Expression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies

Journal of Structural and Functional Genomics

Volumn 16, Issue 2, 2015, Pages 67-80

  Aceti, David J ^a   Bingman, Craig A ^a,c   Wrobel, Russell L ^a   Frederick, Ronnie O ^a   Makino, Shin ichi ^a   Nichols, Karl W ^a   Sahu, Sarata C ^a   Bergeman, Lai F ^a   Blommel, Paul G ^a   Cornilescu, Claudia C ^a   Gromek, Katarzyna A ^a   Seder, Kory D ^a   Hwang, Soyoon ^a   Primm, John G ^a   Sabat, Grzegorz ^a   Vojtik, Frank C ^a   Volkman, Brian F ^b   Zolnai, Zsolt ^a   Phillips, George N ^a,c   Markley, John L ^a   Fox, Brian G ^a   more..

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b MEDICAL COLLEGE OF WISCONSIN   (United States)

^c RICE UNIVERSITY   (United States)

Author keywords

Flexi Vector cloning; Gateway cloning; Maltose Binding Protein solubility tag; NIH Protein Structure Initiative; Structural genomics; Wheat germ cell free translation

Indexed keywords

PROTEIN;

CELL FREE SYSTEM; ESCHERICHIA COLI; EUKARYOTE; GENE EXPRESSION; GENE VECTOR; GENETICS; GERM CELL; ISOLATION AND PURIFICATION; MOLECULAR CLONING; PROTEIN SYNTHESIS; WHEAT;

CELL-FREE SYSTEM; CLONING, MOLECULAR; ESCHERICHIA COLI; EUKARYOTA; GENE EXPRESSION; GENETIC VECTORS; GERM CELLS; PROTEIN BIOSYNTHESIS; PROTEINS; TRITICUM;

EID: 84937761852     PISSN: 1345711X     EISSN: 15700267     Source Type: Journal    
DOI: 10.1007/s10969-015-9198-1     Document Type: Article

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