Highly efficient CRISPR/HDR-mediated knock-in for mouse embryonic stem cells and zygotes

BioTechniques

Volumn 59, Issue 4, 2015, Pages 201-208

  Wang, Bangmei ^a   Li, Kunyu ^a   Wang, Amy ^a   Reiser, Michelle ^a   Saunders, Thom ^b   Lockey, Richard F ^a,c   Wang, Jia Wang ^a  

^a UNIVERSITY OF SOUTH FLORIDA   (United States)

^b UNIVERSITY OF MICHIGAN   (United States)

^c VETERANS AFFAIRS MEDICAL CENTER   (United States)

Author keywords

CRISPR cleavage; Double strand break (DSB); Embryonic stem (ES) cells; Homology directed repair (HDR); Zygote

Indexed keywords

DNA FRAGMENT; DNA;

ANIMAL CELL; APOPTOSIS; ARTICLE; BLASTOCYST; CELL VIABILITY; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONTROLLED STUDY; DNA END JOINING REPAIR; DNA SEQUENCE; DOUBLE STRANDED DNA BREAK; ELECTROPORATION; EMBRYO; EMBRYONIC STEM CELL; GENE FUNCTION; GENE SILENCING; GENE TARGETING; HOMOLOGOUS RECOMBINATION; MOUSE; NONHUMAN; POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING; SURVIVAL RATE; ZYGOTE; ANIMAL; CRISPR CAS SYSTEM; GENETIC ENGINEERING; GENETICS; METABOLISM; MOUSE EMBRYONIC STEM CELL; RECOMBINATION REPAIR; RNA EDITING;

ANIMALS; CRISPR-CAS SYSTEMS; DNA; DNA END-JOINING REPAIR; GENE KNOCK-IN TECHNIQUES; GENETIC ENGINEERING; MICE; MOUSE EMBRYONIC STEM CELLS; RECOMBINATIONAL DNA REPAIR; RNA EDITING; ZYGOTE;

EID: 84944058188     PISSN: 07366205     EISSN: 19409818     Source Type: Journal    
DOI: 10.2144/000114339     Document Type: Article

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