Turning catalytically inactive human Argonaute proteins into active slicer enzymes

Nature Structural and Molecular Biology

Volumn 20, Issue 7, 2013, Pages 814-817

  Hauptmann, Judith ^a   Dueck, Anne ^a   Harlander, Simone ^a   Pfaff, Janina ^a   Merkl, Rainer ^a   Meister, Gunter ^a  

^a UNIVERSITY OF REGENSBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; ARGONAUTE 1 PROTEIN; ARGONAUTE 3 PROTEIN; ARGONAUTE PROTEIN; COMPLEMENTARY RNA; ENDONUCLEASE; ENZYME; HEMAGGLUTININ; MICRORNA; MUTANT PROTEIN; PIWI PROTEIN; RIBONUCLEASE T1; RNA INDUCED SILENCING COMPLEX; SMALL INTERFERING RNA; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CATALYSIS; CELL STRAIN HEK293; CONTROLLED STUDY; ENZYME ACTIVATION; GENE MUTATION; GENE SILENCING; HUMAN; HUMAN CELL; IN VITRO STUDY; MUTAGENESIS; NORTHERN BLOTTING; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; PROTEIN STRUCTURE; RNA CLEAVAGE; TRANSPOSON; WESTERN BLOTTING;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARGONAUTE PROTEINS; BASE SEQUENCE; CATALYSIS; EUKARYOTIC INITIATION FACTORS; HEK293 CELLS; HUMANS; MICRORNAS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; POINT MUTATION; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; RNA INTERFERENCE; RNA PROCESSING, POST-TRANSCRIPTIONAL; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 84880168667     PISSN: 15459993     EISSN: 15459985     Source Type: Journal    
DOI: 10.1038/nsmb.2577     Document Type: Article

References (27)

1. Ender, C. & Meister, G. Argonaute proteins at a glance. J. Cell Sci. 123, 1819-1823 (2010).
2. Hutvagner, G. & Simard, M.J. Argonaute proteins: key players in RNA silencing. Nat. Rev. Mol. Cell Biol. 9, 22-32 (2008).
3. Jinek, M. & Doudna, J.A. A three-dimensional view of the molecular machinery of RNA interference. Nature 457, 405-412 (2009).
4. Tolia, N.H. & Joshua-Tor, L. Slicer and the argonautes. Nat. Chem. Biol. 3, 36-43 (2007).
5. Siomi, M.C., Sato, K., Pezic, D. & Aravin, A.A. PIWI-interacting small RNAs: the vanguard of genome defence. Nat. Rev. Mol. Cell Biol. 12, 246-258 (2011).
6. Nakanishi, K., Weinberg, D.E., Bartel, D.P. & Patel, D.J. Structure of yeast Argonaute with guide RNA. Nature 486, 368-374 (2012).
7. Liu, J. et al. Argonaute2 is the catalytic engine of mammalian RNAi. Science 305, 1437-1441 (2004).
8. Meister, G. et al. Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs. Mol. Cell 15, 185-197 (2004).
9. Yekta, S., Shih, I.H. & Bartel, D.P. MicroRNA-directed cleavage of HOXB8 mRNA. Science 304 594-596 (2004).
10. Cheloufi, S., Dos Santos, C.O., Chong, M.M. & Hannon, G.J. A dicer-independent miRNA biogenesis pathway that requires Ago catalysis. Nature 465, 584-589 (2010).
11. Cifuentes, D. et al. A novel miRNA processing pathway independent of Dicer requires Argonaute2 catalytic activity. Science 328, 1694-1698 (2010).
12. Yang, J.S. et al. Conserved vertebrate mir-451 provides a platform for Dicer-independent, Ago2-mediated microRNA biogenesis. Proc. Natl. Acad. Sci. USA 107, 15163-15168 (2010).
13. Kim, V.N., Han, J. & Siomi, M.C. Biogenesis of small RNAs in animals. Nat. Rev. Mol. Cell Biol. 10, 126-139 (2009).
14. Matranga, C., Tomari, Y., Shin, C., Bartel, D.P. & Zamore, P.D. Passenger-strand cleavage facilitates assembly of siRNA into Ago2-containing RNAi enzyme complexes. Cell 123, 607-620 (2005).
15. Rand, T.A., Petersen, S., Du, F. & Wang, X. Argonaute2 cleaves the anti-guide strand of siRNA during RISC activation. Cell 123, 621-629 (2005).
16. Leuschner, P.J., Ameres, S.L., Kueng, S. & Martinez, J. Cleavage of the siRNA passenger strand during RISC assembly in human cells. EMBO Rep. 7 314-320 (2006).
17. Petri, S. et al. Increased siRNA duplex stability correlates with reduced off-target and elevated on-target effects. RNA 17, 737-749 (2011).
18. Gu, S. et al. Thermodynamic stability of small hairpin RNAs highly influences the loading process of different mammalian Argonautes. Proc. Natl. Acad. Sci. USA 108, 9208-9213 (2011).
19. Wang, B. et al. Distinct passenger strand and mRNA cleavage activities of human Argonaute proteins. Nat. Struct. Mol. Biol. 16, 1259-1266 (2009).
20. Schirle, N.T. & MacRae, I.J. The crystal structure of human Argonaute2. Science 336, 1037-1040 (2012).
21. Elkayam, E. et al. The structure of human argonaute-2 in complex with miR-20a. Cell 150, 100-110 (2012).
22. Hur, J.K., Zinchenko, M.K., Djuranovic, S. & Green, R. Regulation of Argonaute slicer activity by guide RNA 3 end interactions with the N-terminal lobe. J. Biol. Chem. 288, 7829-7840 (2013).
23. Kwak, P.B. & Tomari, Y. The N domain of Argonaute drives duplex unwinding during RISC assembly. Nat. Struct. Mol. Biol. 19, 145-151 (2012).
24. Meister, G. et al. Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs. Mol. Cell 15, 185-197 (2004).
25. Pall, G.S. & Hamilton, A.J. Improved northern blot method for enhanced detection of small RNA. Nat. Protoc. 3, 1077-1084 (2008).
26. Roy, A., Kucukural, A. & Zhang, Y. I-TASSER: a unified platform for automated protein structure and function prediction. Nat. Protoc. 5, 725-738 (2010).
27. Kabsch, W. & Sander, C. Dictionary of protein secondary structure: pattern recognition of hydrogen-bonded and geometrical features. Biopolymers 22, 2577-2637 (1983).