spectrometry
28. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
29. Removal of PCR inhibitors from human faecal samples through the use of an aqueous two-phase system for sample preparation prior to PCR
30. Enhanced sensitivity in PCR detection of Listeria monocytogenes in soft cheese through use of an aqueous two-phase system as a sample preparation method
31. Lactoferrin: Molecular structure and biological function
32. DNA enzyme immunoassay: General method for detecting products of polymerase chain reaction
33. Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis
34. Gene localization for an autosomal dominant familial periodic fever to 12p13
35. PCR based diagnosis in the presence of 8% (v/v) blood
36. In vitro proteolysis of human plasma low density lipoproteins by an elastase released from human blood polymorphonuclear cells
37. Proteinase inhibition of the detection of Listeria monocytogenes in milk using the polymerase chain reaction
38. Detection of bacterial DNA in cerebrospinal fluid by an assay for simultaneous detection of Neisseria meningitidis. Haemophilus influenzae, and streptococci using a seminested PCR strategy
39. Evaluation of the AmpliSensor PCR and the SHARP signal detection system for the early prediction of symptomatic CMV infection in solid transplant recipients
40. Product differentiation by analysis of DNA melting curves during the polymerase chain reaction
41. International standardization of gene amplification technology
42. Inhibition of PCR by components of food samples, microbial diagnostic assays and DNA-extraction solution
43. Genetic analysis of amplified DNA with immobilized sequence-specific oligonucleotide probes
44. Increased thermal stability of proteins in the presence of naturally occurring osmolytes
45. Effect of heparin on polymerase chain reaction
46. Products of bacteriophage T4 genes 32 and 45 improve the accuracy of DNA replication in vitro
47. Heroin inhibits virion-associated reverse transcriptase of murine leukemia virus
48. Chelex ® 100 as medium for simple extraction of DNA for PCR-based typing from forensic material
49. Effects of anticoagulants and storage of blood samples on efficacy of the polymerase chain reaction assay for hepatitis C virus
50. The magnetic immuno polymerase chain reaction assay for direct detection of salmonellae in fecal samples
51. Inhibition of PCR by aqueous and vitreous fluids
52. Primary structure of the bacteriophage T4 DNA helix-destabilizing protein
53. Continuous fluorescence monitoring of rapid cycle DNA amplification
54. Detection of Streptococcus pneumoniae in whole blood by PCR