Utility of Ion Mobility Mass Spectrometry for Drug-to-Antibody Ratio Measurements in Antibody-Drug Conjugates

Journal of the American Society for Mass Spectrometry

Volumn 26, Issue 10, 2015, Pages 1791-1794

  Huang, Richard Y C ^a   Deyanova, Ekaterina G ^a   Passmore, David ^a   Rangan, Vangipuram ^a   Deshpande, Shrikant ^a   Tymiak, Adrienne A ^a   Chen, Guodong ^a  

^a BRISTOL MYERS SQUIBB   (United States)

Author keywords

ADC; DAR; Ion mobility; MS

Indexed keywords

ANTIBODIES; CHEMICAL BONDS; COVALENT BONDS; ION MOBILITY SPECTROMETERS; IONS; MASS SPECTROMETRY; PROTEINS; SIGNAL TO NOISE RATIO; SPECTROMETRY;

ADC; ANTIBODY-DRUG CONJUGATES; DAR; HIGH HETEROGENEITY; ION MOBILITY; ION MOBILITY-MASS SPECTROMETRY; MS; PHARMACEUTICAL INDUSTRY;

ANALOG TO DIGITAL CONVERSION;

DRUG ANTIBODY; ANTIBODY CONJUGATE; DRUG;

ARTICLE; DEGLYCOSYLATION; DISULFIDE BOND; DRUG INDUSTRY; ION MOBILITY SPECTROMETRY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MEASUREMENT; SIGNAL NOISE RATIO; WORKFLOW; CHEMISTRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PROCEDURES;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; IMMUNOCONJUGATES; MASS SPECTROMETRY; PHARMACEUTICAL PREPARATIONS;

EID: 84941561397     PISSN: 10440305     EISSN: 18791123     Source Type: Journal    
DOI: 10.1007/s13361-015-1203-1     Document Type: Article

References (19)

1. Panowksi, S.; Bhakta, S.; Raab, H.; Polakis, P.; Junutula, J.R.: Site-specific antibody drug conjugates for cancer therapy. mAbs 6(1), 34-45 (2014)
2. Beck, A.; Wurch, T.; Bailly, C.; Corvaia, N.: Strategies and challenges for the next generation of therapeutic antibodies. Nat. Rev. Immunol. 10(5), 345-352 (2010)
3. Xu, K.; Liu, L.; Dere, R.; Mai, E.; Erickson, R.; Hendricks, A.; Lin, K.; Junutula, J.R.; Kaur, S.: Characterization of the drug-to-antibody ratio distribution for antibody-drug conjugates in plasma/serum. Bioanalysis 5(9), 1057-1071 (2013)
4. Haverick, M.; Mengisen, S.; Shameem, M.; Ambrogelly, A.L.: Separation of mAbs molecular variants by analytical hydrophobic interaction chromatography HPLC: overview and applications. mAbs 6(4), 852-858 (2014)
5. Wakankar, A.; Chen, Y.; Gokarn, Y.; Jacobson, F.S.: Analytical methods for physicochemical characterization of antibody drug conjugates. mAbs 3(2), 161-172 (2011)
6. Wagner-Rousset, E.; Janin-Bussat, M.-C.; Colas, O.; Excoffier, M.; Ayoub, D.; Haeuw, J.-F.; Rilatt, I.; Perez, M.; Corvaïa, N.; Beck, A.: Antibody-drug conjugate model fast characterization by LC-MS following IdeS proteolytic digestion. mAbs 6(1), 173-184 (2014)
7. Valliere-Douglass, J.F.; Hengel, S.M.; Pan, L.Y.: Approaches to interchain cysteine-linked ADC characterization by mass spectrometry. Mol. Pharm. 12(6), 1774-1783 (2015)
8. Xing, W.; Busino, L.; Hinds, T.R.; Marionni, S.T.; Saifee, N.H.; Bush, M.F.; Pagano, M.; Zheng, N.: SCFFBXL3 ubiquitin ligase targets cryptochromes at their cofactor pocket. Nature 496(7443), 64-68 (2013)
9. Mehmood, S.; Marcoux, J.; Hopper, J.T.S.; Allison, T.M.; Liko, I.; Borysik, A.J.; Robinson, C.V.: Charge reduction stabilizes intact membrane protein complexes for mass spectrometry. J. Am. Chem. Soc. 136(49), 17010-17012 (2014)
10. Politis, A.; Park, A.Y.; Hall, Z.; Ruotolo, B.T.; Robinson, C.V.: Integrative modeling coupled with ion mobility mass spectrometry reveals structural features of the clamp loader in complex with single-stranded DNA binding protein. J. Mol. Biol. 425(23), 4790-4801 (2013)
11. Jones, L.; Zhang, H.; Cui, W.; Kumar, S.; Sperry, J.; Carroll, J.; Gross, M.: Complementary MS methods assist conformational characterization of antibodies with altered S-S bonding networks. J. Am. Soc. Mass Spectrom. 24(6), 835-845 (2013)
12. Chen, G.; Warrack, B.M.; Goodenough, A.K.; Wei, H.; Wang-Iverson, D.B.; Tymiak, A.A.: Characterization of protein therapeutics by mass spectrometry: recent developments and future directions. Drug Discov. Today 16(1/2), 58-64 (2011)
13. Hyung, S.-J.; Feng, X.; Che, Y.; Stroh, J.; Shapiro, M.: Detection of conformation types of cyclosporin retaining intramolecular hydrogen bonds by mass spectrometry. Anal. Bioanal. Chem. 406(24), 5785-5794 (2014)
14. Chen, S.-H.; Chen, L.; Russell, D.H.: Metal-induced conformational changes of human metallothionein-2A: a combined theoretical and experimental study of metal-free and partially metalated intermediates. J. Am. Chem. Soc. 136(26), 9499-9508 (2014)
15. Iacob, R.E.; Murphy, J.P.; Engen, J.R.: Ion mobility adds an additional dimension to mass spectrometric analysis of solution-phase hydrogen/deuterium exchange. Rapid Commun. Mass Spectrom. 22(18), 2898-2904 (2008)
16. Debaene, F.; Bœuf, A.; Wagner-Rousset, E.; Colas, O.; Ayoub, D.; Corvaïa, N.; Van Dorsselaer, A.; Beck, A.; Cianférani, S.: Innovative native MS methodologies for antibody drug conjugate characterization: high resolution native MS and IM-MS for average DAR and DAR distribution assessment. Anal. Chem. 86(21), 10674-10683 (2014)
17. Reh, E.; Hahn, B.; Lamotte, S.: Evaluation of stationary phases for 2-dimensional HPLC of proteins: Part 1. Validation of commercial RP-columns. J. Chromatogr. B 844(2), 204-212 (2006)
18. Chan, C.-C.; Bolgar, M.S.; Dalpathado, D.; Lloyd, D.K.: Mitigation of signal suppression caused by the use of trifluoroacetic acid in liquid chromatography mobile phases during liquid chromatography/mass spectrometry analysis via post-column addition of ammonium hydroxide. Rapid Commun. Mass Spectrom. 26(12), 1507-1514 (2012)
19. Brady, L.J.; Valliere-Douglass, J.; Martinez, T.; Balland, A.: Molecular mass analysis of antibodies by on-line SEC-MS. J. Am. Soc. Mass Spectrom. 19(4), 502-509 (2008)