A one-step preparation method of monolithic enzyme reactor for highly efficient sample preparation coupled to mass spectrometry-based proteomics studies

Journal of Chromatography A

Volumn 1412, Issue , 2015, Pages 75-81

  Jiang, Shan ^a   Zhang, Zichuan ^a   Li, Lingjun ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Database search; Digestion; ESI orbitrap MS; IMER; MALDI MS; Monolithic column; Protein identification; Proteomics

Indexed keywords

AMIDES; BIOREACTORS; DRUG PRODUCTS; ENZYMES; FUSED SILICA; MAMMALS; MASS SPECTROMETRY; SEARCH ENGINES; SLUDGE DIGESTION;

DATABASE SEARCHES; IMER; MALDI-MS; MONOLITHIC COLUMNS; ORBITRAP MS; PROTEIN IDENTIFICATION;

PROTEOMICS;

BOVINE SERUM ALBUMIN; CHEMICAL COMPOUND; LYSINE; N ACRYLOXYSUCCINIMIDE CO ACRYLAMIDE CO N,N' METHYLENEBISACRYLAMIDE; TRYPSIN; UNCLASSIFIED DRUG; ACRYLIC ACID RESIN; IMMOBILIZED ENZYME; PROTEIN; SILICON DIOXIDE;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CELL LYSATE; CONJUGATION; CONTROLLED STUDY; HUMAN; IMMOBILIZED ENZYME REACTOR; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN DEGRADATION; PROTEIN DETERMINATION; PROTEOMICS; YEAST CELL; ANIMAL; BIOREACTOR; BOVINE; CHEMISTRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MOUSE; PROCEDURES; SERUM; YEAST;

BOVINAE;

ACRYLIC RESINS; ANIMALS; BIOREACTORS; CATTLE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ENZYMES, IMMOBILIZED; HUMANS; MICE; PROTEINS; PROTEOMICS; SERUM; SERUM ALBUMIN, BOVINE; SILICON DIOXIDE; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TRYPSIN; YEASTS;

EID: 84940036458     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2015.07.121     Document Type: Article

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