Alleviating redox imbalance enhances 7-dehydrocholesterol production in engineered saccharomyces cerevisiae

PLoS ONE

Volumn 10, Issue 6, 2015, Pages

  Su, Wan ^a   Xiao, Wen Hai ^a   Wang, Ying ^a   Liu, Duo ^a   Zhou, Xiao ^a   Yuan, Ying Jin ^a  

^a TIANJIN UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

7 DEHYDROCHOLESTEROL; ALCOHOL; ERGOSTEROL; GLYCEROL; NICOTINAMIDE ADENINE DINUCLEOTIDE; PROTEIN AOX1; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEHYDROGENASE; UNCLASSIFIED DRUG; 7-DEHYDROCHOLESTEROL; CYTOCHROME P450; ERG5 PROTEIN, S CEREVISIAE; PRIMER DNA; SACCHAROMYCES CEREVISIAE PROTEIN;

ARTICLE; BIOREACTOR; CONCENTRATION (PARAMETERS); CYTOSOL; ERG5 GENE; FUNGAL GENE; GENE DELETION; GENETIC CODE; NONHUMAN; OXIDATION REDUCTION STATE; PROTEIN ENGINEERING; PROTEIN SYNTHESIS; SACCHAROMYCES CEREVISIAE; SIGNAL TRANSDUCTION; BIOSYNTHESIS; FERMENTATION; GENE INACTIVATION; GENETIC ENGINEERING; GENETICS; METABOLISM; MICROBIOLOGY; OXIDATION REDUCTION REACTION; PHYSIOLOGY; PLASMID; POLYMERASE CHAIN REACTION; PROCEDURES; TRANSGENIC ORGANISM;

SACCHAROMYCES CEREVISIAE;

BIOSYNTHETIC PATHWAYS; CYTOCHROME P-450 ENZYME SYSTEM; DEHYDROCHOLESTEROLS; DNA PRIMERS; ERGOSTEROL; FERMENTATION; GENE KNOCKOUT TECHNIQUES; GENETIC ENGINEERING; INDUSTRIAL MICROBIOLOGY; ORGANISMS, GENETICALLY MODIFIED; OXIDATION-REDUCTION; PLASMIDS; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 84939242519     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0130840     Document Type: Article

References (44)

1. Jain VK, Divol B, Prior BA, Bauer FF (2012) Effect of alternative NAD+-regenerating pathways on the formation of primary and secondary aroma compounds in a Saccharomyces cerevisiae glycerol-defective mutant. Appl Microbiol Biotechnol 93: 131-141. doi: 10.1007/s00253-011-3431-z PMID: 21720823
2. Wang Y, San KY, Bennett GN (2013) Cofactor engineering for advancing chemical biotechnology. Curr Opin Biotechnol 24: 994-999. doi: 10.1016/j.copbio.2013.03.022 PMID: 23611567
3. King ZA, Feist AM (2014) Optimal cofactor swapping can increase the theoretical yield for chemical production in Escherichia coli and Saccharomyces cerevisiae. Metab Eng 24: 117-128. doi: 10.1016/j.ymben.2014.05.009 PMID: 24831709
4. Vemuri GN, Eiteman MA, McEwen JE, Olsson L, Nielsen J (2007) Increasing NADH oxidation reduces overflow metabolism in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 104: 2402-2407. PMID: 17287356
5. Chen X, Li S, Liu L (2014) Engineering redox balance through cofactor systems. Trends Biotechnol 32: 337-343. doi: 10.1016/j.tibtech.2014.04.003 PMID: 24794722
6. Opgenorth PH, Korman TP, Bowie JU (2014) A synthetic biochemistry molecular purge valve module that maintains redox balance. Nat Commun 5: 4113. doi: 10.1038/ncomms5113 PMID: 24936528
7. Chenault HK, Simon ES, Whitesides GM (1988) Cofactor Regeneration for Enzyme-Catalysed Synthesis. Biotechnol Genet Eng Rev 6: 221-270. PMID: 3071373
8. San KY, Bennett GN, Berríos-Rivera SJ, Vadali RV, Yang YT, Horton E, et al. (2002) Metabolic engineering through cofactor manipulation and its effects on metabolic flux redistribution in Escherichia coli. Metab Eng 4: 182-192. PMID: 12009797
9. Guo T, Kong J, Zhang L, Zhang C, Hu S (2012) Fine tuning of the lactate and diacetyl production through promoter engineering in Lactococcus lactis. PLoS One 7: e36296. doi: 10.1371/journal.pone.0036296 PMID: 22558426
10. Bikle DD (2014) Vitamin D metabolism, mechanism of action, and clinical applications. Chem Biol 21: 319-329. doi: 10.1016/j.chembiol.2013.12.016 PMID: 24529992
11. Confalone PN, Kulesha ID, Uskokovic MR (1981) A new synthesis of 7-dehydrocholesterol. J Org Chem 46: 1030-1032.
12. Lewis D, Galczenski H, Needle S, Tang SY, Amin D, Gleason M, et al. (1995) Enzyme inhibition during the conversion of squalene to cholesterol. Steroids 60: 475-483. PMID: 7482633
13. Rudolph PH, Spaziani E, Wang WL (1992) Formation of ecdysteroids by Y-organs of the crab, Menippe mercenaria: I. Biosynthesis of 7-dehydrocholesterol in vivo. Gen Comp Endocrinol 88: 224-234. PMID: 1478439
14. Rudolph PH, Spaziani E (1992) Formation of ecdysteroids by Y-organs of the crab, Menippe mercenaria: II. Incorporation of cholesterol into 7-dehydrocholesterol and secretion products in vitro. Gen Comp Endocrinol 88: 235-242. PMID: 1478440
15. Lang C (2011) Preparation of 7-dehydrocholesterol and/or the biosynthetic intermediates and/or secondary products thereof in transgenic organisms. US Patent.
16. Lian J, Si T, Nair NU, Zhao H (2014) Design and construction of acetyl-CoA overproducing Saccharomyces cerevisiae strains. Metab Eng 24: 139-149. doi: 10.1016/j.ymben.2014.05.010 PMID: 24853351
17. Auzat I, Chapuy-regaud S, Santos DD, Ogunniyi AD, Thomas IL, Garel JR, et al. (1999) The NADH oxidase of Streptococcus pneumoniae: its involvement in competence and virulence. Mol Microbiol 34: 1018-1028. PMID: 10594826
18. Johnson CH, Prigge JT, Warren AD, McEwen JE (2003) Characterization of an alternative oxidase activity of Histoplasma capsulatum. Yeast 20: 381-388. PMID: 12673621
19. Sun J, Shao Z, Zhao H, Nair N, Wen F, Xu JH, et al. (2012) Cloning and characterization of a panel of constitutive promoters for applications in pathway engineering in Saccharomyces cerevisiae. Biotechnol Bioeng 109: 2082-2092. doi: 10.1002/bit.24481 PMID: 22383307
20. Lin Q, Jia B, Mitchell LA, Luo J, Yang K, Zeller KI, et al. (2015) RADOM, an efficient in vivo method for assembling designed DNA fragments up to 10 kb long in Saccharomyces cerevisiae. ACS Synth Biol 4 (3): 213-220. doi: 10.1021/sb500241e PMID: 24895839
21. Canelas B, GulikWMVan, Heijnen JJ (2008) Determination of the Cytosolic Free NAD/NADH Ratio in Saccharomyces cerevisiae Under Steady-State and Highly Dynamic Conditions. Biotechnol Bioeng 100: 734-743. doi: 10.1002/bit.21813 PMID: 18383140
22. Canelas AB, Ras C, ten Pierick A, van Dam JC, Heijnen JJ, van Gulik WM. (2008) Leakage-free rapid quenching technique for yeast metabolomics. Metabolomics 4: 226-239.
23. Wang X, Jin M, Balan V, Jones a. D, Li X, Li BZ, et al. (2014) Comparative metabolic profiling revealed limitations in xylose-fermenting yeast during co-fermentation of glucose and xylose in the presence of inhibitors. Biotechnol Bioeng 111: 152-164. PMID: 24404570
24. Kato H, Izumi Y, Hasunuma T, Matsuda F, Kondo A (2012) Widely targeted metabolic profiling analysis of yeast central metabolites. J Biosci Bioeng 113: 665-673. doi: 10.1016/j.jbiosc.2011.12.013 PMID: 22280965
25. Polakowski T, Stahl U, Lang C (1998) Overexpression of a cytosolic hydroxymethylglutaryl-CoA reductase leads to squalene accumulation in yeast. Appl Microbiol Biotechnol 49: 66-71. PMID: 9487712
26. Nielsen J (1998) The role of metabolic engineering in the production of secondary metabolites. Curr Opin Microbiol 1: 330-336. PMID: 10066494
27. Nielsen J (2014) Synthetic Biology for Engineering Acetyl Coenzyme A Metabolism in Yeast. MBio 5: 14-16.
28. Grabin K (2002) Dolichol biosynthesis in the yeast Saccharomyces cerevisiae: an insight into the regulatory role of farnesyl diphosphate synthase. FEMS Yeast Res 2: 259-265. PMID: 12702274
29. Shiba Y, Paradise EM, Kirby J, Ro DK, Keasling JD (2007) Engineering of the pyruvate dehydrogenase bypass in Saccharomyces cerevisiae for high-level production of isoprenoids. Metab Eng 9: 160-168. PMID: 17196416
30. Strijbis K, Distel B (2010) Intracellular acetyl unit transport in fungal carbon metabolism. Eukaryot Cell 9: 1809-1815. doi: 10.1128/EC.00172-10 PMID: 20889721
31. Chen Y, Daviet L, Schalk M, Siewers V, Nielsen J (2013) Establishing a platform cell factory through engineering of yeast acetyl-CoA metabolism. Metab Eng 15: 48-54. doi: 10.1016/j.ymben.2012.11. 002 PMID: 23164578
32. De Jong BW, Shi S, Siewers V, Nielsen J (2014) Improved production of fatty acid ethyl esters in Saccharomyces cerevisiae through up-regulation of the ethanol degradation pathway and expression of the heterologous phosphoketolase pathway. Microb Cell Fact 13: 39. doi: 10.1186/1475-2859-13-39 PMID: 24618091
33. Arabidopsis AA, Fatland BL, Ke J, Anderson MD, Mentzen WI, Cui LW, et al. (2002) Molecular Characterization of a Heteromeric ATP-Citrate Lyase That Generates Cytosolic. Plant Physiol 130: 740-756. PMID: 12376641
34. Tang X, Feng H, Chen WN (2013) Metabolic engineering for enhanced fatty acids synthesis in Saccharomyces cerevisiae. Metab Eng 16: 95-102. doi: 10.1016/j.ymben.2013.01.003 PMID: 23353549
35. Påhlman IL, Gustafsson L, Rigoulet M, Larsson C (2001) Cytosolic redox metabolism in aerobic chemostat cultures of Saccharomyces cerevisiae. Yeast 18: 611-620. PMID: 11329172
36. De Deken R (1966) The Crabtree Effects: A Regulatory System in Yeast. J Gen Microbiol 44: 149-156. PMID: 5969497
37. Ding JM, Huang XW, Zhang LM, Zhao N, Yang DM, Zhang KQ. (2009) Tolerance and stress response to ethanol in the yeast Saccharomyces cerevisiae. Appl Microbiol Biotechnol 85: 253-263. doi: 10. 1007/s00253-009-2223-1 PMID: 19756577
38. Wriessnegger T, Pichler H (2013) Yeast metabolic engineering-targeting sterol metabolism and terpenoid formation. Prog Lipid Res 52: 277-293. doi: 10.1016/j.plipres.2013.03.001 PMID: 23567752
39. Yang H, Tong J, Lee CW, Ha S, Eom SH, Im YJ. (2015) Structural mechanism of ergosterol regulation by fungal sterol transcription factor Upc2. Nat Commun 6: 6129. doi: 10.1038/ncomms7129 PMID: 25655993
40. Kristan K, Rižner TL (2012) Steroid-transforming enzymes in fungi. J Steroid Biochem Mol Biol 129: 79-91. doi: 10.1016/j.jsbmb.2011.08.012 PMID: 21946531
41. Berríos-Rivera SJ, Bennett GN, San KY (2002) Metabolic Engineering of Escherichia coli: Increase of NADH Availability by Overexpressing an NAD+-Dependent Formate Dehydrogenase. Plasmid 229: 217-229.
42. Berríos-Rivera SJ, Bennett GN, San KY (2002) The effect of increasing NADH availability on the redistribution of metabolic fluxes in Escherichia coli chemostat cultures. Metab Eng 4: 230-237. PMID: 12616692
43. Zhou YJ, Yang W, Wang L, Zhu ZW, Zhang SF, Zhao ZB. (2013) Engineering NAD+ availability for Escherichia coli whole-cell biocatalysis: a case study for dihydroxyacetone production. Microb Cell Fact 12: 103. doi: 10.1186/1475-2859-12-103 PMID: 24209782
44. Salusjärvi L, Kaunisto S, Holmström S, Vehkomäki ML, Koivuranta K, Pitkänen JP, et al. (2013) Overexpression of NADH-dependent fumarate reductase improves D-xylose fermentation in recombinant Saccharomyces cerevisiae. J Ind Microbiol Biotechnol 40: 1383-1392. doi: 10.1007/s10295-013-1344-9 PMID: 24113892