A luminescent assay for real-time measurements of receptor endocytosis in living cells

Analytical Biochemistry

Volumn 489, Issue , 2015, Pages 1-8

  Robers, Matthew B ^a   Binkowski, Brock F ^a   Cong, Mei ^a   Zimprich, Chad ^a   Corona, Cesear ^b   McDougall, Mark ^b   Otto, George ^a   Eggers, Christopher T ^a   Hartnett, Jim ^a   Machleidt, Thomas ^a   Fan, Frank ^a   Wood, Keith V ^a  

^a PROMEGA CORPORATION   (United States)

^b Promega Biosciences   (United States)

Author keywords

Endocytosis; G protein coupled receptor; Internalization; NanoLuc; Receptor tyrosine kinase

Indexed keywords

AMINO ACIDS; CELL MEMBRANES; LIGANDS; PROTEINS;

ENDOCYTOSIS; G PROTEIN COUPLED RECEPTORS; INTERNALIZATION; NANOLUC; RECEPTOR TYROSINE KINASE;

MOLECULAR BIOLOGY;

EPIDERMAL GROWTH FACTOR RECEPTOR; G PROTEIN COUPLED RECEPTOR; LUCIFERASE; ARTHROPOD PROTEIN; FLUORESCENT DYE; HYBRID PROTEIN; INTERLEUKIN 6; LIGAND; PEPTIDE FRAGMENT; SIGNAL PEPTIDE;

ARTICLE; BIOLUMINESCENCE; CELL TRACKING; CONTROLLED STUDY; EMBRYO; ENDOCYTOSIS; GENE FUSION; HEK293 CELL LINE; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; LUMINESCENCE; LUMINESCENT ASSAY; PRIORITY JOURNAL; REAL TIME TRACKING SYSTEM; WORKFLOW; AGONISTS; ANIMAL; CELL MEMBRANE; CHEMISTRY; CONFOCAL MICROSCOPY; DRUG DEVELOPMENT; DRUG EFFECTS; ENZYMOLOGY; FLUORESCENCE MICROSCOPY; GENETICS; KINETICS; METABOLISM; PROCEDURES; REPORTER GENE; VALIDATION STUDY;

ANIMALS; ARTHROPOD PROTEINS; CELL MEMBRANE; DRUG DISCOVERY; ENDOCYTOSIS; FLUORESCENT DYES; GENES, REPORTER; HEK293 CELLS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; INTERLEUKIN-6; KINETICS; LIGANDS; LUCIFERASES; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; PEPTIDE FRAGMENTS; PROTEIN SORTING SIGNALS; RECEPTORS, G-PROTEIN-COUPLED; RECOMBINANT FUSION PROTEINS;

EID: 84941090046     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2015.08.005     Document Type: Article

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