High-level production of membrane proteins in E. coli BL21(DE3) by omitting the inducer IPTG

Microbial Cell Factories

Volumn 14, Issue 1, 2015, Pages

  Zhang, Zhe ^a   Kuipers, Grietje ^b   Niemiec, Łukasz ^a   Baumgarten, Thomas ^a   Slotboom, Dirk Jan ^c   de Gier, Jan Willem ^a   Hjelm, Anna ^a  

^a STOCKHOLM UNIVERSITY   (Sweden)

^b Xbrane Bioscience AB ^*  (Sweden)

^c UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

BL21(DE3); Escherichia coli; Membrane protein; Protein production; Secretory protein; T7 RNA polymerase

Indexed keywords

ISOPROPYL THIOGALACTOSIDE; MEMBRANE PROTEIN; SECRETORY PROTEIN; GREEN FLUORESCENT PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; BACTERIAL CELL; BACTERIAL STRAIN; CELL CULTURE; CELL MEMBRANE; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; CYTOPLASM; ESCHERICHIA COLI; NONHUMAN; PROTEIN AGGREGATION; PROTEIN ANALYSIS; PROTEIN SYNTHESIS; BIOREACTOR; BIOSYNTHESIS; CELL CULTURE TECHNIQUE; GENE VECTOR; GENETICS; METABOLIC ENGINEERING; METABOLISM; PROCEDURES;

BIOREACTORS; CELL CULTURE TECHNIQUES; ESCHERICHIA COLI; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; ISOPROPYL THIOGALACTOSIDE; MEMBRANE PROTEINS; METABOLIC ENGINEERING; RECOMBINANT PROTEINS;

EID: 84941645959     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-015-0328-z     Document Type: Article

References (29)

1. Makino T, Skretas G, Georgiou G. Strain engineering for improved expression of recombinant proteins in bacteria. Microb Cell Fact. 2011;10:32.
2. Schlegel S, Klepsch M, Gialama D, Wickstrom D, Slotboom DJ, de Gier JW. Revolutionizing membrane protein overexpression in bacteria. Microb Biotechnol. 2010;3(4):403-11.
3. Studier FW. Stable expression clones and auto-induction for protein production in E. coli. Methods Mol Biol. 2014;1091:17-32.
4. Studier FW, Moffatt BA. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol. 1986;189(1):113-30.
5. Chamberlin M, McGrath J, Waskell L. New RNA polymerase from Escherichia coli infected with bacteriophage T7. Nature. 1970;228(5268):227-31.
6. Iost I, Guillerez J, Dreyfus M. Bacteriophage T7 RNA polymerase travels far ahead of ribosomes in vivo. J Bacteriol. 1992;174(2):619-22.
7. Arditti RR, Scaife JG, Beckwith JR. The nature of mutants in the lac promoter region. J Mol Biol. 1968;38(3):421-6.
8. Wanner BL, Kodaira R, Neidhardt FC. Physiological regulation of a decontrolled lac operon. J Bacteriol. 1977;130(1):212-22.
9. Silverstone AE, Arditti RR, Magasanik B. Catabolite-insensitive revertants of lac promoter mutants. Proc Natl Acad Sci USA. 1970;66(3):773-9.
10. Schlegel S, Hjelm A, Baumgarten T, Vikstrom D, de Gier JW. Bacterial-based membrane protein production. Biochim Biophys Acta. 2014;1843(8):1739-49.
11. Luirink J, Yu Z, Wagner S, de Gier JW. Biogenesis of inner membrane proteins in Escherichia coli. Biochim Biophys Acta. 2012;1817(6):965-76.
12. Wagner S, Baars L, Ytterberg AJ, Klussmeier A, Wagner CS, Nord O, Nygren PA, van Wijk KJ, de Gier JW. Consequences of membrane protein overexpression in Escherichia coli. Mol Cell Proteomics. 2007;6(9):1527-50.
13. Wagner S, Klepsch MM, Schlegel S, Appel A, Draheim R, Tarry M, Hogbom M, van Wijk KJ, Slotboom DJ, Persson JO, et al. Tuning Escherichia coli for membrane protein overexpression. Proc Natl Acad Sci USA. 2008;105(38):14371-6.
14. Drew D, Fröderberg L, Baars L, de Gier JW. Assembly and overexpression of membrane proteins in Escherichia coli. Biochim Biophys Acta. 2003;1610(1):3-10.
15. Studier FW. Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system. J Mol Biol. 1991;219(1):37-44.
16. Miroux B, Walker JE. Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels. J Mol Biol. 1996;260:289-98.
17. Alfasi S, Sevastsyanovich Y, Zaffaroni L, Griffiths L, Hall R, Cole J. Use of GFP fusions for the isolation of Escherichia coli strains for improved production of different target recombinant proteins. J Biotechnol. 2011;156(1):11-21.
18. Schlegel S, Genevaux P, de Gier JW. De-convoluting the genetic adaptations of E. coli C41(DE3) in real time reveals how alleviating protein production stress improves yields. Cell Rep. 2015;10(10):1758-66.
19. Grossman TH, Kawasaki ES, Punreddy SR, Osburne MS. Spontaneous cAMP-dependent derepression of gene expression in stationary phase plays a role in recombinant expression instability. Gene. 1998;209(1-2):95-103.
20. Nair R, Salvi P, Banerjee S, Raiker VA, Bandyopadhyay S, Soorapaneni S, Kotwal P, Padmanabhan S. Yeast extract mediated autoinduction of lacUV5 promoter: an insight. N Biotechnol. 2009;26(6):282-8.
21. Schlegel S, Lofblom J, Lee C, Hjelm A, Klepsch M, Strous M, Drew D, Slotboom DJ, de Gier JW. Optimizing Membrane Protein Overexpression in the Escherichia coli strain Lemo21(DE3). J Mol Biol. 2012;423(4):648-59.
22. Drew D, Lerch M, Kunji E, Slotboom DJ, de Gier JW. Optimization of membrane protein overexpression and purification using GFP fusions. Nat Methods. 2006;3(4):303-13.
23. Geertsma ER, Groeneveld M, Slotboom DJ, Poolman B. Quality control of overexpressed membrane proteins. Proc Natl Acad Sci USA. 2008;105(15):5722-7.
24. Arsene F, Tomoyasu T, Bukau B. The heat shock response of Escherichia coli. Int J Food Microbiol. 2000;55(1-3):3-9.
25. Kawate T, Gouaux E. Fluorescence-detection size-exclusion chromatography for precrystallization screening of integral membrane proteins. Structure. 2006;14(4):673-81.
26. Schlegel S, Rujas E, Ytterberg AJ, Zubarev RA, Luirink J, de Gier JW. Optimizing heterologous protein production in the periplasm of E. coli by regulating gene expression levels. Microb Cell Fact. 2013;12:24.
27. Drew DE, von Heijne G, Nordlund P, de Gier JW. Green fluorescent protein as an indicator to monitor membrane protein overexpression in Escherichia coli. FEBS Lett. 2001;507(2):220-4.
28. Froderberg L, Rohl T, van Wijk KJ, de Gier JW. Complementation of bacterial SecE by a chloroplastic homologue. FEBS Lett. 2001;498(1):52-6.
29. Drew D, Slotboom DJ, Friso G, Reda T, Genevaux P, Rapp M, Meindl-Beinker NM, Lambert W, Lerch M, Daley DO, et al. A scalable, GFP-based pipeline for membrane protein overexpression screening and purification. Protein Sci. 2005;14(8):2011-7.