Development of an immunofluorescence method for the detection of antibodies to Ebola virus subtype reston by the use of recombinant nucleoprotein-expressing HeLa cells

Microbiology and Immunology

Volumn 46, Issue 9, 2002, Pages 633-638

  Ikegami, Tetsuro ^a   Saijo, Masayuki ^a   Niikura, Masahiro ^a   Miranda, Mary E G ^a   Calaor, Alan B ^a   Hernandez, Marvin ^a   Manalo, Daria L ^a   Kurane, Ichiro ^a   Yoshikawa, Yasuhiro ^a   Morikawa, Shigeru ^a  

^a NATIONAL INSTITUTE OF INFECTIOUS DISEASES   (Japan)

Author keywords

Cynomolgus macaque; Ebola virus subtype Reston; IFA; Recombinant nucleoprotein

Indexed keywords

NUCLEOPROTEIN; RECOMBINANT PROTEIN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBODY DETECTION; ANTIBODY SPECIFICITY; ARTICLE; CONTROLLED STUDY; EBOLA VIRUS; EBOLA VIRUS SUBTYPE RESTON; EBOLA VIRUS SUBTYPE ZAIRE; EPIDEMIC; HELA CELL; IMMUNOFLUORESCENCE TEST; MONKEY; NONHUMAN; NUCLEOTIDE SEQUENCE; PHILIPPINES; PROTEIN EXPRESSION; SENSITIVITY AND SPECIFICITY; SEROEPIDEMIOLOGY; VIRUS CLASSIFICATION; VIRUS INFECTION;

ANIMALS; ANTIBODIES, VIRAL; EBOLAVIRUS; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; HELA CELLS; HEMORRHAGIC FEVER, EBOLA; HUMANS; MACACA FASCICULARIS; MONKEY DISEASES; NUCLEOPROTEINS; PLASMIDS; RABBITS; RECOMBINANT PROTEINS; SENSITIVITY AND SPECIFICITY; TRANSFECTION;

ANIMALIA; EBOLA VIRUS; ORYCTOLAGUS CUNICULUS; RESTON EBOLAVIRUS; RNA VIRUSES; ZAIRE EBOLAVIRUS;

EID: 0036379078     PISSN: 03855600     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1348-0421.2002.tb02745.x     Document Type: Article

References (25)

1. Bouree, P., and Bergmann, J.F. 1983. Ebola virus infection in man: a serological and epidemiological survey in the Cameroons. Am. J. Trop. Med. Hyg. 32: 1465-1456.
2. Centers for Disease Control and Prevention. 1996. Ebola-Reston virus infection among quarantined nonhuman primates-Texas, 1996. MMWR 45: 314-316.
3. Centers for Disease Control and Prevention. 1990. Epidemiologic notes and reports updates: filovirus infection in animal handlers. MMWR 39: 221.
4. Centers for Disease Control and Prevention. 1990. Update: filovirus infections among persons with occupational exposure to nonhuman primates. MMWR 39: 266-273.
5. Elliott, L.H., Bauer, S.P., Perez-Oronoz, G., and Lloyd, E.S. 1993. Improved specificity of testing methods for filovirus antibodies. J. Virol. Methods 43: 85-99.
6. Feldmann, H., and Kiley, M.P. 1999. Classification, structure, and replication of filoviruses. Curr. Top. Microbiol. Immunol. 235: 1-21.
7. Feldmann, H., Klenk, H.D., and Sanchez, A. 1993. Molecular biology and evolution of filoviruses. Arch. Virol. (Suppl) 7: 81-100.
8. Hayes, C.G., Burans, J.P., Ksiazek, T.G., Del Rosario, R.A., Miranda, M.E., Manaloto, C.R., Barrientos, A.B., Robles, C.G., Dayrit, M.M., and Peters, C.J. 1992. Outbreak of fatal illness among captive macaques in the Philippines caused by an Ebola-related filovirus. Am. J. Trop. Med. Hyg. 46: 664-671.
9. Ikegami, T., Calaor, A.B., Miranda, M.E., Niikura, M., Saijo, M., Kurane, I., Yoshikawa, Y., and Morikawa, S. 2001. Genome structure of Ebola virus subtype Reston: differences among Ebola subtypes. Arch. Virol. 146: 2021-2027.
10. Jahrling, P.B., Geisbert, T.W., Dalgard, D.W., Johnson, E.D., Ksiazek, T.G., Hall, W.C., and Peters, C.J. 1990. Preliminary report: isolation of Ebola virus from monkeys imported to USA. Lancet 335: 502-505.
11. Johnson, K.M., Elliott, L.H., and Heymann, D.L. 1981. Preparation of polyvalent viral immunofluorescent intracellular antigens and use in human serosurvey. J. Clin. Microbiol. 14: 527-529.
12. Ksiazek, T.G., West, C.P., Rollin, P.E., Jahrling, P.B., and Peters, C.J. 1999. ELISA for the detection of antibodies to Ebola viruses. J. Infect. Dis. (Suppl) 179: 192-198.
13. Le Guenno, B., Formentry, P., Wyers, M., Gounon, P., Walker, F., and Boesch, C. 1995. Isolation and partial characterization of a new strain of Ebola virus. Lancet 345: 1271-1274.
14. Matsuura, Y., Possee, R.D., Overton, H.A., and Bishop, D.H. 1987. Baculovirus expression vectors: the requirements for high level expression of proteins, including glycoproteins. J. Gen. Virol. 68: 1233-1250.
15. Miranda, M.E., Ksiazek, T.G., Retuya, T.J., Khan, A.S., Sanchez, A., Fulhorst, C.F., Rollin, P.E., Calaor, A.B., Manalo, D.L., Roces, M.C., Dayrit, M.M., and Peters, C.J. 1999. Epidemiology of Ebola (subtype Reston) virus in the Philippines, 1996. J. Infect. Dis. (Suppl) 179: 115-119.
16. Mizushima, S., and Nagata, S. 1990. pEF-BOS, a powerful mammalian expression vector. Nucleic Acids Res. 18: 5322.
17. Rollin, P.E., Williams, R.J., Bressler, D.S., Pearson, S., Cottingham, M., Pucak, G., Sanchez, A., Trappier, S.G., Peters, R.L., Greer, P.W., Zaki, S., Demarcus, T., Hendricks, K., Kelley, M., Simpson, D., Geisbert, T.W., Jahrling, P.B., Peters, C.J., and Ksiazek, T.G. 1999. Ebola (subtype Reston) virus among quarantined nonhuman primates recently imported from the Philippines to United States. J. Infect. Dis. (Suppl) 179: 108-114.
18. Saijo, M., Niikura, M., Morikawa, S., and Kurane, I. 2001. Immunofluorescence method for detection of Ebola virus immunoglobulin G, using HeLa cells which express recombinant nucleoprotein. J. Clin. Microbiol. 39: 776-778.
19. Saijo, M., Niikura, M., Morikawa, S., Ksiazek, T.G., Meyer, R.F., Peters, C.J., and Kurane, I. 2001. Enzyme-linked immunosorbent assays for detection of antibodies to Ebola and Marburg viruses using recombinant nucleoproteins J. Clin. Microbiol. 39: 1-7.
20. Saijo, M., Qing, T., Niikura, M., Maeda, A., Ikegami, T., Sakai, W., Prehaud, C., Kurane, I., and Morikawa, S. 2002. Immunofluorescence technique using HeLa cells expressing recombinant nucleoprotein for detection of immunoglobulin G antibodies to Crimean-Congo hemorrhagic fever virus. J. Clin. Microbiol. 40: 372-375.
21. Van der Groen, G., Kurata, T., and Mets, C. 1983. Modifications to indirect immunofluorescence tests on Lassa, Marburg, and Ebola material. Lancet 1: 654-655.
22. Van der Waals, F.W., Pomeroy, K.L., Goudsmit, J., Asher, D.M., and Gajdusek, D.C. 1986. Hemorrhagic fever virus infections in an isolated rainforest area of central Liberia. Limitations of the indirect immunofluorescence slide test for antibody screening in Africa. Trop. Geogr. Med. 38: 209-214.
23. World Health Organization. 1976. Ebola haemorrhagic fever in Sudan, 1976. Bull. World Health Organ. 56: 247-270.
24. World Health Organization. 1978. Ebola haemorrhagic fever in Zaire, 1976. Report of an international commission. Bull. World Health Organ. 56: 271-293.
25. World Health Organization. 1992. Viral haemorrhagic fever in imported monkeys. Wkly. Epidemiol. Rec. 67: 142-143.