A flow cytometry-based method to simplify the analysis and quantification of protein association to chromatin in mammalian cells

Nature Protocols

Volumn 10, Issue 9, 2015, Pages 1297-1307

  Forment, Josep V ^a   Jackson, Stephen P ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

5 ETHYNYL 2' DEOXYURIDINE; HISTONE H2AX; HISTONE H3; NUCLEOTIDE DERIVATIVE; REPLICATION FACTOR A; UNCLASSIFIED DRUG; CHROMATIN; PROTEIN;

ANIMAL CELL; ANTIBODY SPECIFICITY; ARTICLE; CELL CULTURE; CELL CYCLE G1 PHASE; CELL CYCLE G2 PHASE; CELL CYCLE S PHASE; CELLULAR DISTRIBUTION; CHROMATIN; CONTROLLED STUDY; CYTOSKELETON; DNA CONTENT; DNA REPLICATION; DOUBLE STRANDED DNA BREAK; EMBRYO; FLOW CYTOMETRY; FRACTIONATION; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE MICROSCOPY; IMMUNOHISTOCHEMISTRY; INTERMETHOD COMPARISON; LIMIT OF DETECTION; LIMIT OF QUANTITATION; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; PROCESS DEVELOPMENT; PROTEIN ANALYSIS; PROTEIN LOCALIZATION; PROTEIN TARGETING; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; SINGLE CELL ANALYSIS; WESTERN BLOTTING; CHEMISTRY; FLUORESCENCE MICROSCOPY;

CELLS, CULTURED; CHROMATIN; FLOW CYTOMETRY; MICROSCOPY, FLUORESCENCE; PROTEINS;

EID: 84940480170     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2015.066     Document Type: Article

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