Feedback-resistant acetohydroxy acid synthase increases valine production in Corynebacterium glutamicum

Applied and Environmental Microbiology

Volumn 71, Issue 1, 2005, Pages 207-213

  Elišáková, Veronika ^a   Pátek, Miroslav ^a   Holátko, Jiří ^a   Ňesvera, Jan ^a   Leyval, Damien ^b   Goergen, Jean Louis ^c   Delaunay, Stéphane ^b  

^a INSTITUTE OF MICROBIOLOGY   (Czech Republic)

^b UNIVERSITÉ DE LORRAINE   (France)

^c CNRS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BIOSYNTHESIS; CHROMOSOMES; ENZYMES; MUTAGENESIS; VECTORS;

ACETOHYDROXY ACID SYNTHASE (AHAS); ISOLEUCINE; LEUCINE; VALINE;

BACTERIA;

ACETOLACTATE SYNTHASE; ISOLEUCINE; KETOL ACID REDUCTOISOMERASE; LEUCINE; OXIDOREDUCTASE; UNCLASSIFIED DRUG; VALINE;

ENZYME;

AMINO ACID SYNTHESIS; ARTICLE; BACTERIAL METABOLISM; BACTERIAL STRAIN; BINDING SITE; BIOSYNTHESIS; CATALYSIS; CORYNEBACTERIUM GLUTAMICUM; FEEDBACK SYSTEM; GENE MUTATION; NONHUMAN; OPERON; SITE DIRECTED MUTAGENESIS;

ACETOLACTATE SYNTHASE; AMINO ACID SEQUENCE; AMINO ACIDS, BRANCHED-CHAIN; BACTERIAL PROTEINS; CORYNEBACTERIUM GLUTAMICUM; GENE EXPRESSION REGULATION, BACTERIAL; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; OPERON; PLASMIDS; VALINE;

BACTERIA (MICROORGANISMS); CORYNEBACTERIUM; CORYNEBACTERIUM GLUTAMICUM; ESCHERICHIA COLI; POSIBACTERIA; UNIDENTIFIED SHUTTLE VECTOR;

EID: 12244275686     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.71.1.207-213.2005     Document Type: Article

References (30)

1. Eggeling, I., C. Cordes, L. Eggeling, and H. Sahm. 1987. Regulation of acetohydroxy acid synthase in Corynebacterium glutamicum during fermentation of alfa-ketobutyrate to L-isoleucine. Appl. Microbiol. Biotechnol. 25:346-351.
2. Epelbaum, S., R. A. LaRossa, T. K. VanDyk, T. Elkayam, D. M. Chipman, and Z. Barak. 1998. Branched-chain amino acid biosynthesis in Salmonella typhimurium: a quantitative analysis. J. Bacteriol. 180:4055-4067.
3. Hanahan, D. 1985. Techniques for transformation of E. coli, p. 109-135. In D. M. Glover (ed.), DNA cloning. A practical approach, vol. 1. IRL Press, Oxford, United Kingdom.
4. Horton, R. M. 1995. PCR-mediated recombination and mutagenesis. SOE-ing together tailor-made genes. Mol. Biotechnol. 3:93-99.
5. Inui, M., A. A. Vertes, M. Kobayashi, Y. Kurusu, and H. Yukawa. 1993. Cloning and sequence determination of the acetohydroxy acid synthase genes from Brevibacterium flavum MJ233 by using the polymerase chain reaction. DNA Sequence 3:303-310.
6. Ito, W., H. Ishiguro, and Y. Kurosawa. 1991. A general method for introducing a series of mutations into cloned DNA using the polymerase chain reaction. Gene 102:67-70.
7. Jäger, W., A. Schäfer, A. Fühler, G. Labes, and W. Wohlleben. 1992. Expression of the Bacillus subtilis sacB gene leads to sucrose sensitivity in the gram-positive bacterium Corynebacterium glutamicum but not in Streptomyces lividans. J. Bacteriol. 174:5462-5465.
8. Keilhauer, C., L. Eggeling, and H. Sahm. 1993. Isoleucine synthesis in Corynebacterium glutamicum: molecular analysis of the ilvB-ilvN-ilvC operon. J. Bacteriol. 175:5595-5603.
9. Kopecký, J., J. Janata, S. Pospíšil, J. Felsberg, and J. Spížek. 1999. Mutations in two distinct regions of acetolactate synthase regulatory subunit from Streptomyces cinnamonensis result in the lack of sensitivity to end-product inhibition. Biochem. Biophys. Res. Commun. 266:162-166.
10. Lange, C., D. Rittmann, V. F. Wendisch, M. Bott, and H. Sahm. 2003. Global expression profiling and physiological characterization of Corynebacterium glutamicum grown in the presence of L-valine. Appl. Environ. Microbiol. 69:2521-2532.
11. Leyval, D., D. Uy, S. Delaunay, J. L. Goergen, and J. M. Engasser. 2003. Characterisation of the enzyme activities involved in the valine biosynthetic pathway in a valine-producing strain of Corynebacterium glutamicum. J. Biotechnol. 104:241-252.
12. Lindroth, P., and K. Mopper. 1979. High performance liquid Chromatographic determination of subpicomole amounts of amino acids by precolumn fluorescence derivatization with o-phthalaldehyde. Anal. Chem. 51:1667-1674.
13. Mendel, S., T. Elkayam, C. Sella, V. Vinogradov, M. Vyazmensky, D. M. Chipman, and Z. Barak. 2001. Acetohydroxyacid synthase: a proposed structure for regulatory subunits supported by evidence from mutagenesis. J. Mol. Biol. 307:405-477.
14. Morbach, S., C. Junger, H. Sahm, and L. Eggeling. 2000. Attenuation control of ilvBNC in Corynebacterium glutamicum: evidence of leader peptide formation without the presence of a ribosome binding site. J. Biosci. Bioeng. 90:501-507.
15. Pátek, M., J. Hochmannová, and J. Nesvera. 1993. Production of threonine by Brevibacteriitm flavum containing threonine biosynthesis genes from Escherichia coli Fol. Microbiol. 38:355-359.
16. Pospíšil, S., J. Kopecky, V. Přikrylová, and J. Spížek. 1999. Overproduction of 2-ketoisovalerate and monensin production by regulatory mutants of Streptomyces cinnamonensis resistant to 2-ketobutyrate and amino acids. FEMS Microbiol. Lett. 172:197-204.
17. Pridmore, R. D. 1987. New and versatile cloning vectors with kanamycin-resistance marker. Gene 56:309-312.
18. Radmacher, E., A. Vaitsikova, U. Burger, K. Krumbach, H. Sahm, and L. Eggeling. 2002. Linking central metabolism with increased pathway flux: L-valine accumulation by Corynebacterium glutamicum. Appl. Environ. Microbiol. 68:2246-2250.
19. Reinscheid, D. J., W. Kronemeyer, L. Eggeling, B. J. Eikmanns, and H. Sahm. 1994. Stable expression of hom-1-thrB in Corynebacterium glutamicum and its effect on the carbon flux to threonine and related amino acids. Appl. Environ. Microbiol. 60:126-132.
20. Sahm, H., and L. Eggeling. 1999. D-Pantothenate synthesis in Corynebacterium glutamicum and use of panBC and genes encoding L-valine synthesis for D-pantothenate overproduction. Appl. Environ. Microbiol. 65:1973-1979.
21. Sambrook, J., and D. V. Russel. 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
22. Santamaría, R., J. A. Gil, J. M. Mesas, and J. F. Martin. 1984. Characterization of endogenous plasmid and development of cloning vectors and a transformation system in Brevibacterium lactofementum. J. Gen. Microbiol. 130:2237-2246.
23. Schäfer, A., A. Tauch, W. Jäger, J. Kalinowski, G. Thierbach, and A. Pühler. 1994. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 145:69-73.
24. Tsuchida, T., and H. Momose. 1975. Genetic changes of regulatory mechanisms occurred in leucine and valine producing mutants derived from Brevibacterium lactofermentum. Agric. Biol. Chem. 39:2193-2198.
25. Tsuchida, T., and H. Momose. 1986. Improvement of an L-leucine-producing mutant of Brevibacterium lactofermentum 2256 by genetically desensitizing it to α-acetohydroxy acid synthase. Appl. Environ. Microbiol. 51:1024-1027.
26. Umbarger, H. E. 1996. Biosynthesis of the branched-chain amino acids, p. 442-457. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed. ASM Press, Washington, D.C.
27. van der Rest, M. E., C. Lange, and D. Molenaar. 1999. A heat shock following electroporation induces highly efficient transformation of Corynebacterium glutamicum with xenogeneic plasmid DNA. Appl. Microbiol. Biotechnol. 52:541-545.
28. Vyazmensky, M., C. Sella, Z. Barak, and D. M. Chipman. 1996. Isolation and characterization of subunits of acetohydroxy acid synthase isozyme III and reconstitution of the holoenzyme. Biochemistry 35:10339-10346.
29. Wang, Q., and J. M. Calvo. 1993. Lrp, a global regulatory protein of Escherichia coli, binds co-operatively to multiple sites and activates transcription of ilvIH. J. Mol. Biol. 229:306-318.
30. Westerfeld, W. W. 1945. A colorimetric detection of blood acetoin. J. Biol. Chem. 161:495-502.