Towards detection and diagnosis of Ebola virus disease at point-of-care

Biosensors and Bioelectronics

Volumn 75, Issue , 2016, Pages 254-272

  Kaushik, Ajeet ^a   Tiwari, Sneham ^a   Dev Jayant, Rahul ^a   Marty, Aileen ^b   Nair, Madhavan ^a  

^a Institute of NeuroImmune Pharmacology   (United States)

^b FLORIDA INTERNATIONAL UNIVERSITY   (United States)

Author keywords

Ebola diagnostics; Ebola sensor; Ebola therapeutics; Ebola Virus diseases; Point of care sensing

Indexed keywords

ANTIBODIES; ANTIGENS; CHEMICAL DETECTION; COST EFFECTIVENESS; DIAGNOSIS; VIRUSES;

ANTIBODY RESPONSE; DETECTION AND DIAGNOSIS; EBOLA THERAPEUTICS; EBOLA VIRUS; HEALTH EMERGENCIES; PM CONCENTRATIONS; POINT OF CARE; WORLD HEALTH ORGANIZATION;

DISEASES;

ADENOVIRUS VACCINE; EBOLA VACCINE; FAVIPIRAVIR; PROTEIN VP40; ZMAPP;

ARTICLE; BIOSENSOR; BLOOD ANTIBODY ANALYSIS; CD4+ T LYMPHOCYTE; CD8+ T LYMPHOCYTE; DIAGNOSTIC KIT; DIAGNOSTIC TEST; DRUG SAFETY; EBOLA HEMORRHAGIC FEVER; ELECTROCHEMICAL IMMUNOSENSING; ELECTROCHEMICAL IMMUNOSENSOR; ELECTRON MICROSCOPY; ENZYME LINKED IMMUNOSORBENT ASSAY; FLUORESCENCE MICROSCOPY; GENETIC PROCEDURES; GENETIC VARIABILITY; HEALTH CARE DELIVERY; HUMAN; IMMUNIZATION; IMMUNOFLUORESCENCE TEST; IMMUNOGENICITY; IMMUNOHISTOCHEMISTRY; IMMUNOSENSOR; INDIRECT IMMUNOFLUORESCENCE ASSAY; INFECTION CONTROL; INFECTION PREVENTION; INTERMETHOD COMPARISON; LABORATORY DIAGNOSIS; LIFE CYCLE; LIMIT OF DETECTION; NONHUMAN; POINT OF CARE; PROTEIN STRUCTURE; QUARTZ CRYSTAL MICROBALANCE; REAL TIME POLYMERASE CHAIN REACTION; SURFACE PLASMON RESONANCE; SYMPTOMATOLOGY; VIRAL DISEASE IMMUNOFLUORESCENCE ASSAY; VIRUS DETECTION; VIRUS GENOME; VIRUS PARTICLE; VIRUS REPLICATION; VIRUS TRANSMISSION; WESTERN BLOTTING; ZAIRE EBOLAVIRUS; EBOLAVIRUS; EPIDEMIC; HEMORRHAGIC FEVER, EBOLA; ISOLATION AND PURIFICATION; PATHOGENICITY; POINT OF CARE SYSTEM; VIROLOGY;

ANIMALIA; EBOLA VIRUS;

BIOSENSING TECHNIQUES; DISEASE OUTBREAKS; EBOLAVIRUS; HEMORRHAGIC FEVER, EBOLA; HUMANS; POINT-OF-CARE SYSTEMS;

EID: 84940094098     PISSN: 09565663     EISSN: 18734235     Source Type: Journal    
DOI: 10.1016/j.bios.2015.08.040     Document Type: Review

References (94)

1. Baden L.R., Kanapathipillai R., Campion E.W., Morrissey S., Rubin E.J., Drazen J.M. Ebola - an ongoing crisis. N. Engl. J. Med. 2014, 371(15):1458-1459.
2. Bah E.I., Lamah M.-C., Fletcher T., Jacob S.T., Brett-Major D.M., Sall A.A., Shindo N., Fischer W.A., Lamontagne F., Saliou S.M., Bausch D.G., Moumiã B., Jagatic T., Sprecher A., Lawler J.V., Mayet T., Jacquerioz F.A., Mendez Baggi M.F., Vallenas C., Clement C., Mardel S., Faye O., Faye O., Soropogui B., Magassouba N., Koivogui L., Pinto R., Fowler R.A. Clinical Presentation of Patients with Ebola Virus Disease in Conakry, Guinea. N. Engl. J. Med. 2014, 372:40-47.
3. Baize S., Leroy E., Georges A., GEORGESâ COURBOT M.C., Capron M., Bedjabaga I., LANSOUDâ SOUKATE J., Mavoungou E. Inflammatory responses in Ebola virusâ infected patients. Clin. Exp. Immunol. 2002, 128(1):163-168.
4. Baize S., Pannetier D., Oestereich L., Rieger T., Koivogui L., Magassouba N.F., Soropogui B., Sow M.S., KeÃ-ta S., De Clerck H., Tiffany A., Dominguez G., Loua M., Traorã A., Koliã M., Malano E.R., Heleze E., Bocquin A., Mãly S., Raoul H., Caro Vr, Cadar Dn, Gabriel M., Pahlmann M., Tappe D., Schmidt-Chanasit J., Impouma B., Diallo A.K., Formenty P., Van Herp M., Gã1/4nther S. Emergence of Zaire Ebola Virus Disease in Guinea. N. Engl. J. Med. 2014, 371(15):1418-1425.
5. Bar S., Takada A., Kawaoka Y., Alizon M. Detection of cell-cell fusion mediated by Ebola virus glycoproteins. Journal of virology 2006, 80(6):2815-2822.
6. Beeching N.J., Fenech M., Houlihan C.F. Ebola virus disease. Br. Med. J. 2014, 349:g7348.
7. Bornholdt Z.A., Noda T., Abelson Dafna M., Halfmann P., Wood Malcolm R., Kawaoka Y., Saphire Erica O. Structural Rearrangement of Ebola Virus VP40 Begets Multiple Functions in the Virus Life Cycle. Cell 2013, 154(4):763-774.
8. Breman J.G., Johnson K.M. Ebola Then and Now. N. Engl. J. Med. 2014, 371(18):1663-1666.
9. Briand S., Bertherat E., Cox P., Formenty P., Kieny M.-P., Myhre J.K., Roth C., Shindo N., Dye C. The International Ebola Emergency. N. Engl. J. Med. 2014, 371(13):1180-1183.
10. Burd E.M. Ebola Virus: a Clear and Present Danger. J. Clin. Microbiol. 2015, 53(1):4-8.
11. Butler D. Ebola experts seek to expand testing. Nature 2014, 516.
12. Carette J.E., Raaben M., Wong A.C., Herbert A.S., Obernosterer G., Mulherkar N., Kuehne A.I., Kranzusch P.J., Griffin A.M., Ruthel G. Ebola virus entry requires the cholesterol transporter Niemann-Pick C1. Nature 2011, 477(7364):340-343.
13. Carod-Artal F.J. Illness due the Ebola virus: epidemiology and clinical manifestations within the context of an international public health emergency. Rev. Neurol. 2015, 60(6):267-277.
14. Chan M. Ebola Virus Disease in West Africa â" No Early End to the Outbreak. N. Engl. J. Med. 2014, 371(13):1183-1185.
15. Chandran K., Sullivan N.J., Felbor U., Whelan S.P., Cunningham J.M. Endosomal proteolysis of the Ebola virus glycoprotein is necessary for infection. Science 2005, 308(5728):1643-1645.
16. Chertow D.S., Kleine C., Edwards J.K., Scaini R., Giuliani R., Sprecher A. Ebola Virus Disease in West Africa â" Clinical Manifestations and Management. N. Engl. J. Med. 2014, 371(22):2054-2057.
17. Choi J.H., Croyle M.A. Emerging targets and novel approaches to Ebola virus prophylaxis and treatment. BioDrugs 2013, 27(6):565-583.
18. Choi J.H., Jonsson-Schmunk K., Qiu X., Shedlock D.J., Strong J., Xu J.X., Michie K.L., Audet J., Fernando L., Myers M.J., Weiner D., Bajrovic I., Tran L.Q., Wong G., Bello A., Kobinger G.P., Schafer S.C., Croyle M.A. A single dose respiratory recombinant adenovirus-based vaccine provides long-term protection for non-human primates from lethal Ebola infection. Mol. Pharmaceut. 2014.
19. Cowling B.J., Yu H. Ebola: worldwide dissemination risk and response priorities. The Lancet 2015, 385(9962):7-9.
20. Cruz A.F.D., Norena N., Kaushik A., Bhansali S. A low-cost miniaturized potentiostat for point-of-care diagnosis. Biosens. Bioelectron. 2014, 62:249-254.
21. Daaboul G.G., Lopez C.A., Chinnala J., Goldberg B.B., Connor J.H., Unlu M.S. Digital sensing and sizing of vesicular stomatitis virus pseudotypes in complex media: a model for ebola and Marburg detection. ACS nano 2014, 8(6):6047-6055.
22. De Clercq E. Ebola virus (EBOV) infection: Therapeutic strategies. Biochem. Pharmacol. 2015, 93(1):1-10.
23. Dhillon R.S., Srikrishna D., Sachs J. Controlling Ebola: next steps. The Lancet 2014, 384(9952):1409-1411.
24. Drazen J.M., Campion E.W., M.D.E.J.R., Morrissey S., Baden L.R. Ebola in West Africa at One Year - From Ignorance to Fear to Roadblocks. N. Engl. J. Med. 2014, 0(0). null.
25. Drosten C., Gãttig S., Schilling S., Asper M., Panning M., Schmitz H., Gã1/4nther S. Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription-PCR. J. Clin. Microbiol. 2002, 40(7):2323-2330.
26. Farrar J.J., Piot P. The Ebola emergency - immediate action, ongoing strategy. N. Engl. J. Med. 2014, 371(16):1545-1546.
27. Fauci A.S. Ebola-underscoring the global disparities in health care resources. N. Engl. J. Med. 2014, 371(12):1084-1086.
28. Feldmann H. Ebola-A growing threat?. N. Engl. J. Med. 2014, 371(15):1375-1378.
29. Feldmann H. Ebola -A Growing Threat?. N. Engl. J. Med. 2014, 371(15):1375-1378.
30. Feldmann H., Geisbert T.W. Ebola hemorrhagic fever. The Lancet 2011, 377(9768):849-862.
31. Feldmann H., Wahl-Jensen V., Jones S.M., Strãher U. Ebola virus ecology: a continuing mystery. Trends Microbiol. 2004, 12(10):433-437.
32. Formenty P., Leroy E.M., Epelboin A., Libama Fo, Lenzi M., Sudeck H., Yaba P., Allarangar Y., Boumandouki P., Nkounkou V.B. Detection of Ebola virus in oral fluid specimens during outbreaks of Ebola virus hemorrhagic fever in the Republic of Congo. Clin. Infect. Dis. 2006, 42(11):1521-1526.
33. Frieden T.R., Damon I., Bell B.P., Kenyon T., Nichol S. Ebola 2014 - New Challenges, New Global Response and Responsibility. N. Engl. J. Med. 2014, 371(13):1177-1180.
34. Gire S.K., Goba A., Andersen K.G., Sealfon R.S., Park D.J., Kanneh L., Jalloh S., Momoh M., Fullah M., Dudas G. Genomic surveillance elucidates Ebola virus origin and transmission during the 2014 outbreak. Science 2014, 345(6202):1369-1372.
35. Goodman J.L. Studying Secret Serums- Toward Safe, Effective Ebola Treatments. N. Engl. J. Med. 2014, 371(12):1086-1089.
36. Grolla A., Lucht A., Dick D., Strong J., Feldmann H. Laboratory diagnosis of Ebola and Marburg hemorrhagic fever. Bull. Soc. Pathol. Exotique 2005, 98(3):205.
37. Hatfill S.J., Nordin T., Shapiro G.L. Ebola Virus Disease. J. Am. Phys. Surgeons 2014, 19:4.
38. Hill C.E., Burd E.M., Kraft C.S., Ryan E.L., Duncan A., Winkler A.M., Cardella J.C., Ritchie J.C., Parslow T.G. Laboratory test support for Ebola patients within a high-containment facility. Lab Med. 2014, 45(3):e109-e111.
39. Huang Y., Wei H., Wang Y., Shi Z., Raoul H., Yuan Z. Rapid detection of filoviruses by real-time TaqMan polymerase chain reaction assays. Virol. Sin. 2012, 27(5):273-277.
40. Ikegami T., Saijo M., Niikura M., Miranda M.E., Calaor A.B., Hernandez M., Manalo D.L., Kurane I., Yoshikawa Y., Morikawa S. Development of an immunofluorescence method for the detection of antibodies to ebola virus subtype reston by the use of recombinant nucleoproteinâ expressing HeLa Cells. Microbiol. Immunol. 2002, 46(9):633-638.
41. Iwen P.C., Garrett J.L., Gibbs S.G., Lowe J.J., Herrera V.L., Sambol A.R., Stiles K., Wisecarver J.L., Salerno K.J., Pirruccello S.J. An integrated approach to laboratory testing for patients with ebola virus disease. Lab Med. 2014, 45(4):e146-e151.
42. Johnson J.C., Martinez O., Honko A.N., Hensley L.E., Olinger G.G., Basler C.F. Pyridinyl imidazole inhibitors of p38 MAP kinase impair viral entry and reduce cytokine induction by Zaire ebolavirus in human dendritic cells 2014, 102-109.
43. Kanapathipillai R. Ebola Virus Disease: Current Knowledge. N. Engl. J. Med. 2014, 371(13):e18.
44. Kanapathipillai R., Henao Restrepo A.M., Fast P., Wood D., Dye C., Kieny M.-P., Moorthy V. Ebola Vaccine- An Urgent International Priority. N. Engl. J. Med. 2014, 371(24):2249-2251.
45. Karwowska K. Ebola virus disease. Polski merkuriusz lekarski: organ Polskiego Towarzystwa Lekarskiego 2015, 38(223):42-45.
46. Kaushik A., Ruiz A., Bhansali S., Nair M. Miniaturized Sensing Devices for Biomarker Detection. J. Biosens. Bioelectron. 2014, 5(4):1000e1132.
47. Kaushik A., Vasudev A., Arya S.K., Pasha S.K., Bhansali S. Recent advances in cortisol sensing technologies for point-of-care application. Biosens. Bioelectron. 2014, 53:499-512.
48. Kawaoka Y. How Ebola Virus Infects Cells. N. Engl. J. Med. 2005, 352(25):2645-2646.
49. Kurosaki Y., Takada A., Ebihara H., Grolla A., Kamo N., Feldmann H., Kawaoka Y., Yasuda J. Rapid and simple detection of Ebola virus by reverse transcription-loop-mediated isothermal amplification. J. Virol. Methods 2007, 141(1):78-83.
50. Lamontagne Fo, Clement C., Fletcher T., Jacob S.T., Fischer W.A., Fowler R.A. Doing Today Work Superbly Well â" Treating Ebola with Current Tools. N. Engl. J. Med. 2014, 371(17):1565-1566.
51. Li H., Ying T., Yu F., Lu L., Jiang S. Development of therapeutics for treatment of Ebola virus infection. Microbes and Infection 2014.
52. Li Y., Cu Y.T.H., Luo D. Multiplexed detection of pathogen DNA with DNA-based fluorescence nanobarcodes. Nature biotechnology 2005, 23(7):885-889.
53. Lucht A., Formenty P., Feldmann H., Gãtz M., Leroy E., Bataboukila P., Grolla A., Feldmann F., Wittmann T., Campbell P. Development of an immunofiltration-based antigen-detection assay for rapid diagnosis of Ebola virus infection. J. Infect. Dis. 2007, 196(Suppl. 2):S184-S192.
54. Lucht A., Grunow R., Mãller P., Feldmann H., Becker S. Development, characterization and use of monoclonal VP40-antibodies for the detection of Ebola virus. J. Virol. Methods 2003, 111(1):21-28.
55. Lyon G.M., Mehta A.K., Varkey J.B., Brantly K., Plyler L., McElroy A.K., Kraft C.S., Towner J.S., Spiropoulou C., Strãher U., Uyeki T.M., Ribner B.S. Clinical care of two patients with Ebola virus disease in the United States. N. Engl. J. Med. 2014, 371(25):2402-2409.
56. MacIntyre C.R., Chughtai A.A., Seale H., Richards G.A., Davidson P.M. Respiratory protection for healthcare workers treating Ebola virus disease (EVD): Are facemasks sufficient to meet occupational health and safety obligations?. Int. J. Nurs. Stud. 2014, 51:1421-1426.
57. Maganga Gl.D., Kapetshi J., Berthet N., Kebela Ilunga Bt, Kabange F., Mbala Kingebeni P., Mondonge V., Muyembe J.-J.T., Bertherat E., Briand S., Cabore J., Epelboin A., Formenty P., Kobinger G., GonzÃlez-Angulo L., Labouba I., Manuguerra J.-C., Okwo-Bele J.-M., Dye C., Leroy E.M. Ebola Virus Disease in the Democratic Republic of Congo. N. Engl. J. Med. 2014, 371(22):2083-2091.
58. Martin P., Laupland K.B., Frost E.H., Valiquette L. Laboratory diagnosis of Ebola virus disease. Intens. Care Med. 2015, 1-4.
59. Martines R.B., Ng D.L., Greer P.W., Rollin P.E., Zaki S.R. Tissue and cellular tropism, pathology and pathogenesis of Ebola and Marburg viruses. J. Pathol. 2015, 235(2):153-174.
60. Marzi A., Engelmann F., Feldmann F., Haberthur K., Shupert W.L., Brining D., Scott D.P., Geisbert T.W., Kawaoka Y., Katze M.G. Antibodies are necessary for rVSV/ZEBOV-GPâ"mediated protection against lethal Ebola virus challenge in nonhuman primates. Proc. Natl. Acad. Sci. 2014, 110(5):1893-1898.
61. Miller E.H., Obernosterer G., Raaben M., Herbert A.S., Deffieu M.S., Krishnan A., Ndungo E., Sandesara R.G., Carette J.E., Kuehne A.I. Ebola virus entry requires the hostâ programmed recognition of an intracellular receptor. EMBO J. 2012, 31(8):1947-1960.
62. Mitman G. Ebola in a Stew of Fear. N. Engl. J. Med. 2014, 371(19):1763-1765.
63. Mohan G.S., Li W., Ye L., Compans R.W., Yang C. Antigenic subversion: a novel mechanism of host immune evasion by Ebola virus. PLoS Pathogens 2012, 8(12):e1003065.
64. Nakayama E., Yokoyama A., Miyamoto H., Igarashi M., Kishida N., Matsuno K., Marzi A., Feldmann H., Ito K., Saijo M. Enzyme-linked immunosorbent assay for detection of filovirus species-specific antibodies. Clin. Vaccine Immunol. 2010, 17(11):1723-1728.
65. Niikura M., Ikegami T., Saijo M., Kurane I., Miranda M.E., Morikawa S. Detection of Ebola viral antigen by enzyme-linked immunosorbent assay using a novel monoclonal antibody to nucleoprotein. J. Clin. Microbiol. 2001, 39(9):3267-3271.
66. Nyakatura E.K., Frei J.C., Lai J.R. Chemical and structural aspects of Ebola virus entry inhibitors. ACS Infect. Dis. 2015, 1(1):42-52.
67. Pardee K., Green A.A., Ferrante T., Cameron D.E., DaleyKeyser A., Yin P., Collins J.J. Paper-based synthetic gene networks. Cell 2014, 159(4):940-954.
68. Peters C., Peters J. An introduction to Ebola: the virus and the disease. J. Infect. Dis. 1999, 179(Suppl. 1). ix-xvi.
69. Prehaud C., Hellebrand E., Coudrier D., Volchkov V., Volchkova V., Feldmann H., Le Guenno B., Bouloy M. Recombinant Ebola virus nucleoprotein and glycoprotein (Gabon 94 strain) provide new tools for the detection of human infections. J. Gen. Virol. 1998, 79(11):2565-2572.
70. Rivera A., Messaoudi I. Pathophysiology of Ebola virus infection: Current challenges and future hopes. ACS Infect. Dis. 2015.
71. Rosenbaum L. Communicating Uncertainty - Ebola, Public Health, and the Scientific Process. N. Engl. J. Med. 2015, 372(1):7-9.
72. Rosenbaum L. License to Serve - U.S. Trainees and the Ebola Epidemic. N. Engl. J. Med. 2015, 372(6):504-506.
73. Rougeron, V., Feldmann, H., Grard, G., Becker, S., Leroy, E.M., 2015. Ebola and Marburg hemorrhagic fever.
74. Sagripanti J.-L., Lytle C.D. Sensitivity to ultraviolet radiation of Lassa, vaccinia, and Ebola viruses dried on surfaces. Arch. Virol. 2011, 156(3):489-494.
75. Saijo M., Niikura M., Ikegami T., Kurane I., Kurata T., Morikawa S. Laboratory diagnostic systems for Ebola and Marburg hemorrhagic fevers developed with recombinant proteins. Clin. Vaccine Immunol. 2006, 13(4):444-451.
76. Saijo M., Niikura M., Morikawa S., Kurane I. Immunofluorescence method for detection of Ebola virus immunoglobulin G, using HeLa cells which express recombinant nucleoprotein. J. Clin. Microbiol. 2001, 39(2):776-778.
77. Sanchez A., Ksiazek T.G., Rollin P.E., Miranda M.E., Trappier S.G., Khan A.S., Peters C.J., Nichol S.T. Detection and molecular characterization of Ebola viruses causing disease in human and nonhuman primates. J. Infect. Dis. 1999, 179(Suppl. 1):S164-S169.
78. Stanley D.A., Honko A.N., Asiedu C., Trefry J.C., Lau-Kilby A.W., Johnson J.C., Hensley L., Ammendola V., Abbate A., Grazioli F., Foulds K.E., Cheng C., Wang L., Donaldson M.M., Colloca S., Folgori A., Roederer M., Nabel G.J., Mascola J., Nicosia A., Cortese R., Koup R.A., Sullivan N.J. Chimpanzee adenovirus vaccine generates acute and durable protective immunity against ebolavirus challenge 2014, Nat Med advance online publication.
79. Sullivan N., Yang Z.-Y., Nabel G.J. Ebola virus pathogenesis: implications for vaccines and therapies. J. Virol. 2003, 77(18):9733-9737.
80. Sullivan N.J., Geisbert T.W., Geisbert J.B., Xu L., Yang Z.-y, Roederer M., Koup R.A., Jahrling P.B., Nabel G.J. Accelerated vaccination for Ebola virus hemorrhagic fever in non-human primates. Nature 2003, 424(6949):681-684.
81. Sullivan N.J., Sanchez A., Rollin P.E., Yang Z.-y, Nabel G.J. Development of a preventive vaccine for Ebola virus infection in primates. Nature 2000, 408(6812):605-609.
82. Swamy M.A., Nair M.P., Saxena S.K. Current scenario of therapeutics for Ebola virus disease. Am. J. Infect. Dis. 2014, 10(3):100.
83. Takada A. Filovirus tropism: cellular molecules for viral entry. Front. Microbiol. 2012, 3:34.
84. Tan D.X., Korkmaz A., Reiter R.J., Manchester L.C. Ebola virus disease: Potential use of melatonin as a treatment. J. Pin. Res. 2014, 57(4):381-384.
85. Trombley A.R., Wachter L., Garrison J., Buckley-Beason V.A., Jahrling J., Hensley L.E., Schoepp R.J., Norwood D.A., Goba A., Fair J.N. Comprehensive Panel of Real-Time TaqManâ"¢ Polymerase Chain Reaction Assays for Detection and Absolute Quantification of Filoviruses, Arenaviruses, and New World Hantaviruses. Am. J. Trop. Med. Hyg. 2010, 82(5):954-960.
86. Uebelhoer, L.S., Albarião, Cs.G., McMullan, L.K., Chakrabarti, A.K., Vincent, J.P., Nichol, S.T., Towner, J.S., 2014. High-throughput luciferase-based reverse genetics systems for identifying inhibitors of Marburg and Ebola viruses. 86-94.
87. Van Kinh Nguyen S.C.B., Boianelli A., Meyer-Hermann M., Hernandez-Vargas E.A. Ebola virus infection modeling and identifiability problems. Front. Microbiol. 2015, 6.
88. Vasudev A., Kaushik A., Tomizawa Y., Norena N., Bhansali S. An LTCC-based microfluidic system for label-free, electrochemical detection of cortisol. Sens. Actuators B: Chem. 2013, 182:139-146.
89. Vogel G. Testing new Ebola tests. Science 2014, 345(6204):1549-1550.
90. Wang Y., Liu Z., Dai Q. A highly immunogenic fragment derived from Zaire Ebola virus glycoprotein elicits effective neutralizing antibody 2014, 254-261.
91. Xu L., Sanchez A., Yang Z.-Y., Zaki S.R., Nabel E.G., Nichol S.T., Nabel G.J. Immunization for Ebola virus infection. Nat. Med. 1998, 4(1):37-42.
92. Yang Z.-y, Delgado R., Xu L., Todd R.F., Nabel E.G., Sanchez A., Nabel G.J. Distinct cellular interactions of secreted and transmembrane Ebola virus glycoproteins. Science 1998, 279(5353):1034-1037.
93. Yanik A.A., Huang M., Kamohara O., Artar A., Geisbert T.W., Connor J.H., Altug H. An optofluidic nanoplasmonic biosensor for direct detection of live viruses from biological media. Nano Lett. 2010, 10(12):4962-4969.
94. Yu J.-S., Liao H.-X., Gerdon A.E., Huffman B., Scearce R.M., McAdams M., Alam S.M., Popernack P.M., Sullivan N.J., Wright D. Detection of Ebola virus envelope using monoclonal and polyclonal antibodies in ELISA, surface plasmon resonance and a quartz crystal microbalance immunosensor. J. Virol. Methods 2006, 137(2):219-228.