A chemical method for fast and sensitive detection of DNA synthesis in vivo

Proceedings of the National Academy of Sciences of the United States of America

Volumn 105, Issue 7, 2008, Pages 2415-2420

  Salic, Adrian ^a   Mitchison, Timothy J ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

BrdU; Click chemistry; DNA replication; EdU; Microscopy

Indexed keywords

DNA; EDOXUDINE; 5 ETHYNYLDEOXYURIDINE; 5-ETHYNYL-2'-DEOXYURIDINE; DEOXYURIDINE; DRUG DERIVATIVE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CELL PROLIFERATION; CYCLOADDITION; DNA DENATURATION; DNA REPLICATION; DNA SYNTHESIS; MICROSCOPY; MOUSE; NONHUMAN; PRIORITY JOURNAL; ANIMAL; BIOSYNTHESIS; CELL STRAIN 3T3; CHEMICAL STRUCTURE; CHEMISTRY; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; TIME;

ANIMALIA;

ANIMALS; DEOXYURIDINE; DNA; DNA REPLICATION; MICE; MOLECULAR STRUCTURE; NIH 3T3 CELLS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; TIME FACTORS;

EID: 40649111377     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0712168105     Document Type: Article

References (18)

1. Bick MD, Davidson RL (1974) Total substitution of bromodeoxyuridine for thymidine in the DNA of a bromodeoxyuridine-dependent cell line. Proc Natl Acad Sci USA 71:2082-2086.
2. Gratzner HG (1982) Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: A new reagent for detection of DNA replication. Science 218:474-475.
3. 3H thymidine labeling in human breast tumors. Mod Pathol 4:718-722.
4. Rakic P (2002) Neurogenesis in adult primate neocortex: an evaluation of the evidence. Nat Rev Neurosci 3:65-71.
5. Tornoe CW, et al. (2002) Peptidotriazoles on solid phase: [1,2,3]-Triazoles by regiospecific copper(i) -catalyzed 1,3-dipolar cycloadditions of terminal alkynes to azides. J Org Chem 67:3057-3064.
6. Rostovtsev VV, et al. (2002) A stepwise Huisgen cycloaddition process: copper(I)-catalyzed regioselective "ligation" of azides and terminal alkynes. Angew Chem Int Ed Engl 41:2596-2599.
7. De Clercq E, et al. (1979) (E)-5-(2-Bromovinyl)-2′- deoxyuridine: A potent and selective anti-herpes agent. Proc Natl Acad Sci USA 76:2947-2951.
8. Walker RT, et al. (1980) The synthesis and properties of some 5-substituted uracil derivatives. Nucleic Acids Symp Ser s95-s102.
9. Chan TR, et al. (2004) Polytriazoles as copper(I)-stabilizing ligands in catalysis. Org Lett 6:2853-2855.
10. Rodionov VO, et al. (2005) Mechanism of the ligand-free CuI-catalyzed azide-alkyne cycloaddition reaction. Angew Chem Int Ed Engl 44:2210-2215.
11. Sunthankar P, et al. (1998). Synthesis of 5-azido-UDP-N- acetylhexosamine photoaffinity analogs and radiolabeled UDP-N-acetylhexosamines. Anal Biochem 258:195-201.
12. Speers AE, Cravatt BF (2004) Profiling enzyme activities in vivo using click chemistry methods. Chem Biol 11:535-546.
13. Deerinck TJ, et al. (1994) Fluorescence photooxidation with eosin: a method for high resolution immunolocalization and in situ hybridization detection for light and electron microscopy. J Cell Biol 126:901-910.
14. Betzig E, et al. (2006) Imaging intracellular fluorescent proteins at nanometer resolution. Science 313:1642-1645.
15. Maddox PS, et al. (2003) Spinning disk confocal microscope system for rapid high-resolution, multimode, fluorescence speckle microscopy and green fluorescent protein imaging in living cells. Methods Enzymol 360:597-617.
16. Sawin KE, Mitchison TJ (1991) Mitotic spindle assembly by two different pathways in vitro. J Cell Biol 112:925-940.
17. Yu CS, Oberdorfer F (2000) Synthesis of (E)-5-[2-(tri-n-butylstannyl) vinyl] substituted 2′-deoxyuridine derivatives for use in halogenation and radiohalogenation reactions. Synlett 1:86-88.
18. Zuckermann RN, et al. (1994) Discovery of nanomolar ligands for 7-transmembrane G-protein-coupled receptors from a diverse N-(substituted) glycine peptoid library. J Med Chem 37:2678-2685.