Libyan Thymus capitatus essential oil: Antioxidant, antimicrobial, cytotoxic and colon pathogen adhesion-inhibition properties

Journal of Applied Microbiology

Volumn 119, Issue 2, 2015, Pages 389-399

  Džamić, Ana M ^a   Nikolic B J ^a   Giweli, A A ^a   Mitic Culafic D S ^a   Sokovic M D ^a   Ristic M S ^b   Knezevic Vukcevic J B ^a   Marin, P D ^a  

^a UNIVERSITY OF BELGRADE   (Serbia)

^b Institute for Medicinal Plant Research Dr Josif Pan i   (Serbia)

Author keywords

Antimicrobial activity; Antioxidant; Cytotoxicity; Essential oil; Thymus capitatus

Indexed keywords

ADHESION; CELL CULTURE; CYTOTOXICITY; ESCHERICHIA COLI; ESSENTIAL OILS; PATHOGENS; PHENOLS; THYMUS;

ANTI-MICROBIAL ACTIVITY; ANTIBACTERIALS; ANTIOXIDANT PROPERTIES; BIOLOGICAL PROPERTIES; COLONISATION; CYTOTOXIC; DIPHENYLPICRYLHYDRAZYL; INHIBITION PROPERTY; MICRODILUTION; THYMUS CAPITATU;

ANTIOXIDANTS;

1,1 DIPHENYL 2 PICRYLHYDRAZYL; 2,2 DIPHENYLPICRYLHYDRAZYL; CARVACROL; ESSENTIAL OIL; THYMOL; UNCLASSIFIED DRUG; ANTIINFECTIVE AGENT; ANTIOXIDANT; PLANT EXTRACT;

ADHESION; ANTIMICROBIAL ACTIVITY; ANTIOXIDANT; BIOASSAY; BIOCHEMICAL COMPOSITION; CELLS AND CELL COMPONENTS; COLONIZATION; DICOTYLEDON; ESSENTIAL OIL; HOMINID; INHIBITION; MICROBIOLOGY; PATHOGEN; TOXICITY; WILD POPULATION;

ADHESION; ANTIMICROBIAL ACTIVITY; ANTIOXIDANT ACTIVITY; ARTICLE; ASSAY; BACTERIUM ADHERENCE; COLON; CONTROLLED STUDY; CYTOTOXICITY; ESCHERICHIA COLI; HUMAN; HUMAN CELL; IC50; LISTERIA MONOCYTOGENES; MTT ASSAY; CHEMISTRY; DRUG EFFECTS; FUNGUS; GROWTH, DEVELOPMENT AND AGING; MICROBIOLOGY; PHYSIOLOGY; THYMUS;

LIBYAN ARAB JAMAHIRIYA;

ESCHERICHIA COLI; FUNGI; LISTERIA MONOCYTOGENES; THYMUS CAPITATUS;

ANTI-INFECTIVE AGENTS; ANTIOXIDANTS; BACTERIAL ADHESION; COLON; ESCHERICHIA COLI; FUNGI; HUMANS; LISTERIA MONOCYTOGENES; OILS, VOLATILE; PLANT EXTRACTS; THYMUS GLAND;

EID: 84937637951     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/jam.12864     Document Type: Article

References (51)

1. Adam, K., Sivropoulu, A., Kokkini, S., Lanaras, T. and Arsenakis, M. (1998) Antifungal activities of Origanum vulgare subsp. hirtum, Mentha spicata, Lavandula angustifolia and Salvia fruticosa essential oils against human pathogenic fungi. J Agric Food Chem 46, 1739-1745.
2. Adams, R.P. (2007) Identification of Essential Oil Components by Gas chromatography/Mass Spectrometry, 4th edn. Carol Stream, IL: Allured Publ. Corp.
3. Adams, S., Kunz, B. and Weidenbörner, M. (1996) Mycelial deformations of Cladosporium herbarum due to the application of eugenol and carvacrol. J Essent Oil Res 8, 535-540.
4. Akalini, G. and Incesu, Z. (2011) The effect of carvacrol on apoptosis of H-RAS and N-RAS transformed cell lines. Turk J Pharm Sci 8, 105-116.
5. van Alphen, L.B., Burt, S.A., Veenendaal, A.K.J., Bleumink-Pluym, N.M.C. and van Putten, J.P.M. (2012) The natural antimicrobial carvacrol inhibits Campylobacter jejuni motility and infection of epithelial cells. PLoS ONE 7, e45343.
6. Bakkali, F., Averbeck, S., Averbeck, D. and Idaomar, M. (2008) Biological effects of essential oils - A Rewiev. Food Chem Toxicol 46, 446-475.
7. Blois, M.S. (1958) Antioxidant determinations by the use of a stable free radical. Nature 181, 1199-1200.
8. Bouzouita, N., Kachouri, F., Hamdi, M. and Chaabouni, M.M. (2003) Antimicrobial activity of essential oils from Tunisian aromatic plants. Flavour Fragr J 18, 380-383.
9. Burt, S. (2004) Essential oils: their antibacterial properties and potential applications in foods - A review. Int J Food Microbiol 94, 223-253.
10. Clinical and Laboratory Standards Institute (2009) Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically. Approved standard, 8th edn. CLSI publication M07-A8. Wayne, PA: Clinical and Laboratory Standards Institute.
11. Dal Sasso, M., Culici, M., Pier, C., Braga, P.C., Guffanti, E.E. and Mucci, M. (2006) Thymol: inhibitory activity on Escherichia coli and Staphylococcus aureus adhesion to human vaginal cells. J Essent Oil Res 18, 455-461.
12. Darvishi, E., Omidi, M., Bushehri, A.A., Golshani, A. and Smith, M.L. (2013) Thymol antifungal mode of action involves telomerase inhibition. Med Mycol 51, 826-834.
13. El Ajjouri, M., Satrani, B., Ghanmi, M., Aafi, A., Farah, A., Rahouti, M., Amarti, F. and Aberchane, M. (2008) Activité antifongique des huiles essentielles de Thymus bleicherianus Pomel et Thymus capitatus (L.) Hoffm. & Link contre les champignons de pourriture du bois d'ouvre. Biotechnol Agron Soc Environ 12, 345-351.
14. El-Nekeety, A.A., Mohamed, S.R., Hathout, A.S., Hassan, N.S., Aly, S.E. and Abdel-Wahhab, M.A. (2011) Antioxidant properties of Thymus vulgaris oil against aflatoxin-induced oxidative stress in male rats. Toxicon 57, 984-991.
15. Espinel-Ingroff, A. (2001) Comparasion of the E-test with the NCCLS M38-P method for antifungal susceptibility testing of common and emerging pathogenic filamentous fungi. J Clin Microbiol 39, 1360-1367.
16. Faleiro, L., Miguel, G., Gomes, S., Costa, L., Venâncio, F., Teixeira, A., Figueiredo, A.C., Barroso, J.G. et al. (2005) Antibacterial and antioxidant activities of essential oils isolated from Thymbra capitata L. (Cav.) and Origanum vulgare L. J Agr Food Chem 53, 8162-8168.
17. Figueiredo, C., Barroso, J.G., Pedro, L.G., Salgueiro, L., Miguel, M.G. and Faleiro, M.L. (2008) Portuguese Thymbra and Thymus Species Volatiles: chemical composition and biological activities. Curr Pharm Des 14, 3120-3140.
18. Galego, L., Almeida, V., Gonçalves, V., Costa, M., Monteiro, I., Matos, F. and Miguel, G. (2008) Antioxidant activity of the essential oils of Thymbra capitata, Origanum vulgare, Thymus mastichina and Calamintha baetica. Acta Hortic 765, 325-334.
19. Gholijani, N., Gharagozloo, M., Farjadian, S. and Amirghofran, Z. (2015) Modulatory effects of thymol and carvacrol on inflammatory transcription factors in lipopolysaccharide-treated macrophages. J Immunotoxicol 27, 1-8.
20. Ghrairi, T. and Hani, K. (2015) Enhanced bactericidal effect of enterocin A in combination with thyme essential oils against L. monocytogenes and E. coli O157:H7. J Food Sci Technol 52, 2148-2156.
21. Goren, A.C., Bilsel, G., Bilsel, M., Demir, H. and Kocabas, E.E. (2003) Analysis of essential oil of Coridothymus capitatus (L.) and its antibacterial and antifungal activity. Z Naturforsch C 58, 687-690.
22. Hanel, H. and Raether, W. (1988) A more sophisticated method of determining the fungicidal effect of water-insoluble preparations with a cell harvester, using miconazole as an example. Mycoses 31, 148-154.
23. Hansen, M.B., Nielsen, S.E. and Berg, K. (1989) Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. Immunol Methods 119, 203-210.
24. Hazzit, M., Baaliouamer, A., Verissimo, A.R., Faleiro, M.L. and Miguel, M.G. (2009) Chemical composition and biological activities of Algerian Thymus oils. Food Chem 116, 714-721.
25. Hedhilia, L., Romdhane, M., Planche, H. and Abderrabba, M. (2005) Towards gas chromatography-mass spectrometry coupling protocols for both identifying and quantification essential oils of Thymus capitatus Hoff et Link. J Chromatogr A 1064, 129-134.
26. Jafri, S.M.H. and El-gadi, A. (1985) Flora of Libya Vol (118). Al Faateh University. Faculty of Science. Tripoli, Libya: Department of Botany.
27. Kordali, S., Cakir, A., Ozer, H., Cakmakci, R., Kesdek, M. and Mete, E. (2008) Antifungal, phytotoxic and insecticidal properties of essential oil isolated from Turkish Origanum acutidens and its three components, carvacrol, thymol and p-cymene. Bioresour Technol 99, 8788-8795.
28. Le Floch, E. and Boulos, L. (2008) Flora of Tunisia Synonymous Reviewed Catalog, Montepellier. Tunisia: Le Flosh.
29. 2+] rise and apoptosis in human glioblastoma cells. Life Sci 90, 703-711.
30. Llana-Ruiz-Cabello, M., Gutiérrez-Praena, D., Pichardo, S., Moreno, F.J., Bermúdez, J.M., Aucejo, S. and Cameán, A.M. (2014) Cytotoxicity and morphological effects induced by carvacrol and thymolon the human cell line Caco-2. Food Chem Toxicol 64, 281-290.
31. Llana-Ruiz-Cabello, M., Gutiérrez-Praena, D., Puerto, M., Pichardo, S., Jos, Á. and Cameán, A.M. (2015) In vitro pro-oxidant/antioxidant role of carvacrol, thymol and their mixture in the intestinal Caco-2 cell line. Toxicol In Vitro 29, 647-656.
32. Mathela, C.S., Singh, K.K. and Gupta, V.K. (2010) Synthesis and in vitro antibacterial activity of thymol and carvacrol derivatives. Acta Pol Pharm 67, 375-380.
33. Miceli, A., Negro, C. and Tommasi, L. (2006) Essential oil variability in Thymbra capitata (L.) Cav. growing wild in Southren Apulia (Italy). Biochem Syst Ecol 34, 528-535.
34. Miguel, M.G., Simoes, M.F., Figueiredo, A.C., Barroso, J.G. and Carvalho, L.M. (2005) Evaluation of the antioxidant activity of Thymus capitatus, Thymus mastichina and Thymus camphoratus essential oil. J Food Lipids 12, 181-197.
35. Morales, R. (1989) The genus Thymus L. in the western Mediterranean region (Lamiaceae). Biocosme Mésogéen 6, 205-211.
36. Morales, R. (2002) The history, botany and taxonomy of the genus Thymus. In Thyme: The Genus Thymus ed. Stahl-Biskup, E., Sáez, F. pp. 1-43. London and New York: Taylor and Francis.
37. Moreno, S., Scheyer, T., Romano, C. and Vojnov, A. (2006) Antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition. Free Radical Res 40, 223-231.
38. Nohynek, L.J., Alakomi, H.-L., Kähkönen, M.P., Heinonen, M., Helander, I.M., Oksman-Caldentey, K.-M. and Puupponen-Pimiä, R.H. (2006) Berry phenolics: antimicrobial properties and mechanisms of action against severe human pathogens. Nutr Cancer 54, 18-32.
39. Pottier-Alapetite, G. (1981) Flora of Tunisia. Angiosperms Dicotyledons gamopetalous. Tunisia: Official Printing Works of Tunisia Republic, Tunis.
40. Satooka, H. and Kubo, I. (2012) Effects of thymol on B16-F10 melanoma cells. J Agric Food Chem 60, 2746-2752.
41. Schulz, H., Ozkan, G., Baranska, M., Kruger, H. and Ozcan, M. (2005) Characterisation of essential oil plants from Turkey by IR and Raman spectroscopy. Vib Spectrosc 39, 249-256.
42. Sertel, S., Eichhorn, T., Plinkert, P. and Efferth, T. (2011) Cytotoxicity of Thymus vulgaris essential oil towards human oral cavity squamous cell carcinoma. Anticancer Res 31, 81-88.
43. Sharon, N. and Ofek, I. (2002) Fighting infectious diseases with inhibitors of microbial adhesion to host tissues. Crit Rev Food Sci Nutr 42, 267-272.
44. Skoula, M. and Grayer, R.J. (2005) Volatile oils of Coridothymus capitatus, Satureja thymbra, Satureja spinosa and Thymbra calostachya (Lamiaceae) from Crete. Flavour Fragr J 20, 573-576.
45. Sokovic, M., Tzakou, O., Pitarakoli, D. and Couladis, M. (2002) Antifungal activities of selected aromatic plants growing wild in Greece. Nahrung 46, 317-320.
46. Todoroki, K., Mukai, T., Sato, S. and Toba, T. (2001) Inhibition of adhesion of food-born pathogens to Caco-2 cells by Lactobacillus. J Appl Microbiol 91, 154-159.
47. Tsukatani, T., Suenaga, H., Shiga, M., Noguchi, K., Ishiyama, M., Ezoe, T. and Matsumoto, K. (2012) Comparison of the WST-8 colorimetric method and the CLSI broth microdilution method for susceptibility testing against drug- resistant bacteria. J Microbiol Methods 90, 160-166.
48. Upadhyay, A., Johny, A.K., Amalaradjou, M.A., Ananda Baskaran, S., Kim, K.S. and Venkitanarayanan, K. (2012) Plant-derived antimicrobials reduce Listeria monocytogenes virulence factors in vitro, and down-regulate expression of virulence genes. Int J Food Microbiol 157, 88-94.
49. Upadhyay, A., Upadhyaya, I., Johny, A.K. and Venkitanarayanan, K. (2013) Antibiofilm effect of plant derived antimicrobials on Listeria monocytogenes. Food Microbiol 36, 79-89.
50. Vardar-Ünlü, G., Candan, F., Sökmen, A., Daferera, D., Polissiou, M., Sökmen, M., Dönmez, E. and Tepe, B. (2003) Antimicrobial and antioxidant activity of the essential oil and methanol extracts of Thymus pectinatus Fish. et Mey. var. pectinatus (Lamiaceae). J Agric Food Chem 51, 63-67.
51. Wang, W., Wu, N., Zu, Y.G. and Fu, Y.J. (2008) Antioxidative activity of Rosmarinus officinalis L. essential oil compared to its main components. Food Chem 108, 1019-1022.