An expression vector tailored for large-scale, high-throughput purification of recombinant proteins

Protein Expression and Purification

Volumn 47, Issue 2, 2006, Pages 446-454

  Donnelly, Mark I ^a   Zhou, Min ^a   Millard, Cynthia Sanville ^a   Clancy, Shonda ^a   Stols, Lucy ^a   Eschenfeldt, William H ^a   Collart, Frank R ^a   Joachimiak, Andrzej ^a  

^a ARGONNE NATIONAL LABORATORY   (United States)

Author keywords

High throughput; Ligation independent cloning; Maltose binding protein; Structural genomics; TVMV protease

Indexed keywords

CARRIER PROTEIN; HYBRID PROTEIN; MALTOSE BINDING PROTEIN; MALTOSE-BINDING PROTEIN;

AFFINITY CHROMATOGRAPHY; ANIMAL; ARTICLE; BIOSYNTHESIS; ESCHERICHIA COLI; GENE VECTOR; GENETICS; HUMAN; ISOLATION AND PURIFICATION; PROTEIN TERTIARY STRUCTURE;

ANIMALS; CARRIER PROTEINS; CHROMATOGRAPHY, AFFINITY; ESCHERICHIA COLI; GENETIC VECTORS; HUMANS; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS;

TOBACCO VEIN MOTTLING VIRUS;

EID: 33646579179     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.12.011     Document Type: Article

References (26)

1. DiDonato M., Deacon A.M., Klock H.E., McMullan D., and Lesley S.A. A scaleable and integrated crystallization pipeline applied to mining the Thermotoga maritima proteome. J. Struct. Funct. Genomics 5 (2004) 133-146
2. Braun P., Hu Y., Shen B., Halleck A., Koundinya M., Harlow E., and LaBaer J. Proteome-scale purification of human proteins from bacteria. Proc. Natl. Acad. Sci. USA 99 (2002) 2654-2659
3. Dyson M.R., Shadbolt S.P., Vincent K.J., Perera R.L., and McCafferty J. Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression. BMC Biotechnol. 4 (2004) 32
4. Jeon W.B., Aceti D.J., Bingman C.A., Vojtik F.C., Olson A.C., Ellefson J.M., McCombs J.E., Sreenath H.K., Blommel P.G., Seder K.D., Burns B.T., Geetha H.V., Harms A.C., Sabat G., Sussman M.R., Fox B.G., and Phillips Jr. G.N. High-throughput purification and quality assurance of Arabidopsis thaliana proteins for eukaryotic structural genomics. J. Struct. Funct. Genomics 5 (2004)
5. Yee A., Pardee K., Christendat D., Savchenko A., Edwards A.M., and Arrowsmith C.H. Structural proteomics: toward high-throughput structural biology as a tool in functional genomics. Acc. Chem. Res. 36 (2003) 183-189
6. Kim Y., Dementieva I., Zhou M., Wu R., Lezondra L., Quartey P., Joachimiak G., Korolev O., Li H., and Joachimiak A. Automation of protein purification for structural genomics. J. Struct. Funct. Genomics 5 (2004) 111-118
7. Braun P., and LaBaer J. High throughput protein production for functional proteomics. Trends Biotechnol. 21 (2003) 383-388
8. Porath J. Immobilized metal ion affinity chromatography. Protein Expr. Purif. 3 (1992) 263-281
9. Fox J.D., and Waugh D.S. Maltose-binding protein as a solubility enhancer. Methods Mol. Biol. 205 (2003) 99-117
10. Kapust R.B., and Waugh D.S. Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused. Protein Sci. 8 (1999) 1668-1674
11. Dougherty W.G., Carrington J.C., Cary S.M., and Parks T.D. Biochemical and mutational analysis of a plant virus polyprotein cleavage site. EMBO J. 7 (1988) 1281-1287
12. Parks T.D., Leuther K.K., Howard E.D., Johnston S.A., and Dougherty W.G. Release of proteins and peptides from fusion proteins using a recombinant plant virus proteinase. Anal. Biochem. 216 (1994) 413-417
13. Stols L., Gu M., Dieckman L., Raffen R., Collart F.R., and Donnelly M.I. A new vector for high-throughput, ligation-independent cloning encoding a tobacco etch virus protease cleavage site. Protein Expr. Purif. 25 (2002) 8-15
14. Kapust R.B., and Waugh D.S. Controlled intracellular processing of fusion proteins by TEV protease. Protein Expr. Purif. 19 (2000) 312-318
15. Nallamsetty S., Kapust R.B., Tozser J., Cherry S., Tropea J.E., Copeland T.D., and Waugh D.S. Efficient site-specific processing of fusion proteins by tobacco vein mottling virus protease in vivo and in vitro. Protein Expr. Purif. 38 (2004) 108-115
16. Yoon H.Y., Hwang D.C., Choi K.Y., and Song B.D. Proteolytic processing of oligopeptides containing the target sequences by the recombinant tobacco vein mottling virus NIa proteinase. Mol. Cells 10 (2000) 213-219
17. Kapust R.B., Tozser J., Fox J.D., Anderson D.E., Cherry S., Copeland T.D., and Waugh D.S. Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency. Protein Eng. 14 (2001) 993-1000
18. Doublie S. Preparation of selenomethionyl proteins for phase determination. Methods Enzymol. 276 (1997) 523-530
19. Van Duyne G.D., Standaert R.F., Karplus P.A., Schreiber S.L., and Clardy J. Atomic structures of the human immunophilin FKBP-12 complexes with FK506 and rapamycin. J. Mol. Biol. 229 (1993) 105-124
20. Millard C.S., Stols L., Quartey P., Kim Y., Dementieva I., and Donnelly M.I. A less laborious approach to the high-throughput production of recombinant proteins in Escherichia coli using 2-liter plastic bottles. Protein Expr. Purif. 29 (2003) 311-320
21. Stols L., Millard C.S., Dementieva I., and Donnelly M.I. Production of selenomethionine-labeled proteins in two-liter plastic bottles for structure determination. J. Struct. Funct. Genomics 5 (2004) 95-102
22. Whitman W.B., Ankwanda E., and Wolfe R.S. Nutrition and carbon metabolism of Methanococcus voltae. J. Bacteriol. 149 (1982) 852-863
23. Studier F.W. Protein production by auto-induction in high density shaking cultures. Protein Expr. Purif. 41 (2005) 207-234
24. 15N]-labeled proteins for NMR screening and structure determination. Protein Expr. Purif. 40 (2005) 268-278
25. Sreenath H.K., Bingman C.A., Buchan B.W., Seder K.D., Burns B.T., Geetha H.V., Jeon W.B., Vojtik F.C., Aceti D.J., Frederick R.O., Phillips Jr. G.N., and Fox B.G. Protocols for production of selenomethionine-labeled proteins in 2-L polyethylene terephthalate bottles using auto-induction medium. Protein Expr. Purif. 40 (2005) 256-267
26. Moy S., Dieckman L., Schiffer M., Maltsev N., Yu G.X., and Collart F.R. Genome-scale expression of proteins from Bacillus subtilis. J. Struct. Funct. Genomics 5 (2004) 103-109