Simultaneous impairment of mitochondrial fission and fusion reduces mitophagy and shortens replicative lifespan

Scientific Reports

Volumn 5, Issue , 2015, Pages

  Bernhardt, Dominik ^a   Müller, Matthias ^a   Reichert, Andreas S ^a,b   Osiewacz, Heinz D ^a  

^a GOETHE UNIVERSITY   (Germany)

^b UNIVERSITY HOSPITAL DÜSSELDORF   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DNM1 PROTEIN, S CEREVISIAE; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE TRIPHOSPHATASE; MGM1 PROTEIN, S CEREVISIAE; MITOCHONDRIAL PROTEIN; SACCHAROMYCES CEREVISIAE PROTEIN;

AUTOPHAGY; CELL AGING; GENETICS; METABOLISM; MITOCHONDRIAL DYNAMICS; MITOCHONDRION; MITOPHAGY; SACCHAROMYCES CEREVISIAE;

AUTOPHAGY; CELL AGING; GTP PHOSPHOHYDROLASES; GTP-BINDING PROTEINS; MITOCHONDRIA; MITOCHONDRIAL DEGRADATION; MITOCHONDRIAL DYNAMICS; MITOCHONDRIAL PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 84940430015     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep07885     Document Type: Article

References (41)

1. Okamoto, K., Shaw, J. M. Mitochondrial morphology and dynamics in yeast and multicellular eukaryotes. Annu. Rev. Genet. 39, 503-536 (2005).
2. Westermann, B. Mitochondrial dynamics in model organisms: what yeasts, worms and flies have taught us about fusion and fission of mitochondria. Semin. Cell Dev. Biol. 21, 542-549 (2010).
3. Hermann, G. J. et al. Mitochondrial fusion in yeast requires the transmembrane GTPase Fzo1p. J. Cell Biol. 143, 359-373 (1998).
4. Jones, B. A., Fangman,W. L.Mitochondrial DNA maintenance in yeast requires a protein containing a region related to the GTP-binding domain of dynamin. Genes Dev. 6, 380-389 (1992).
5. Sesaki, H., Jensen, R. E. UGO1 encodes an outer membrane protein required for mitochondrial fusion. J. Cell Biol. 152, 1123-1134 (2001).
6. Rojo, M., Legros, F., Chateau, D., Lombes, A. Membrane topology and mitochondrial targeting of mitofusins, ubiquitous mammalian homologs of the transmembrane GTPase Fzo. J. Cell Sci. 115, 1663-1674 (2002).
7. Anton, F. et al. Ugo1 and Mdm30 act sequentially during Fzo1-mediated mitochondrial outer membrane fusion. J. Cell Sci. 124, 1126-1135 (2011).
8. Sesaki, H., Jensen, R. E. Ugo1p links the Fzo1p and Mgm1p GTPases for mitochondrial fusion. J. Biol. Chem. 279, 28298-28303 (2004).
9. Herlan, M., Vogel, F., Bornhovd, C., Neupert, W., Reichert, A. S. Processing of Mgm1 by the rhomboid-type protease Pcp1 is required for maintenance of mitochondrial morphology and of mitochondrial DNA. J. Biol. Chem. 278, 27781-27788 (2003).
10. Hoppins, S., Lackner, L., Nunnari, J. The machines that divide and fuse mitochondria. Annu. Rev. Biochem. 76, 751-780 (2007).
11. Abutbul-Ionita, I., Rujiviphat, J., Nir, I., McQuibban, G. A., Danino, D. Membrane tethering and nucleotide-dependent conformational changes drive mitochondrial genome maintenance (Mgm1) protein-mediated membrane fusion. J. Biol. Chem. 287, 36634-36638 (2012).
12. Sesaki, H., Southard, S. M., Yaffe, M. P.,Jensen, R. E. Mgm1p, a dynamin-related GTPase, is essential for fusion of the mitochondrial outer membrane. Mol. Biol. Cell 14, 2342-2356 (2003).
13. Shepard, K. A., Yaffe, M. P. The yeast dynamin-like protein, Mgm1p, functions on the mitochondrial outer membrane to mediate mitochondrial inheritance. J. Cell Biol. 144, 711-720 (1999).
14. Wong, E. D. et al. The dynamin-related GTPase, Mgm1p, is an intermembrane space protein required for maintenance of fusion competent mitochondria. J. Cell Biol. 151, 341-352 (2000).
15. Dohm, J. A., Lee, S. J., Hardwick, J. M., Hill, R. B., Gittis, A. G. Cytosolic domain of the human mitochondrial fission protein fis1 adopts a TPR fold. Proteins 54, 153-156 (2004).
16. Otsuga, D. et al. The dynamin-related GTPase, Dnm1p, controls mitochondrial morphology in yeast. J. Cell Biol. 143, 333-349 (1998).
17. Mozdy, A. D., McCaffery, J. M., Shaw, J. M. Dnm1p GTPase-mediated mitochondrial fission is a multi-step process requiring the novel integral membrane component Fis1p. J. Cell Biol. 151, 367-380 (2000).
18. Fekkes, P., Shepard, K. A., Yaffe, M. P. Gag3p, an outer membrane protein required for fission of mitochondrial tubules. J. Cell Biol. 151, 333-340 (2000).
19. Tieu, Q., Okreglak, V., Naylor, K., Nunnari, J. TheWD repeat protein, Mdv1p, functions as a molecular adaptor by interacting with Dnm1p and Fis1p during mitochondrial fission. J. Cell Biol. 158, 445-452 (2002).
20. Knorre, D. A., Popadin, K. Y., Sokolov, S. S.,Severin, F. F. Roles of mitochondrial dynamics under stressful and normal conditions in yeast cells. Oxid. Med. Cell Longev. 2013, 139491 (2013).
21. Westermann, B. Mitochondrial membrane fusion. Biochim. Biophys. Acta 1641, 195-202 (2003).
22. Ono, T., Isobe, K., Nakada, K., Hayashi, J. I. Human cells are protected from mitochondrial dysfunction by complementation of DNA products in fused mitochondria. Nat. Genet. 28, 272-275 (2001).
23. Chen, H., Chan, D. C. Mitochondrial dynamics-fusion, fission, movement, and mitophagy-in neurodegenerative diseases. Hum. Mol. Genet. 18, R169-R176 (2009).
24. Archer, S. L. Mitochondrial dynamics-mitochondrial fission and fusion in human diseases. N. Engl. J. Med. 369, 2236-2251 (2013).
25. Braun, R. J., Westermann, B. Mitochondrial dynamics in yeast cell death and aging. Biochem. Soc. Trans. 39, 1520-1526 (2011).
26. Scheckhuber, C. Q. et al. Reducing mitochondrial fission results in increased life span and fitness of two fungal ageing models. Nat. Chem. Biol. 9, 99-105 (2007).
27. Scheckhuber, C. Q.,Wanger, R. A., Mignat, C. A., Osiewacz, H. D. Unopposed mitochondrial fission leads to severe lifespan shortening. Cell Cycle 10, 3105-3110 (2011).
28. Deutsch, J. et al. Mitochondrial genetics. VI. The petite mutation in Saccharomyces cerevisiae: interrelations between the loss of the p1factor and the loss of the drug resistance mitochondrial genetic markers. Genetics 76, 195-219 (1974).
29. Osiewacz, H. D., Bernhardt, D. Mitochondrial quality control: impact on aging and life span-a mini-review. Gerontology 59, 413-420 (2013).
30. Fischer, F., Hamann, A., Osiewacz, H. D. Mitochondrial quality control: An integrated network of pathways. Trends Biochem. Sci. 37, 284-292 (2012).
31. Mendl, N. et al. Mitophagy in yeast is independent of mitochondrial fission and requires the stress response gene WHI2. J. Cell Sci. 124, 1339-1350 (2011).
32. Lewis, M. R., Lewis, W. H. Mitochondria in tissue culture. Science 39, 330-333 (1914).
33. Zuchner, S. et al. Mutations in the mitochondrial GTPase mitofusin 2 cause Charcot-Marie-Tooth neuropathy type 2A. Nat. Genet. 36, 449-451 (2004).
34. Olichon, A. et al. Mitochondrial dynamics and disease, OPA1. Biochim. Biophys. Acta 1763, 500-509 (2006).
35. Waterham, H. R. et al. A lethal defect of mitochondrial and peroxisomal fission. N. Engl. J. Med. 356, 1736-1741 (2007).
36. Figge, M. T., Reichert, A. S., Meyer-Hermann,M., Osiewacz, H. D. Deceleration of fusion-fission cycles improves mitochondrial quality control during aging. PLoS Comput. Biol. 8, e1002576 (2012).
37. Detmer, S. A., Chan, D. C. Functions and dysfunctions of mitochondrial dynamics. Nat. Rev. Mol. Cell Biol. 8, 870-879 (2007).
38. Figge, M. T., Osiewacz, H. D., Reichert, A. S. Quality control of mitochondria during aging: Is there a good and a bad side of mitochondrial dynamics Bioessays 35, 314-322 (2013).
39. Harju, S., Fedosyuk, H., Peterson, K. R. Rapid isolation of yeast genomic DNA: Bust n' Grab. BMC. Biotechnol. 4, 8 (2004).
40. Westermann, B., Neupert, W. Mitochondria-targeted green fluorescent proteins: convenient tools for the study of organelle biogenesis in Saccharomyces cerevisiae. Yeast 16, 1421-1427 (2000).
41. Meisinger, C., Pfanner, N., Truscott, K. N. Isolation of yeast mitochondria. Methods Mol. Biol. 313, 33-39 (2006).