Engineering subtilisin into a fluoride-triggered processing protease useful for one-step protein purification

Biochemistry

Volumn 43, Issue 46, 2004, Pages 14539-14546

  Ruan, Biao ^a   Fisher, Kathryn E ^a   Alexander, Patrick A ^a   Doroshko, Viktoriya ^a   Bryan, Philip N ^a  

^a UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORINE COMPOUNDS; IMPURITIES; MUTAGENESIS; OPTIMIZATION; PURIFICATION; ROBUSTNESS (CONTROL SYSTEMS);

FLUORIDE-TRIGGERED PROCESSING; PROCESSING REACTIONS; RECOGNITION SEQUENCES; SUBTILISIN;

PROTEINS;

ANION; FLUORIDE ION; HYBRID PROTEIN; MUTANT PROTEIN; PROTEINASE; SUBTILISIN;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME IMMOBILIZATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE COMPLEX; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN ENGINEERING; PROTEIN PURIFICATION;

AMINO ACID SUBSTITUTION; BACILLUS SUBTILIS; ENZYME STABILITY; FLUORIDES; HYDROLYSIS; KINETICS; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; SUBTILISINS;

EID: 8744294716     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi048177j     Document Type: Article

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