Combined cell-surface display- and secretion-based strategies for production of cellulosic ethanol with Saccharomyces cerevisiae

Biotechnology for Biofuels

Volumn 8, Issue 1, 2015, Pages

  Liu, Zhuo ^a   Inokuma, Kentaro ^a   Ho, Shih Hsin ^a,b   Haan, Riaan Den ^c   Hasunuma, Tomohisa ^a   Van Zyl, Willem H ^d   Kondo, Akihiko ^a,e  

^a KOBE UNIVERSITY   (Japan)

^b HARBIN INSTITUTE OF TECHNOLOGY   (China)

^c UNIVERSITY OF THE WESTERN CAPE   (South Africa)

^d STELLENBOSCH UNIVERSITY   (South Africa)

^e RIKEN   (Japan)

Author keywords

Cell surface display; Cellulase; Ethanol; Saccharomyces cerevisiae; Secretion

Indexed keywords

CELL MEMBRANES; CELLS; CELLULOSE; CELLULOSIC ETHANOL; CYTOLOGY; ENZYMES; ETHANOL; FERMENTATION; PHYSIOLOGY;

AMORPHOUS CELLULOSE; CELL SURFACE DISPLAYS; CELLOBIOHYDROLASE I; CELLULASE; CELLULOSE DEGRADATION; ETHANOL FERMENTATION; PHOSPHORIC-ACID SWOLLEN CELLULOSE; SECRETION;

YEAST;

BIOFUEL; CELLULOSE; ENZYME; ETHANOL; FERMENTATION; SECRETION;

CELLULOSE; ENZYMES; ETHANOL; FERMENTATION; PHOSPHORIC ACID; YEASTS;

SACCHAROMYCES CEREVISIAE;

EID: 84942422812     PISSN: 17546834     EISSN: None     Source Type: Journal    
DOI: 10.1186/s13068-015-0344-6     Document Type: Article

References (50)

1. Nigam PS, Singh A. Production of liquid biofuels from renewable resources. Prog Energy Combust Sci. 2011;37:52-68.
2. Limayem A, Ricke SC. Lignocellulosic biomass for bioethanol production: current perspectives, potential issues and future prospects. Prog Energy Combust Sci. 2012;38:449-67.
3. Garvey M, Klose H, Fischer R, Lambertz C, Commandeur U. Cellulases for biomass degradation: comparing recombinant cellulase expression platforms. Trends Biotechnol. 2013;31:581-93.
4. Matano Y, Hasunuma T, Kondo A. Display of cellulases on the cell surface of Saccharomyces cerevisiae for high yield ethanol production from high-solid lignocellulosic biomass. Bioresour Technol. 2012;108:128-33.
5. Yamada R, Taniguchi N, Tanaka T, Ogino C, Fukuda H, Kondo A. Direct ethanol production from cellulosic materials using a diploid strain of Saccharomyces cerevisiae with optimized cellulase expression. Biotechnol Biofuels. 2011;4:8.
6. den Haan R, Rose SH, Lynd LR, van Zyl WH. Hydrolysis and fermentation of amorphous cellulose by recombinant Saccharomyces cerevisiae. Metab Eng. 2007;9:87-94.
7. Kondo A, Ueda M. Yeast cell-surface display-applications of molecular display. Appl Microbiol Biotechnol. 2004;64:28-40.
8. Bae J, Kuroda K, Ueda M. Proximity effect among cellulose-degrading enzymes displayed on the Saccharomyces cerevisiae cell surface. Appl Environ Microbiol. 2015;81:59-66.
9. Hasunuma T, Kondo A. Consolidated bioprocessing and simultaneous saccharification and fermentation of lignocellulose to ethanol with thermotolerant yeast strains. Process Biochem. 2012;47:1287-94.
10. Sanda T, Hasunuma T, Matsuda F, Kondo A. Repeated-batch fermentation of lignocellulosic hydrolysate to ethanol using a hybrid Saccharomyces cerevisiae strain metabolically engineered for tolerance to acetic and formic acids. Bioresour Technol. 2011;102:7917-24.
11. Kondo A, Shigechi H, Abe M, Uyama K, Matsumoto T, Takahashi S, Ueda M, Tanaka A, Kishimoto M, Fukuda H. High-level ethanol production from starch by a flocculent Saccharomyces cerevisiae strain displaying cell-surface glucoamylase. Appl Microbiol Biotechnol. 2002;58:291-6.
12. den Haan R, van Rensburg E, Rose SH, Görgens JF, van Zyl WH. Progress and challenges in the engineering of non-cellulolytic microorganisms for consolidated bioprocessing. Curr Opin Biotechnol. 2015;33:32-8.
13. Resch MG, Donohoe BS, Baker JO, Decker SR, Bayer EA, Beckham GT, Himmel ME. Fungal cellulases and complexed cellulosomal enzymes exhibit synergistic mechanisms in cellulose deconstruction. Energy Environ Sci. 2013;6:1858-67.
14. Arantes V, Saddler JN. Access to cellulose limits the efficiency of enzymatic hydrolysis: the role of amorphogenesis. Biotechnol Biofuels. 2010;3:4.
15. Pack SP, Park K, Yoo YJ. Enhancement of β-glucosidase stability and cellobiose-usage using surface-engineered recombinant Saccharomyces cerevisiae in ethanol production. Biotechnol Lett. 2002;24:1919-25.
16. van Zyl WH, Lynd LR, den Haan R, McBride JE. Consolidated bioprocessing for bioethanol production using Saccharomyces cerevisiae. Adv Biochem Eng/Biotechnol. 2007;108:205-35.
17. Hu J, Gourlay K, Arantes V, Van Dyk JS, Pribowo A, Saddler JN. The accessible cellulose surface influences cellulase synergism during the hydrolysis of lignocellulosic substrates. ChemSusChem. 2015;8:901-7.
18. den Haan R, Mcbride JE, Grange DCL, Lynd LR, Van Zyl WH. Functional expression of cellobiohydrolases in Saccharomyces cerevisiae towards one-step conversion of cellulose to ethanol. Enzyme Microb Tech. 2007;40:1291-9.
19. Takada G, Kawaguchi T, Sumitani J, Arai M. Expression of Aspergillus aculeatus No. F-50 cellobiohydrolase I (cbhI) and beta-glucosidase 1 (bgl1) genes by Saccharomyces cerevisiae. Biosci Biotechnol Biochem. 1998;62:1615-8.
20. den Haan R, Kroukamp H, van Zyl JH-D, van Zyl WH. Cellobiohydrolase secretion by yeast: current state and prospects for improvement. Process Biochem. 2013;48:1-12.
21. Ilmén M, Den Haan R, Brevnova E, McBride J, Wiswall E, Froehlich A, Koivula A, Voutilainen SP, Siika-Aho M, la Grange DC. High level secretion of cellobiohydrolases by Saccharomyces cerevisiae. Biotechnol Biofuels. 2011;4:30.
22. Inokuma K, Hasunuma T, Kondo A. Efficient yeast cell-surface display of exo-and endo-cellulase using the SED1 anchoring region and its original promoter. Biotechnol Biofuels. 2014;7:8.
23. Fujita Y, Ito J, Ueda M, Fukuda H, Kondo A. Synergistic saccharification, and direct fermentation to ethanol, of amorphous cellulose by use of an engineered yeast strain codisplaying three types of cellulolytic enzyme. Appl Environ Microbiol. 2004;70:1207-12.
24. Andersen N, Johansen KS, Michelsen M, Stenby EH, Krogh KBRM, Olsson L. Hydrolysis of cellulose using mono-component enzymes shows synergy during hydrolysis of phosphoric acid swollen cellulose (PASC), but competition on Avicel. Enzyme Microb Tech. 2008;42:362-70.
25. Bunterngsook B, Eurwilaichitr L, Thamchaipenet A, Champreda V. Binding characteristics and synergistic effects of bacterial expansins on cellulosic and hemicellulosic substrates. Bioresour Technol. 2015;176:129-35.
26. Reyes-Ortiz V, Heins RA, Cheng G, Kim EY, Vernon BC, Elandt RB, Adams PD, Sale KL, Hadi MZ, Simmons BA, et al. Addition of a carbohydrate-binding module enhances cellulase penetration into cellulose substrates. Biotechnol Biofuels. 2013;6:93.
27. Shimoi H, Kitagaki H, Ohmori H, Iimura Y, Ito K. Sed1p is a major cell wall protein of Saccharomyces cerevisiae in the stationary phase and is involved in lytic enzyme resistance. J Bacteriol. 1998;180:3381-7.
28. Yanase S, Yamada R, Kaneko S, Noda H, Hasunuma T, Tanaka T, Ogino C, Fukuda H, Kondo A. Ethanol production from cellulosic materials using cellulase-expressing yeast. Biotechnol J. 2010;5:449-55.
29. Griggs AJ, Stickel JJ, Lischeske JJ. A mechanistic model for enzymatic saccharification of cellulose using continuous distribution kinetics I: depolymerization by EGI and CBHI. Biotechnol Bioeng. 2012;109:665-75.
30. Peckys DB, Mazur P, Gould KL, de Jonge N. Fully hydrated yeast cells imaged with electron microscopy. Biophys J. 2011;100:2522-9.
31. Ju X, Grego C, Zhang X. Specific effects of fiber size and fiber swelling on biomass substrate surface area and enzymatic digestibility. Bioresour Technol. 2013;144:232-9.
32. Lynd LR, Weimer PJ, Van Zyl WH, Pretorius IS. Microbial cellulose utilization: fundamentals and biotechnology. Microbiol Mol Biol Rev. 2002;66:506-77.
33. Cruys-Bagger N, Elmerdahl J, Praestgaard E, Tatsumi H, Spodsberg N, Borch K, Westh P. Pre-steady-state kinetics for hydrolysis of insoluble cellulose by cellobiohydrolase Cel7A. J Biol Chem. 2012;287:18451-8.
34. Kurasin M, Valjamae P. Processivity of cellobiohydrolases is limited by the substrate. J Biol Chem. 2011;286:169-77.
35. Francisco JA, Stathopoulos C, Warren RA, Kilburn DG, Georgiou G. Specific adhesion and hydrolysis of cellulose by intact Escherichia coli expressing surface anchored cellulase or cellulose binding domains. Bio/Technology. 1993;11:491-5.
36. Fan C, Qi K, Xia X-X, Zhong J-J. Efficient ethanol production from corncob residues by repeated fermentation of an adapted yeast. Bioresour Technol. 2013;136:309-15.
37. Jin M, Gunawan C, Uppugundla N, Balan V, Dale BE. A novel integrated biological process for cellulosic ethanol production featuring high ethanol productivity, enzyme recycling and yeast cells reuse. Energy Environ Sci. 2012;5:7168-75.
38. Watanabe I, Miyata N, Ando A, Shiroma R, Tokuyasu K, Nakamura T. Ethanol production by repeated-batch simultaneous saccharification and fermentation (SSF) of alkali-treated rice straw using immobilized Saccharomyces cerevisiae cells. Bioresour Technol. 2012;123:695-8.
39. Khaw TS, Katakura Y, Koh J, Kondo A, Ueda M, Shioya S. Evaluation of performance of different surface-engineered yeast strains for direct ethanol production from raw starch. Appl Microbiol Biotechnol. 2006;70:573-9.
40. Matano Y, Hasunuma T, Kondo A. Cell recycle batch fermentation of high-solid lignocellulose using a recombinant cellulase-displaying yeast strain for high yield ethanol production in consolidated bioprocessing. Bioresour Technol. 2013;135:403-9.
41. Brevnova E, McBride J, Wiswall E, Wenger K, Caiazza N, Hau H, Argyros A, Agbogbo F, Rice C, Barret T, Bardsley J, Foster A, Warner A, Mellon M, Skinner R, Shikhare I, Den Haan R, Gandhi C, Bekcher A, Rajgarhia V, Froehlich A, Deleault K, Stonehouse E, Tripathi S, Gosselin J, Chiu YY, Xu H Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose. PCT/US2011/039192(WO/2011/153516). 12-8-2011. 3-6-0110.
42. McBride JE, Brevnova E, Ghandi C, Mellon M, Froehlich A, Delaault K, Rajgarhia V, Flatt J, Van Zyl WH, Den Haan R, La Grange DC, Rose SH, Penttilä M, Ilmen M, Siika-aho M, Uusitalo J, Hau HH, Rice C, Villari J, Stonehouse EA, Gilbert A, Keating JD, Xu H, Willes D, Shikhare I, Thorngren N, Warner AK, Murphy D Yeast expressing cellulases for simultaneous saccharification and fermentation using cellulose. PCT/US2009/065571. 5-27-2010.
43. Gibson DG, Young L, Chuang R-Y, Venter JC, Hutchison CA, Smith HO. Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat Methods. 2009;6:343-5.
44. Chen D-C, Yang B-C, Kuo T-T. One-step transformation of yeast in stationary phase. Curr Genet. 1992;21:83-4.
45. Ismail KSK, Sakamoto T, Hatanaka H, Hasunuma T, Kondo A. Gene expression cross-profiling in genetically modified industrial Saccharomyces cerevisiae strains during high-temperature ethanol production from xylose. J Biotechnol. 2013;163:50-60.
46. Yamada R, Taniguchi N, Tanaka T, Ogino C, Fukuda H, Kondo A. Cocktail δ-integration: a novel method to construct cellulolytic enzyme expression ratio-optimized yeast strains. Microb Cell Fact. 2010;9:32.
47. Yamada R, Nakatani Y, Ogino C, Kondo A. Efficient direct ethanol production from cellulose by cellulase-and cellodextrin transporter-co-expressing Saccharomyces cerevisiae. AMB Express. 2013;3:34.
48. Kalapos MP. Methylglyoxal in living organisms: chemistry, biochemistry, toxicology and biological implications. Toxicol Lett. 1999;110:145-75.
49. Yamada R, Yoshie T, Sakai S, Wakai S, Asai-Nakashima N, Okazaki F, Ogino C, Hisada H, Tsutsumi H, Hata Y. Effective saccharification of kraft pulp by using a cellulase cocktail prepared from genetically engineered Aspergillus oryzae. Biosci Biotechnol Biochem. 2015. doi: 10.1080/09168451.2015.1006568.
50. Inokuma K, Yoshida T, Ishii J, Hasunuma T, Kondo A. Efficient co-displaying and artificial ratio control of α-amylase and glucoamylase on the yeast cell surface by using combinations of different anchoring domains. Appl Microbiol Biotechnol. 2014;99:1655-63.