Analysis of 454 sequencing error rate, error sources, and artifact recombination for detection of Low-frequency drug resistance mutations in HIV-1 DNA

Retrovirology

Volumn 10, Issue 1, 2013, Pages

  Shao, Wei ^a   Boltz, Valerie F ^b   Spindler, Jonathan E ^b   Kearney, Mary F ^b   Maldarelli, Frank ^b   Mellors, John W ^c   Stewart, Claudia ^a   Volfovsky, Natalia ^a   Levitsky, Alexander ^a   Stephens, Robert M ^a   Coffin, John M ^b,d  

^a SAIC FREDERICK INC   (United States)

^b NATIONAL CANCER INSTITUTE   (United States)

^c UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^d TUFTS UNIVERSITY   (United States)

Author keywords

454 pyrosequencing; Error rate; HIV 1; PCR induced recombination

Indexed keywords

DNA POLYMERASE; VIRUS DNA;

ANTIVIRAL RESISTANCE; ARTICLE; CONTROLLED STUDY; DNA TEMPLATE; ERROR; GENE AMPLIFICATION; GENE FREQUENCY; GENETIC RECOMBINATION; HAPLOTYPE; HUMAN IMMUNODEFICIENCY VIRUS 1; INDEL MUTATION; LINKAGE ANALYSIS; MOLECULAR CLONING; MUTATION RATE; NONHUMAN; NUCLEIC ACID BASE SUBSTITUTION; POINT MUTATION; POLYMERASE CHAIN REACTION; PYROSEQUENCING; SEQUENCE ANALYSIS; SEQUENCING ERROR; VIRUS MUTATION; WILD TYPE;

ARTIFACTS; DRUG RESISTANCE, VIRAL; HIV INFECTIONS; HIV-1; HUMANS; MICROBIAL SENSITIVITY TESTS; MOLECULAR SEQUENCE DATA; MUTATION; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC; RESEARCH DESIGN; SEQUENCE ANALYSIS, DNA;

EID: 84875002547     PISSN: None     EISSN: 17424690     Source Type: Journal    
DOI: 10.1186/1742-4690-10-18     Document Type: Article

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