Quantitative measurement of cell membrane receptor internalization by the nanoluciferase reporter: Using the G protein-coupled receptor RXFP3 as a model

Biochimica et Biophysica Acta - Biomembranes

Volumn 1848, Issue 2, 2015, Pages 688-694

  Liu, Yu ^a,b   Song, Ge ^a   Shao, Xiao Xia ^a   Liu, Ya Li ^b   Guo, Zhan Yun ^a,b  

^a TONGJI UNIVERSITY   (China)

^b TONGJI UNIVERSITY SCHOOL OF MEDICINE   (China)

Author keywords

Bioluminescence; GPCR; Internalization; Receptor; Relaxin 3; RXFP3

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; G PROTEIN COUPLED RECEPTOR; G PROTEIN COUPLED RECEPTOR RXFP3; MEMBRANE RECEPTOR; NANOLUCIFERASE; NANOPARTICLE; RELAXIN 3; UNCLASSIFIED DRUG; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; LIGAND; LUCIFERASE; RELAXIN; RLN3 PROTEIN, HUMAN; RXFP3 PROTEIN, HUMAN;

ARTICLE; BIOLUMINESCENCE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; FLUORESCENCE MICROSCOPY; HEK293 CELL LINE; INTRACELLULAR TRANSPORT; LIGAND BINDING; LUCIFERASE ASSAY; PH; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; WILD TYPE; BIOASSAY; CELL MEMBRANE; CHEMISTRY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; HUMAN; KINETICS; METABOLISM; PROTEIN TRANSPORT; REPORTER GENE; STAINING;

BIOLOGICAL ASSAY; CELL MEMBRANE; ESCHERICHIA COLI; GENE EXPRESSION; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; HEK293 CELLS; HUMANS; HYDROGEN-ION CONCENTRATION; KINETICS; LIGANDS; LUCIFERASES; MICROSCOPY, FLUORESCENCE; PROTEIN TRANSPORT; RECEPTORS, G-PROTEIN-COUPLED; RECOMBINANT FUSION PROTEINS; RELAXIN; STAINING AND LABELING;

EID: 84919363188     PISSN: 00052736     EISSN: 18792642     Source Type: Journal    
DOI: 10.1016/j.bbamem.2014.11.026     Document Type: Article

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