Fine-tuning of photoautotrophic protein production by combining promoters and neutral sites in the cyanobacterium Synechocystis sp. strain PCC 6803

Applied and Environmental Microbiology

Volumn 81, Issue 19, 2015, Pages 6857-6863

  Ng, Andrew H ^a   Berla, Bertram M ^b   Pakrasi, Himadri B ^a,b  

^a WASHINGTON UNIVERSITY   (United States)

^b WASHINGTON UNIVERSITY IN ST LOUIS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHROMOSOMES; DNA; ESCHERICHIA COLI; PROTEINS; SOLAR ENERGY; YEAST;

CYANOBACTERIUM SYNECHOCYSTIS; ENHANCED YELLOW FLUORESCENT PROTEINS; EXPRESSION CASSETTE; HETEROLOGOUS GENES; PROTEIN PRODUCTION; SHUTTLE VECTOR; SYNTHETIC BIOLOGY; TRANSCRIPTOMES;

METABOLIC ENGINEERING;

CARBON DIOXIDE; CHROMOSOME; CYANOBACTERIUM; FECAL COLIFORM; GENE EXPRESSION; GENETIC ENGINEERING; HETEROTROPHY; PHOTOAUTOTROPHY; PLASMID; PROTEIN; SOLAR POWER;

CYANOBACTERIA; ESCHERICHIA COLI; SACCHAROMYCES CEREVISIAE; SYNECHOCYSTIS SP.;

BACTERIAL PROTEIN;

AUTOTROPHY; GENE EXPRESSION REGULATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; LIGHT; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLASMID; PROMOTER REGION; RADIATION RESPONSE; SYNECHOCYSTIS;

AUTOTROPHIC PROCESSES; BACTERIAL PROTEINS; BASE SEQUENCE; GENE EXPRESSION REGULATION, BACTERIAL; LIGHT; MOLECULAR SEQUENCE DATA; PLASMIDS; PROMOTER REGIONS, GENETIC; SYNECHOCYSTIS;

EID: 84940783269     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01349-15     Document Type: Article

References (43)

1. Berla BM, Saha R, Immethun CM, Maranas CD, Moon TS, Pakrasi HB. 2013. Synthetic biology of cyanobacteria: unique challenges and opportunities. Front Microbiol 4:246. http://dx.doi.org/10.3389/fmicb.2013.00246.
2. Heidorn T, Camsund D, Huang HH, Lindberg P, Oliveira P, Stensjö K, Lindblad P. 2011. Synthetic biology in cyanobacteria: engineering and analyzing novel functions. Methods Enzymol 497:539-579. http://dx.doi.org/10.1016/B978-0-12-385075-1.00024-X.
3. Oliver JWK, Atsumi S. 2014. Metabolic design for cyanobacterial chemical synthesis. Photosynth Res 120:249-261. http://dx.doi.org/10.1007/s11120-014-9997-4.
4. Wang B, Wang J, Zhang W, Meldrum DR. 2012. Application of synthetic biology in cyanobacteria and algae. Front Microbiol 3:344. http://dx.doi.org/10.3389/fmicb.2012.00344.
5. Gronenberg LS, Marcheschi RJ, Liao JC. 2013. Next generation biofuel engineering in prokaryotes. Curr Opin Chem Biol 17:462-471. http://dx.doi.org/10.1016/j.cbpa.2013.03.037.
6. Ducat DC, Way JC, Silver PA. 2011. Engineering cyanobacteria to generate high-value products. Trends Biotechnol 29:95-103. http://dx.doi.org/10.1016/j.tibtech.2010.12.003.
7. Varman AM, Yu Y, You L, Tang YJ. 2013. Photoautotrophic production of D-lactic acid in an engineered cyanobacterium. Microb Cell Fact 12:117. http://dx.doi.org/10.1186/1475-2859-12-117.
8. Kaneko T, Nakamura Y, Sasamoto S, Watanabe A, Kohara M, Matsumoto M, Shimpo S, Yamada M, Tabata S. 2003. Structural analysis of four large plasmids harboring in a unicellular cyanobacterium, Synechocystis sp. PCC 6803. DNA Res 10:221-228. http://dx.doi.org/10.1093/dnares/10.5.221.
9. Yang X, McFadden BA. 1993. A small plasmid, pCA2.4, from the cyanobacterium Synechocystis sp. strain PCC 6803 encodes a Rep protein and replicates by a rolling circle mechanism. J Bacteriol 175:3981-3991.
10. Yang X, McFadden BA. 1994. The complete DNA sequence and replication analysis of the plasmid pCB2.4 from the cyanobacterium Synechocystis PCC 6803. Plasmid 31:131-137. http://dx.doi.org/10.1006/plas.1994.1014.
11. Xu W, McFadden BA. 1997. Sequence analysis of plasmid pCC5.2 from cyanobacterium Synechocystis PCC 6803 that replicates by a rolling circle mechanism. Plasmid 37:95-104. http://dx.doi.org/10.1006/plas.1997.1281.
12. Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW. 29 July 2014. Broad-host-range vector system for synthetic biology and biotechnology in cyanobacteria. Nucleic Acids Res http://dx.doi.org/10.1093/nar/gku673.
13. Xu Y, Alvey RM, Byrne PO, Graham JE, Shen G, Bryant DA. 2011. Expression of genes in cyanobacteria: adaptation of endogenous plasmids as platforms for high-level gene expression in Synechococcus sp. PCC 7002. Methods Mol Biol 684:273-293. http://dx.doi.org/10.1007/978-1-60761-925-3_21.
14. Berla BM, Pakrasi HB. 2012. Upregulation of plasmid genes during stationary phase in Synechocystis sp. strain PCC 6803, a cyanobacterium. Appl Environ Microbiol 78:5448-5451. http://dx.doi.org/10.1128/AEM.01174-12.
15. Markley AL, Begemann MB, Clarke RE, Gordon GC, Pfleger BF. 25 September 2014. Synthetic biology toolbox for controlling gene expression in the cyanobacterium Synechococcus sp. strain PCC 7002. ACS Synth Biol http://dx.doi.org/10.1021/sb500260k.
16. Huang H-H, Camsund D, Lindblad P, Heidorn T. 2010. Design and characterization of molecular tools for a synthetic biology approach towards developing cyanobacterial biotechnology. Nucleic Acids Res 38:2577-2593. http://dx.doi.org/10.1093/nar/gkq164.
17. Imamura S, Asayama M. 2009. Sigma factors for cyanobacterial transcription. Gene Regul Syst Biol 3:65-87.
18. Ma J, Campbell A, Karlin S. 2002. Correlations between Shine-Dalgarno sequences and gene features such as predicted expression levels and operon structures. J Bacteriol 184:5733-5745. http://dx.doi.org/10.1128/JB.184.20.5733-5745.2002.
19. Griese M, Lange C, Soppa J. 2011. Ploidy in cyanobacteria. FEMS Microbiol Lett 323:124-131. http://dx.doi.org/10.1111/j.1574-6968.2011.02368.x.
20. Murphy RC, Bryant DA, Porter RD, de Marsac NT. 1987. Molecular cloning and characterization of the recA gene from the cyanobacterium Synechococcus sp. strain PCC 7002. J Bacteriol 169:2739-2747.
21. Dong G, Golden SS. 2008. How a cyanobacterium tells time. Curr Opin Microbiol 11:541-546. http://dx.doi.org/10.1016/j.mib.2008.10.003.
22. Beck C, Hertel S, Rediger A, Lehmann R, Wiegard A, Kölsch A, Heilmann B, Georg J, Hess WR, Axmann IM. 2014. Daily expression pattern of protein-encoding genes and small noncoding RNAs in Synechocystis sp. strain PCC 6803. Appl Environ Microbiol 80:5195-5206. http://dx.doi.org/10.1128/AEM.01086-14.
23. Landry BP, Stöckel J, Pakrasi HB. 2013. Use of degradation tags to control protein levels in the cyanobacterium Synechocystis sp. strain PCC 6803. Appl Environ Microbiol 79:2833-2835. http://dx.doi.org/10.1128/AEM.03741-12.
24. Daniel R, Rubens JR, Sarpeshkar R, Lu TK. 2013. Synthetic analog computation in living cells. Nature 497:619-623. http://dx.doi.org/10.1038/nature12148.
25. Moon TS, Lou C, Tamsir A, Stanton BC, Voigt CA. 2012. Genetic programs constructed from layered logic gates in single cells. Nature 491: 249-253. http://dx.doi.org/10.1038/nature11516.
26. Stricker J, Cookson S, Bennett MR, Mather WH, Tsimring LS, Hasty J. 2008. Afast, robust and tunable synthetic gene oscillator. Nature 456:516-519. http://dx.doi.org/10.1038/nature07389.
27. Camsund D, Heidorn T, Lindblad P. 2014. Design and analysis of LacIrepressed promoters and DNA-looping in a cyanobacterium. J Biol Eng 8:4. http://dx.doi.org/10.1186/1754-1611-8-4.
28. Huang H-H, Lindblad P. 2013. Wide-dynamic-range promoters engineered for cyanobacteria. J Biol Eng 7:10. http://dx.doi.org/10.1186/1754-1611-7-10.
29. Rosano GL, Ceccarelli EA. 2014. Recombinant protein expression in Escherichia coli: advances and challenges. Front Microbiol 5:172. http://dx.doi.org/10.3389/fmicb.2014.00172.
30. Varman AM, Xiao Y, Pakrasi HB, Tang YJ. 2013. Metabolic engineering of Synechocystis sp. strain PCC 6803 for isobutanol production. Appl Environ Microbiol 79:908-914. http://dx.doi.org/10.1128/AEM.02827-12.
31. Xue Y, Zhang Y, Cheng D, Daddy S, He Q. 2014. Genetically engineering Synechocystis sp. Pasteur Culture Collection 6803 for the sustainable production of the plant secondary metabolite p-coumaric acid. Proc Natl Acad Sci U S A 111:9449-9454. http://dx.doi.org/10.1073/pnas.1323725111.
32. Marbouty M, Mazouni K, Saguez C, Cassier-Chauvat C, Chauvat F. 2009. Characterization of the Synechocystis strain PCC 6803 penicillinbinding proteins and cytokinetic proteins FtsQ and FtsW and their network of interactions with ZipN. J Bacteriol 191:5123-5133. http://dx.doi.org/10.1128/JB.00620-09.
33. 2. Metab Eng 16:68-77. http://dx.doi.org/10.1016/j.ymben.2013.01.001.
34. Mitschke J, Georg J, Scholz I, Sharma CM, Dienst D, Bantscheff J, Voss B, Steglich C, Wilde A, Vogel J, Hess WR. 2011. An experimentally anchored map of transcriptional start sites in the model cyanobacterium Synechocystis sp. PCC 6803. Proc Natl Acad Sci U S A 108:2124-2129. http://dx.doi.org/10.1073/pnas.1015154108.
35. Zhou J, Zhang H, Meng H, Zhu Y, Bao G, Zhang Y, Li Y, Ma Y. 2014. Discovery of a super-strong promoter enables efficient production of heterologous proteins in cyanobacteria. Sci Rep 4:4500. http://dx.doi.org/10.1038/srep04500.
36. Liu X, Sheng J, Curtiss R. 2011. Fatty acid production in genetically modified cyanobacteria. Proc Natl Acad Sci U S A 108:6899-6904. http://dx.doi.org/10.1073/pnas.1103014108.
37. Mohamed A, Jansson C. 1989. Influence of light on accumulation of photosynthesis-specific transcripts in the cyanobacterium Synechocystis 6803. Plant Mol Biol 13:693-700. http://dx.doi.org/10.1007/BF00016024.
38. Singh AK, Elvitigala T, Cameron JC, Ghosh BK, Bhattacharyya-Pakrasi M, Pakrasi HB. 2010. Integrative analysis of large scale expression profiles reveals core transcriptional response and coordination between multiple cellular processes in a cyanobacterium. BMC Syst Biol 4:105. http://dx.doi.org/10.1186/1752-0509-4-105.
39. Rutherford K, Parkhill J, Crook J, Horsnell T, Rice P, Rajandream MA, Barrell B. 2000. Artemis: sequence visualization and annotation. Bioinformatics 16:944-945. http://dx.doi.org/10.1093/bioinformatics/16.10.944.
40. Rippka R, Deruelles J, Waterbury JB, Herdman M, Stanier RY. 1979. Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 111:1-61. http://dx.doi.org/10.1099/00221287-111-1-1.
41. Quan J, Tian J. 2011. Circular polymerase extension cloning for highthroughput cloning of complex and combinatorial DNA libraries. Nat Protoc 6:242-251. http://dx.doi.org/10.1038/nprot.2010.181.
42. Tsinoremas NF, Kutach AK, Strayer CA, Golden SS. 1994. Efficient gene transfer in Synechococcus sp. strains PCC 7942 and PCC 6301 by interspecies conjugation and chromosomal recombination. J Bacteriol 176:6764-6768.
43. 2 conversion to ethylene. Nat Plants 1:15053. http://dx.doi.org/10.1038/nplants.2015.53.