A novel method based on click chemistry, which overcomes limitations of cell cycle analysis by classical determination of BrdU incorporation, allowing multiplex antibody staining

Cytometry Part A

Volumn 73, Issue 7, 2008, Pages 626-636

  Cappella, Paolo ^a   Gasparri, Fabio ^a   Pulici, Maurizio ^a   Moll, Jürgen ^a  

^a NERVIANO MEDICAL SCIENCES   (Italy)

Author keywords

BDA; BMA; BrdU; Cell cycle; Click chemistry; EdU; High content analysis; Multiplex analysis

Indexed keywords

5 ETHYNYLDEOXYURIDINE; ALKYNE; ALKYNYL GROUP; ANTIBODY; AZIDE; BROXURIDINE; CASPASE 3; COPPER; DEOXYURIDINE DERIVATIVE; EPITOPE; HISTONE H3; MONOCLONAL ANTIBODY; NUCLEOTIDE;

ANALYTIC METHOD; ANIMAL CELL; ARTICLE; CATALYSIS; CELL CYCLE; CELL PROLIFERATION; CONTROLLED STUDY; CYCLOADDITION; DERIVATIZATION; DNA DENATURATION; FEASIBILITY STUDY; FLUORESCENCE; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; SENSITIVITY AND SPECIFICITY; SIGNAL NOISE RATIO; STAINING;

ANIMALS; ANTIBODIES; BONE MARROW CELLS; BROMODEOXYURIDINE; CASPASE 3; CELL CYCLE; DEOXYURIDINE; FLOW CYTOMETRY; HISTONES; HL-60 CELLS; HUMANS; MALE; MICE; MICE, INBRED BALB C; MODELS, BIOLOGICAL;

EID: 46449118537     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.20582     Document Type: Article

References (29)

1. Gasparri F, Cappella P, Galvani A. Multiparametric cell cycle analysis by automated microscopy. J Biomol Screen 2006;11:586-598.
2. Krutzik PO, Crane JM, Clutter MR, Nolan GP. High-content single-cell drug screening with phosphospecific flow cytometry. Nat Chem Biol 2008;4:132-142.
3. Perfetto SP, Chattopadhyay PK, Roederer M. Seventeen-colour flow cytometry: unravelling the immune system. Nat Rev Immunol 2004;4:648-655.
4. Mittag A, Lenz D, Gerstner AO, Tárnok A. Hyperchromatic cytometry principles for cytomics using slide based cytometry. Cytometry A 2006;69A:691-703.
5. Tárnok A, Bocsi J, Brockhoff G. Cytomics - Importance of multimodal analysis of cell function and proliferation in oncology. Cell Prolif 2006;39:495-505.
6. Leif RC, Stein JH, Zucker RM. A short history of the initial application of anti-5-BrdU to the detection and measurement of S phase. Cytometry A 2004;58A:45-52.
7. Terry NH, White RA. Cell cycle kinetics estimated by analysis of bromodeoxyuridine incorporation. Methods Cell Biol 2001;63:355-374.
8. Gonchoroff NJ, Katzmann JA, Currie RM, Evans EL, Houck DW, Kline BC, Greipp PR, Loken MR. S-phase detection with an antibody to bromodeoxyuridine. Role of DNase pretreatment. J Immunol Methods 1986;93:97-101.
9. Hammers HJ, Kirchner H, Schlenke P. Ultraviolet-induced detection of halogenated pyrimidines: Simultaneous analysis of DNA replication and cellular markers. Cytometry 2000;40:327-335.
10. Tkatchenko AV. Whole-mount BrdU staining of proliferating cells by DNase treatment: Application to postnatal mammalian retina. Biotechniques 2006;40:29-32.
11. Tang X, Falls DL, Li X, Lane T, Luskin MB. Antigen-retrieval procedure for bromodeoxyuridine immunolabeling with concurrent labeling of nuclear DNA and antigens damaged by HCl pretreatment. J Neurosci 2007;27:5837-5844.
12. Jacobberger JW, Frisa PS, Sramkoski RM, Stefan T, Shults KE, Soni DV. A new biomarker for mitotic cells. Cytometry Part A 2008;73A:5-15.
13. Deiters A, Cropp TA, Mukherji M, Chin JW, Anderson JC, Schultz PG. Adding amino acids with novel reactivity to the genetic code of Saccharomyces cerevisiae. J Am Chem Soc 2003;125:11782-11783.
14. Seo TS, Li Z, Ruparel H, Ju J. Click chemistry to construct fluorescent oligonucleotides for DNA sequencing. J Org Chem 2003;68:609-612.
15. Rostovtsev VV, Green LG, Fokin VV, Sharpless KB. A stepwise huisgen cycloaddition process: Copper(I)-catalyzed regioselective "ligation" of azides and terminal alkynes. Angew Chem Int Ed Engl 2002;41:2596-2599.
16. Lin TS, Neenan JP, Cheng YC, Prusoff WH. Synthesis and antiviral activity of 5- and 5′-substituted thymidine analogs. J Med Chem 1976;19:495-498.
17. Pochet S, Dugue L, Labesse G, Delepierre M, Munier-Lehmann H. Comparative study of purine and pyrimidine nucleoside analogues acting on the thymidylate kinases of Mycobacterium tuberculosis and of humans. Chembiochem 2003;4:742-747.
18. Andreotti PE, Cree IA, Kurbacher CM, Hartmann DM, Linder D, Harel G, Gleiberman I, Caruso PA, Ricks SH, Untch M. Chemosensitivity testing of human tumors using a microplate adenosine triphosphate luminescence assay: Clinical correlation for cisplatin resistance of ovarian carcinoma. Cancer Res 1995;55:5276-5282.
19. Breinbauer R, Kohn M. Azide-alkyne coupling: A powerful reaction for bioconjugate chemistry. Chembiochem 2003;4:1147-1149.
20. Kolb HC, Sharpless KB. The growing impact of click chemistry on drug discovery. Drug Discov Today 2003;8:1128-1137.
21. Balzarini J, De Clercq E, Ayusawa D, Seno T. Thymidylate synthetase-deficient mouse FM3A mammary carcinoma cell line as a tool for studying the thymidine salvage pathway and the incorporation of thymidine analogues into host cell DNA. Biochem J 1984;217:245-252.
22. Cristofoli WA, Wiebe LI, De Clercq E, Andrei G, Snoeck R, Balzarini J, Knaus EE. 5-alkynyl analogs of arabinouridine and 2′-deoxyuridine: Cytostatic activity against herpes simplex virus and varicella-zoster thymidine kinase gene-transfected cells. J Med Chem 2007;50:2851-2857.
23. Meneni S, Ott I, Sergeant CD, Sniady A, Gust R, Dembinski R. 5-Alkynyl-2′-deoxyuridines: Chromatography-free synthesis and cytotoxicity evaluation against human breast cancer cells. Bioorg Med Chem 2007;15:3082-3088.
24. Gierlich J, Gutsmiedl K, Gramlich PM, Schmidt A, Burley GA, Carell T. Synthesis of highly modified DNA by a combination of PCR with alkyne-bearing triphosphates and click chemistry. Chemistry 2007;13:9486-9494.
25. Rodionov VO, Fokin VV, Finn MG. Mechanism of the ligand-free Cul-catalyzed azide-alkyne cycloaddition reaction. Angew Chem Int Ed Engl 2005;44:2210-2215.
26. Hsu TL, Hanson SR, Kishikawa K, Wang SK, Sawa M, Wong CH. Alkynyl sugar analogs for the labeling and visualization of glycoconjugates in cells. Proc Natl Acad Sci USA 2007;104:2614-2619.
27. Baskin JM, Prescher JA, Laughlin ST, Agard NJ, Chang PV, Miller IA, Lo A, Codelli JA, Bertozzi CR. Copper-free click chemistry for dynamic in vivo imaging. Proc Natl Acad Sci USA 2007;104:16793-16797.
28. Young DW, Bender A, Hoyt J, McWhinnie E, Chirn GW, Tao CY, Tallarico JA, Labow M, Jenkins JL, Mitchison TJ, Feng Y. Integrating high-content screening and ligand-target prediction to identify mechanism of action. Nat Chem Biol 2008;4:59-68.
29. Salic A, Mitchison TJ. A chemical method for fast and sensitive detection of DNA synthesis in vivo. Proc Natl Acad Sci USA 2008;105:2415-2420.