Biochemical isolation of Argonaute protein complexes by Ago-APP

Proceedings of the National Academy of Sciences of the United States of America

Volumn 112, Issue 38, 2015, Pages 11841-11845

  Hauptmann, Judith ^a   Schraivogel, Daniel ^a   Bruckmann, Astrid ^a   Manickavel, Sudhir ^b   Jakob, Leonhard ^a   Eichner, Norbert ^a   Pfaff, Janina ^a   Urban, Marc ^a   Sprunck, Stefanie ^a   Hafner, Markus ^b   Tuschl, Thomas ^c   Deutzmann, Rainer ^a   Meister, Gunter ^a   Hannon, Gregory J ^d  

^a UNIVERSITY OF REGENSBURG   (Germany)

^b NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES   (United States)

^c ROCKEFELLER UNIVERSITY   (United States)

^d Simons Center for Quantitative Biology   (United States)

Author keywords

Argonaute; GW proteins; MicroRNAs; RNAi; Small RNAs

Indexed keywords

ARGONAUTE PROTEIN; GLUTATHIONE TRANSFERASE; MESSENGER RNA; MICRORNA; PEPTIDE; TNRC6 PEPTIDE; UNCLASSIFIED DRUG; CELL EXTRACT; MULTIPROTEIN COMPLEX;

ANALYTIC METHOD; ANIMAL CELL; ARGONAUTE PROTEIN AFFINITY PURIFICATION BY PEPTIDES; ARTICLE; BINDING AFFINITY; CELL LYSATE; COMPLEX FORMATION; CONTROLLED STUDY; HUMAN; HUMAN CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN ISOLATION; PROTEIN PURIFICATION; AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ANIMAL; CHEMISTRY; DROSOPHILA MELANOGASTER; GENE SILENCING; HEK293 CELL LINE; HELA CELL LINE; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; PRECIPITATION; PROCEDURES;

AMINO ACID SEQUENCE; ANIMALS; ARGONAUTE PROTEINS; CELL EXTRACTS; CHEMICAL PRECIPITATION; CHROMATOGRAPHY, AFFINITY; DROSOPHILA MELANOGASTER; GENE SILENCING; HEK293 CELLS; HELA CELLS; HUMANS; MICRORNAS; MOLECULAR SEQUENCE DATA; MULTIPROTEIN COMPLEXES; PEPTIDES;

EID: 84942893749     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1506116112     Document Type: Article

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