Artifact-Free Quantification and Sequencing of Rare Recombinant Viruses by Using Drop-Based Microfluidics

ChemBioChem

Volumn 16, Issue 15, 2015, Pages 2167-2171

  Tao, Ye ^a,b   Rotem, Assaf ^a   Zhang, Huidan ^a,c   Cockrell, Shelley K ^d   Koehler, Stephan A ^a   Chang, Connie B ^a,e   Ung, Lloyd W ^a   Cantalupo, Paul G ^d   Ren, Yukun ^b   Lin, Jeffrey S ^f   Feldman, Andrew B ^f,g   Wobus, Christiane E ^h   Pipas, James M ^d   Weitz, David A ^a  

^a HARVARD UNIVERSITY   (United States)

^b HARBIN INSTITUTE OF TECHNOLOGY   (China)

^c CHINA MEDICAL UNIVERSITY   (China)

^d UNIVERSITY OF PITTSBURGH   (United States)

^e Montana State University   (United States)

^f JOHNS HOPKINS UNIVERSITY APPLIED PHYSICS LABORATORY   (United States)

^g JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^h UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

drop based microfluidics; error free genomic amplification; RT PCR; sequence determination; viruses

Indexed keywords

VIRUS RNA; FLUORESCENT DYE;

ANIMAL CELL; ARTICLE; ARTIFACT; DROP BASED MICROFLUIDICS; GENE AMPLIFICATION; GENE SEQUENCE; GENETIC RECOMBINATION; HIGH THROUGHPUT SEQUENCING; IN VITRO STUDY; MACROPHAGE CULTURE; MICROFLUIDIC ANALYSIS; MICROFLUIDICS; NONHUMAN; PRIORITY JOURNAL; PROGENY; PROGRAM COST EFFECTIVENESS; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRAL GENETICS; VIRUS RECOMBINANT; VIRUS RECOMBINATION; ANIMAL; CELL CULTURE; GENETICS; HIGH THROUGHPUT SCREENING; MOUSE; PARTICLE SIZE; PROCEDURES; SEQUENCE ANALYSIS; VIRUS; VIRUS REPLICATION;

ANIMALS; ARTIFACTS; CELLS, CULTURED; FLUORESCENT DYES; HIGH-THROUGHPUT SCREENING ASSAYS; MICE; MICROFLUIDICS; PARTICLE SIZE; RECOMBINATION, GENETIC; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; VIRUS REPLICATION; VIRUSES;

EID: 84943357780     PISSN: 14394227     EISSN: 14397633     Source Type: Journal    
DOI: 10.1002/cbic.201500384     Document Type: Article

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